Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,999,755	(C)6→7	coding (137/756 nt)	ygeR ←	LysM domain‑containing putative peptidase lipoprotein YgeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,999,749	1	.	C	100.0%	127.4 / NA	37	D48G (GAT→GGT)	ygeR	LysM domain‑containing putative peptidase lipoprotein YgeR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (18/19);  total (18/19)

GTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCC  >  NC_000913/2999614‑2999883                                                                                                                                          |                                                                                                                                       gtAATCCTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccc                                                                                                                                     <  2:685878/140‑1 (MQ=255) gtAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccc                                                                                                                                     <  2:872912/140‑1 (MQ=255)   aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccccc                                                                                                                                   <  1:370643/140‑1 (MQ=255)   aaTACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑ccccc                                                                                                                                   >  1:458561/1‑140 (MQ=255)        tGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑cccccccgtt                                                                                                                              >  2:495213/1‑136 (MQ=255)         gCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑cccccccgttt                                                                                                                             >  2:241527/1‑135 (MQ=255)          cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc                                                                                                                             >  1:516760/1‑140 (MQ=255)          cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc                                                                                                                             >  1:835716/1‑140 (MQ=255)          cTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTc                                                                                                                             >  2:568612/1‑140 (MQ=255)           tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa                                                                                                                            <  2:201429/140‑1 (MQ=255)           tACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCa                                                                                                                            <  1:106644/140‑1 (MQ=255)                      ccacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACg                                                                                                                 <  1:763140/140‑1 (MQ=255)                       cacccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACgg                                                                                                                >  1:758795/1‑140 (MQ=255)                        acccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGa                                                                                                               >  1:472447/1‑140 (MQ=255)                         cccaGTTTTAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAg                                                                                                              <  2:516760/140‑1 (MQ=255)                              ttttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGa                                                                                                         <  1:256745/140‑1 (MQ=255)                               tttAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGaa                                                                                                        >  1:848075/1‑140 (MQ=255)                                 tAGTTTCTGACCATCTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  2:195371/140‑1 (MQ=255)                                 tAGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  2:472447/140‑1 (MQ=255)                                  aGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      <  2:671382/139‑1 (MQ=255)                                  aGTTTCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATa                                                                                                      >  1:671382/1‑139 (MQ=255)                                      tCTGACCAACTTCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTc                                                                                                 <  1:219991/140‑1 (MQ=255)                                                 tCAATGGTGTAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGgat                                                                                      <  2:687468/140‑1 (MQ=255)                                                       tgtaagggggggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTg                                                                                >  1:565202/8‑140 (MQ=255)                                                        tgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAACTACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGc                                                                               <  2:801561/140‑1 (MQ=255)                                                        tgtAAGGGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGc                                                                               <  2:458561/140‑1 (MQ=255)                                                          taaggggggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTa                                                                             >  1:402366/5‑140 (MQ=255)                                                               ggggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGa                                                                        >  1:583709/1‑140 (MQ=255)                                                                gggggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGaa                                                                       <  2:583709/140‑1 (MQ=255)                                                                  gggAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACa                                                                     >  2:356708/1‑140 (MQ=255)                                                                             ttCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCaaaa                                                          >  1:779720/1‑140 (MQ=255)                                                                                   cgcgCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCTCGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTc                                                    <  2:583580/140‑1 (MQ=255)                                                                                   cgcgCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTc                                                    <  1:492933/140‑1 (MQ=255)                                                                                             cTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaa                                          <  2:835716/140‑1 (MQ=255)                                                                                                ttACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATaacaa                                       <  1:241527/140‑1 (MQ=255)                                                                                                         ttCCCGTGGTGCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGAt                              <  1:495213/140‑1 (MQ=255)                                                                                                              gtggtgCGCGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCa                         <  2:779720/140‑1 (MQ=255)                                                                                                                   gcgcGAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAt                    >  2:897510/1‑140 (MQ=255)                                                                                                                    cgcgAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAtt                   >  2:272786/1‑140 (MQ=255)                                                                                                                    cgcgAAATACGATATAGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGAtt                   <  1:568612/140‑1 (MQ=255)                                                                                                                                   aGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTc    >  1:759014/1‑140 (MQ=255)                                                                                                                                   aGCGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTc    >  1:227003/1‑140 (MQ=255)                                                                                                                                     cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  2:323695/1‑140 (MQ=255)                                                                                                                                     cGTATCCCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTccc  >  1:883735/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GTAATACTGCTACTTTTCGCCCCACCCAGTTTTAGTTTCTGACCAACTTCAATGGTGTAA‑GGGGGGGAAATGCCGTTCAGTCGCGCCAGTTCTTTTACGCTGGTTCCCGTGGTGCGCGAAATACGATATAGCGTAT‑CCCCCCGTTTCACGGTGTAAACGGAGCCGGAATACGTTCCTGTATCGGATGATTTGCTACCCGAACAGCCCGCCAAAAGCAGTCCAACCGATAACAACATCACGATACCCAGAGATTTTTTATTCAGGCGTCCC  >  NC_000913/2999614‑2999883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑