| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,947,713 | (T)7→8 | intergenic (+30/+44) | metV → / ← amiC | tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,947,706 | 1 | . | T | 92.9% | 84.4 / ‑0.0 | 28 | intergenic (+23/+51) | metV/amiC | tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (15/11); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTG‑‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTCCTGCTG > NC_000913/2947599‑2947837 | tACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAgc > 1:249564/1‑140 (MQ=255) aCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGTACACCCA‑AAAGGGGGA‑TTTTTTTGATTCTGGGCTCCCA‑TAAAAAAGAGCCCTTc > 2:599302/1‑140 (MQ=17) aCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTc > 2:517517/1‑140 (MQ=255) gcgGGGTGGAGCAGTCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAg < 2:905115/140‑1 (MQ=255) cgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAgc > 1:654956/1‑140 (MQ=255) cgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTG‑‑TTTTTTTGTTTTTTGTCTTCCATTAAAAAAGCCCCAtcaacc > 1:316395/1‑135 (MQ=18) ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAgcg < 1:599302/140‑1 (MQ=255) gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTG‑‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCttttt > 2:728251/1‑140 (MQ=255) gcagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTa < 1:791803/140‑1 (MQ=255) ccTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAgc > 2:817734/1‑127 (MQ=255) ccTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAgc < 1:817734/127‑1 (MQ=255) aGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCtt < 1:379569/140‑1 (MQ=255) cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGATCACCCT‑AACGGGGGA‑TTATTTTGATTCGGGTCGCCCC‑TAAAAAAGAGCCATTCAGCCCCTTATTATCATCCCCTtctgt > 2:341871/1‑138 (MQ=21) cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGTTTTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTtctc > 2:605053/1‑140 (MQ=255) cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCg > 2:204414/1‑140 (MQ=255) tcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTTTACACGCTGAAGGGGGA‑‑TTTTTTTGATGCTGGGCGCCCA‑TAAAAGAGCGCCATTCAGCCCCATTTTATCATCCCCTTCTCGcc > 2:379569/1‑140 (MQ=18) cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCa < 2:654956/140‑1 (MQ=255) aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCgg > 1:909146/1‑140 (MQ=255) aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCgg > 1:261597/1‑140 (MQ=255) aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCgg > 1:358888/1‑140 (MQ=255) ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCa < 2:249564/140‑1 (MQ=255) ggTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCa > 2:595032/1‑140 (MQ=255) gtcgGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAAt < 1:341871/140‑1 (MQ=255) tcgGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATa > 1:566909/1‑140 (MQ=255) tcgGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATa > 1:519227/1‑140 (MQ=255) gCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCg < 2:358888/140‑1 (MQ=255) gCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCg > 1:618053/1‑140 (MQ=255) ccAATTATTGAACACCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGa < 1:595032/140‑1 (MQ=255) cacCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTc > 2:575216/1‑140 (MQ=255) acCCT‑AACGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTcc < 2:566909/140‑1 (MQ=255) aaCGGGTGT‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCACCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTCctgctg > 1:121146/1‑140 (MQ=255) | TACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATTGAACACCCT‑AACGGGTG‑‑TTTTTTTGTTTCTGGTCTCCCA‑TAAAAAAGCGCCATTCAGCGCCTTTTTATCATCCCCTTCTCGCCAGCGTCGCCCCATCGGCAAAATACGCTTTAATCCCCGCAAGAATAGACTCCGCAACTTCCTGCTG > NC_000913/2947599‑2947837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |