| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,999,680 | (G)7→8 | coding (212/756 nt) | ygeR ← | LysM domain‑containing putative peptidase lipoprotein YgeR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,999,673 | 1 | . | G | 97.9% | 166.7 / ‑3.2 | 48 | coding (219/756 nt) | ygeR | LysM domain‑containing putative peptidase lipoprotein YgeR |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (17/30); total (17/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
GGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGC > NC_000913/2999536‑2999806 | ggcCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑gg > 1:113738/1‑140 (MQ=255) gatTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑GGGGGGGgaaa < 1:608415/140‑2 (MQ=255) ggTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGt < 1:782124/140‑1 (MQ=255) gCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTc > 1:687468/1‑140 (MQ=255) cGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACg < 2:700529/140‑1 (MQ=255) aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTGGCGCCAGTTCTTTTACGCt > 1:570276/1‑140 (MQ=255) aTGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCt < 2:142176/140‑1 (MQ=255) tGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTg > 2:370643/1‑140 (MQ=255) gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg > 2:610540/1‑140 (MQ=255) gCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTgg > 1:12962/1‑140 (MQ=255) ggTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGtt > 2:611959/1‑140 (MQ=255) ttGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCg > 2:763140/1‑140 (MQ=255) gTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgc < 1:293095/140‑1 (MQ=255) ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg < 2:612576/140‑1 (MQ=255) ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg < 1:710310/140‑1 (MQ=255) ttGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTgcg < 2:12962/140‑1 (MQ=255) gATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 2:47471/140‑1 (MQ=255) gATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGcgcg < 1:570193/140‑1 (MQ=255) tGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAAt < 1:610540/140‑1 (MQ=255) ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa < 1:259790/140‑1 (MQ=255) ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa < 2:325320/140‑1 (MQ=255) ggCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATa < 2:303525/140‑1 (MQ=255) ggTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGa < 2:113738/140‑1 (MQ=255) gTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGat < 1:138739/140‑1 (MQ=255) gtAATCCTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccc < 2:685878/140‑1 (MQ=255) gtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccc < 2:872912/140‑1 (MQ=255) aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccccc < 1:370643/140‑1 (MQ=255) aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccccc > 1:458561/1‑140 (MQ=255) tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑cccccccgtt > 2:495213/1‑136 (MQ=255) gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑cccccccgttt > 2:241527/1‑135 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc > 1:835716/1‑140 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc > 1:516760/1‑140 (MQ=255) cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc > 2:568612/1‑140 (MQ=255) tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa < 2:201429/140‑1 (MQ=255) tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa < 1:106644/140‑1 (MQ=255) ccacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACg < 1:763140/140‑1 (MQ=255) cacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACgg > 1:758795/1‑140 (MQ=255) acccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGa > 1:472447/1‑140 (MQ=255) cccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAg < 2:516760/140‑1 (MQ=255) ttttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGa < 1:256745/140‑1 (MQ=255) tttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa > 1:848075/1‑140 (MQ=255) tAGTTTCTGACCATCTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 2:195371/140‑1 (MQ=255) tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 2:472447/140‑1 (MQ=255) aGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa < 2:671382/139‑1 (MQ=255) aGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa > 1:671382/1‑139 (MQ=255) tCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc < 1:219991/140‑1 (MQ=255) tCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGgat < 2:687468/140‑1 (MQ=255) tgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAACTACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGc < 2:801561/140‑1 (MQ=255) tgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGc < 2:458561/140‑1 (MQ=255) | GGCCAGGATGATTTCGGTACCGCTGATGACGGTGTAACCGATGCGGTTTTGGTCGTTGATTTGGCGGTTGATTTACGTGTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGC > NC_000913/2999536‑2999806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |