| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,695,588 | +A | intergenic (‑45/‑213) | purL ← / → mltF | phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,695,585 | 1 | . | A | 97.6% | 138.3 / ‑3.9 | 42 | intergenic (‑42/‑216) | purL/mltF | phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base A (20/21); minor base G (0/1); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01 | |||||||||||
GTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGG‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATG > NC_000913/2695451‑2695721 | gtgAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGG‑‑aaaac > 2:429511/1‑138 (MQ=255)gtgAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGG‑‑aaaac < 2:728147/140‑3 (MQ=255) ccGGGAGCCTGGCAGCCTGAACACGTGCCATCAGTTTGTTGATTCGGAATGCCGACCGTGCAGGCGAACCACGCAGAATTTCCATCATAAGACTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCg < 1:62042/140‑1 (MQ=255) ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCg < 2:418643/140‑1 (MQ=255) ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCg < 1:734932/140‑1 (MQ=255) ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCg < 2:691607/140‑1 (MQ=255) ccGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCg < 2:831056/140‑1 (MQ=255) ggAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCa > 1:441998/1‑140 (MQ=255) ggAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCa > 1:597180/1‑140 (MQ=255) ggAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCa > 2:193396/1‑140 (MQ=255) gAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCAt < 1:800997/140‑1 (MQ=255) ccTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATc > 1:3822/1‑140 (MQ=255) aaCGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATgc < 2:97001/140‑1 (MQ=255) gcagTTTGGTGAATCGGAATGGCGACAGGTCAGGCGAACCACGGAGAATTTCCATCATTAGTCTCTCGTCCTCTAAACCTTCCGTGTTCCCAAG‑GGGGGGGAAAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGa < 2:200894/140‑1 (MQ=255) ttCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTg > 1:402594/1‑140 (MQ=255) cGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAGTGGGGGA‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGa > 2:576713/1‑140 (MQ=255) ggAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa < 2:597180/140‑1 (MQ=255) ggAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGaa < 2:648190/140‑1 (MQ=255) cGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAAt < 2:441998/140‑1 (MQ=255) cAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCAc < 1:429511/140‑1 (MQ=255) gTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCa > 2:251084/1‑140 (MQ=255) cAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCg < 2:909054/140‑1 (MQ=255) aGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGt > 1:106001/1‑140 (MQ=255) aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAAtt > 1:641825/1‑140 (MQ=255) aaCCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAAtt > 1:873823/1‑140 (MQ=255) aCGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCa < 1:12854/124‑1 (MQ=255) aCGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCa > 2:12854/1‑124 (MQ=255) cGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCaa > 1:612423/1‑140 (MQ=255) cAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAAcg > 1:867635/1‑140 (MQ=255) ttCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTgcc > 1:319847/1‑140 (MQ=255) atcatAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCa < 2:590483/140‑1 (MQ=255) tcatAAGTCGCTCGTCTTCTAAGCTTTCGGTGTACACAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCGCCATCGTGAATTAGCAACGCGTGCCGCCaa < 2:195039/140‑1 (MQ=255) atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATg > 2:98264/1‑140 (MQ=255) atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATg > 1:280890/1‑140 (MQ=255) atAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATg > 2:291606/1‑140 (MQ=255) tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg < 1:98264/140‑1 (MQ=255) tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg < 1:291606/140‑1 (MQ=255) tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg < 1:508233/140‑1 (MQ=255) tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg < 2:705373/140‑1 (MQ=255) tAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg < 2:730026/140‑1 (MQ=255) ctcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg < 2:911387/133‑1 (MQ=255) ctcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATgg > 1:911387/1‑133 (MQ=255) tcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaata > 1:367235/1‑140 (MQ=255) tcGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGtaata > 2:880123/1‑140 (MQ=255) tCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg > 2:69189/1‑140 (MQ=255) tCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg > 1:609605/1‑140 (MQ=255) tCGGTGTACCCAAG‑GGGGGGA‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGgcg < 2:9093/140‑1 (MQ=255) g‑ggggga‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCaaa > 1:814073/1‑140 (MQ=255) gggga‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAAt > 1:533871/1‑140 (MQ=255) gggga‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAAt > 2:212689/1‑140 (MQ=255) gggga‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAAt > 1:644125/1‑140 (MQ=255) ggga‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATg < 2:402594/140‑1 (MQ=255) | GTGAACCGGGAGCCTGGCAGCCTGAAAACGTGCCAGCAGTTTGTTGATTCGGAATGCCGACAGTGCAGGCGAACCACGCAGAATTTCCATCATAAGTCTCTCGTCTTCTAAGCTTTCGGTGTACCCAAG‑GGGGGG‑‑AAACGGGCGTCATTATAAAGAATCTGATGCGCTGACGAAACCGTTTGCGTGGAAATAAAATCACCATCGTGAATTAGCAACGCGTGCCGCCAATGGCTGTAATAAGTTGCCATCTGGCGCAGGTTTACGCAAAATG > NC_000913/2695451‑2695721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |