Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT38.9% 13.3 / 11.0 18intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/11);  new base T (7/0);  total (7/11)
Fisher's exact test for biased strand distribution p-value = 3.14e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAA  >  NC_000913/3206298‑3206570
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aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGGTTTTTTCa                                                                                                                                       >  2:282166/1‑140 (MQ=255)
  aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTGCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAGa                                                                                                                                     >  2:176735/1‑140 (MQ=255)
    cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                                   <  1:899631/140‑1 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACaaaa                                                                                                                                >  1:272760/1‑139 (MQ=255)
              cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAaaaaaaaaaa                                                                                                                         >  2:556589/1‑140 (MQ=255)
              cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAaaaaaa                                                                                                                         >  1:922225/1‑140 (MQ=255)
                 tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAaaaaaaaaaaaaa                                                                                                                      >  2:400865/1‑137 (MQ=255)
                  gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                     <  1:134400/140‑1 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATACAAAAAAAAAAAAAGGAGTTgg                                                                                                               >  1:205364/1‑139 (MQ=255)
                                    ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                   <  1:392844/140‑1 (MQ=255)
                                      cTTATTTTCGCGTTGATTCACGAACTGTTAATTGCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                 <  1:176735/140‑1 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGAGGGAAAga                                                                                               >  2:47682/1‑139 (MQ=255)
                                                     aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                  <  1:222988/140‑1 (MQ=255)
                                                          cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                             <  2:922225/140‑1 (MQ=255)
                                                             aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                          <  1:400865/140‑1 (MQ=255)
                                                              cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                         <  2:880345/140‑1 (MQ=255)
                                                                               aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATACAAAAAAAGGAGATGGCACCTTAAGAGGAAGTAAAAAGCAAAAGGCAGGGAATAAAATGAGAAAGATTGTc                                                        >  2:178361/1‑140 (MQ=255)
                                                                                  gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAAATTGTGGTGCCCTGGAGCGCGAAAGGAATAAACAACAAGCAGTGAATAAGTTGACAGAGATTGGCGCt                                                     >  2:770597/1‑140 (MQ=255)
                                                                                    ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAAAAAAAATGGAGTTGGCATGATGTGGGAAAGAAATAAACAAAAAGCAGGGAATAAATTGACAGAGAATGTTGCTaa                                                   >  2:332260/1‑140 (MQ=255)
                                                                                               aaaacCCACATTTTTAGTGGTAGTTGGTGGCGGTGATTTTTTCCAGACAAATAAAAAATTTGGAGTGTCATTGATGAGCAAAAGTAATAAGAAACAGGAGGGAAATAGGTTGACGGAGATTGTCGTTATCTTTACCGCCa                                        <  2:421262/139‑1 (MQ=255)
                                                                                                               gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                        <  1:47682/140‑1 (MQ=255)
                                                                                                               gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                        <  1:332260/140‑1 (MQ=255)
                                                                                                                    aGTTTGTGGCGGTGAATTTTTTCACACAAAAACAAAAAATGGAGGTGGCCTGATGAGCGAAAAAAAAAAACAAAAAGCAGGGAATAAATTTACAAAGATTTTCGCTAAATTTACCCCCAAGATTTCTACCCCAATGCCtg                   >  2:262713/1‑140 (MQ=255)
                                                                                                                                     ttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACAGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa  <  1:556589/140‑1 (MQ=255)
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AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAA  >  NC_000913/3206298‑3206570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑