Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,102,340	(C)6→7	coding (513/720 nt)	trmI ←	tRNA m(7)G46 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,102,334	1	.	C	100.0%	171.7 / NA	48	G173G (GGC→GGG)	trmI	tRNA m(7)G46 methyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (21/27);  total (21/27)

TTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAA  >  NC_000913/3102199‑3102464                                                                                                                                         |                                                                                                                                   tttCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑cccc                                                                                                                                <  2:71347/140‑1 (MQ=255) tttCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑cccc                                                                                                                                <  2:561114/140‑1 (MQ=255)                        gACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTAAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTAc                                                                                                          <  2:221056/140‑1 (MQ=255)                                       cATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           <  2:221187/140‑1 (MQ=255)                                       cATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           <  2:419088/140‑1 (MQ=255)                                         ttcctctctGACAGGTTTTAATACCCGTCAATAGAAGAAATCACTCCAGGCATAGGTTCCGCTTAAGGTCCCCGGTCGGTGGCCTTATGAAATCGG‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAc                                                                                        <  2:434026/137‑1 (MQ=255)                                         ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCa                                                                                         >  2:95283/1‑140 (MQ=255)                                         ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCa                                                                                         >  2:613785/1‑140 (MQ=255)                                         ttGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCa                                                                                         >  1:42502/1‑140 (MQ=255)                                          tGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAc                                                                                        <  2:569019/140‑1 (MQ=255)                                           gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  1:410569/1‑140 (MQ=255)                                           gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  1:637642/1‑140 (MQ=255)                                           gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  1:264035/1‑140 (MQ=255)                                           gCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc                                                                                       >  2:771411/1‑140 (MQ=255)                                               tctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGaa                                                                                   <  2:632452/140‑1 (MQ=255)                                                ctGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAAc                                                                                  >  1:451396/1‑140 (MQ=255)                                                 tGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           <  2:188774/130‑1 (MQ=255)                                                 tGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg                                                                                           >  1:188774/1‑130 (MQ=255)                                                      ggTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACg                                                                            <  1:515161/140‑1 (MQ=255)                                                          tttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGt                                                                        <  2:431823/140‑1 (MQ=255)                                                             ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTtta                                                                     <  1:909848/140‑1 (MQ=255)                                                                 ccGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTtattat                                                                 >  1:368771/1‑140 (MQ=255)                                                                  cGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATg                                                                >  1:242308/1‑140 (MQ=255)                                                                         agaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTCTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCt                                                         <  2:637642/140‑1 (MQ=255)                                                                           aagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCttt                                                       >  2:50685/1‑140 (MQ=255)                                                                                 tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGTAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:50685/140‑1 (MQ=255)                                                                                 tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 >  1:211807/1‑140 (MQ=255)                                                                                 tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:226135/140‑1 (MQ=255)                                                                                 tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  2:439301/140‑1 (MQ=255)                                                                                 tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:440988/140‑1 (MQ=255)                                                                                 tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:92082/140‑1 (MQ=255)                                                                                 tCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCa                                                 <  1:642074/140‑1 (MQ=255)                                                                                  cACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCAc                                                >  2:783747/1‑140 (MQ=255)                                                                                     ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACggg                                             >  2:367663/1‑140 (MQ=255)                                                                                         aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAg                                         <  1:619216/140‑1 (MQ=255)                                                                                          gCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAgg                                        >  1:320330/1‑140 (MQ=255)                                                                                              atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  1:783747/140‑1 (MQ=255)                                                                                              atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  1:453298/140‑1 (MQ=255)                                                                                              atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  2:211807/140‑1 (MQ=255)                                                                                              atGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaa                                    <  1:95283/140‑1 (MQ=255)                                                                                               tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGaaaa                                   >  2:216533/1‑140 (MQ=255)                                                                                                    cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGc                              >  1:714323/1‑140 (MQ=255)                                                                                                    cGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGc                              >  2:112122/1‑140 (MQ=255)                                                                                                       aTAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGc                           <  1:216533/140‑1 (MQ=255)                                                                                                          aGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCAcc                        <  2:264035/140‑1 (MQ=255)                                                                                                               cccAGTCGGTCGCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATgcg                   <  1:273019/140‑1 (MQ=255)                                                                                                                      ggtcgCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGaa            >  1:796770/1‑140 (MQ=255)                                                                                                                       gtcgCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAAt               >  2:379098/1‑136 (MQ=255)                                                                                                                       gtcgCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAAt               <  1:379098/136‑1 (MQ=255)                                                                                                                          gCCATATGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGt        <  1:771411/140‑1 (MQ=255)                                                                                                                                tGGAATACGCCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGaa  <  1:613785/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TTTCGTCACCGGACGTGATGCCGGACGCGGTACGTAATCATTGCTCTCTGACAGGTTTTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACG‑CCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTTTATTATGGCGCGCTTTGTGCCACGGGTCAGGGAAAAAGAGCTGCACCATGCGCAATGAATTGTCAGGAA  >  NC_000913/3102199‑3102464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑