Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	348,325	+G	coding (119/1587 nt)	prpR ←	DNA‑binding transcriptional dual regulator PrpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	348,321	1	.	G	100.0%	192.3 / NA	52	P41P (CCT→CCC)	prpR	DNA‑binding transcriptional dual regulator PrpR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (27/25);  total (27/25)

GTTTAATCAAAATAACTGGCACTGACAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATA‑GGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAA  >  NC_000913/348182‑348455                                                                                                                                             |                                                                                                                                       gTTTAATCAAAATAACTGGCACTGACAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATa                                                                                                                                         <  1:349774/140‑1 (MQ=255)           aaTAACTGGCACTGACAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATg                                                                                                                               <  1:497501/140‑1 (MQ=255)            aTAACTGGCACTGACAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGt                                                                                                                              >  2:249545/1‑140 (MQ=255)                 tGGCACTGACAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGcc                                                                                                                         <  2:250609/140‑1 (MQ=255)                         aCAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATc                                                                                                                 <  1:529867/140‑1 (MQ=255)                         aCAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATc                                                                                                                 >  1:356074/1‑140 (MQ=255)                             gcggcTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAAc                                                                                                             >  2:475570/1‑140 (MQ=255)                             gcggcTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAAc                                                                                                             >  2:356755/1‑140 (MQ=255)                               ggcTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTc                                                                                                           <  2:679157/140‑1 (MQ=255)                                   tttCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAgg                                                                                                       >  1:624014/1‑140 (MQ=255)                                    ttCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGc                                                                                                      >  1:269512/1‑140 (MQ=255)                                              cgcCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATcgcg                                                                                            >  1:132830/1‑140 (MQ=255)                                              cgcCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATcgcg                                                                                            >  1:853006/1‑140 (MQ=255)                                              cgcCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATcgcg                                                                                            >  1:711710/1‑140 (MQ=255)                                              cgcCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATcgcg                                                                                            >  2:396578/1‑140 (MQ=255)                                              cgcCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATcgcg                                                                                            >  2:704940/1‑140 (MQ=255)                                              cgcCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATcgcg                                                                                            >  1:222426/1‑140 (MQ=255)                                                ccGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGaa                                                                                          <  2:376003/140‑1 (MQ=255)                                                 cGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGaaa                                                                                         >  2:360095/1‑140 (MQ=255)                                                             ccGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAg                                                                             <  1:396578/140‑1 (MQ=255)                                                             ccGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAg                                                                             <  2:222426/140‑1 (MQ=255)                                                             ccGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAg                                                                             >  1:242432/1‑140 (MQ=255)                                                             ccGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAg                                                                             >  1:96622/1‑140 (MQ=255)                                                             ccGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAg                                                                             <  1:704940/140‑1 (MQ=255)                                                              cgcgATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAgg                                                                            <  2:907113/140‑1 (MQ=255)                                                               gcgATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGgc                                                                           <  1:297558/140‑1 (MQ=255)                                                               gcgATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGgc                                                                           <  1:587444/140‑1 (MQ=255)                                                                cgATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGgcg                                                                          <  1:249545/140‑1 (MQ=255)                                                                   tgatgGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGt                                                                       >  2:873577/1‑140 (MQ=255)                                                                     atgGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTa                                                                     >  2:828953/1‑140 (MQ=255)                                                                           tCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATa                                                               <  2:853006/140‑1 (MQ=255)                                                                                      gttTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATa                                                    <  2:624014/140‑1 (MQ=255)                                                                                        ttGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAAc                                                  <  1:424140/140‑1 (MQ=255)                                                                                                ttCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTgt                                          <  2:242432/140‑1 (MQ=255)                                                                                                 tCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACGGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTgtc                                         >  1:491830/1‑140 (MQ=255)                                                                                                       ggATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCAtt                                   <  1:828953/140‑1 (MQ=255)                                                                                                       ggATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCAtt                                   <  1:356755/140‑1 (MQ=255)                                                                                                        gATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTa                                  >  2:345974/1‑140 (MQ=255)                                                                                                          tGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACGGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAg                                <  2:491830/140‑1 (MQ=255)                                                                                                           gTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGc                               <  1:475570/140‑1 (MQ=255)                                                                                                           gTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGc                               >  2:181402/1‑140 (MQ=255)                                                                                                           gTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGc                               >  2:647135/1‑140 (MQ=255)                                                                                                             aGGTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCg                             >  1:659453/1‑140 (MQ=255)                                                                                                               gTCACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCgtg                           <  1:269282/140‑1 (MQ=255)                                                                                                                 cACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCgtggt                         <  1:292755/140‑1 (MQ=255)                                                                                                                 cACTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCgtggt                         <  1:192021/140‑1 (MQ=255)                                                                                                                   cTGCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGtggtgg                       >  2:218682/1‑140 (MQ=255)                                                                                                                     gCTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGAt                     >  2:736421/1‑140 (MQ=255)                                                                                                                      cTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGAtg                    >  1:449281/1‑140 (MQ=255)                                                                                                                      cTTTTTCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATAACGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGAtg                    >  2:231671/1‑140 (MQ=255)                                                                                                                        ttttCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCGAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCATCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGAtgtg                  >  2:634989/1‑140 (MQ=255)                                                                                                                        ttttCAAAGCCAAGCTGAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGAtgtg                  <  2:356074/140‑1 (MQ=255)                                                                                                                                        gAATAGGGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCaa  <  2:906261/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       GTTTAATCAAAATAACTGGCACTGACAGGCGGCTTTTCAGGTACGCGCCGTTAGAGCCAGCCGCGATGATGGCGTCACAGCGTTCGTTTGCCAGTTTCTTGCGGATGTAGGTCACTGCTTTTTCAAAGCCAAGCTGAATA‑GGGGTAATGTTCGCCAGGTGATCAAACTCGAGGCTGATATCGCGAAACAGCTCGAACAGGCGCGTTACAGATACCGTCCAGATAACCGGTTTGTCGTCATTAAGCCGTGGTGGATGTGCCATAGCGCACCGCAA  >  NC_000913/348182‑348455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑