Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	59,287	T→C	intergenic (+8/+400)	yabQ → / ← rluA	protein YabQ/23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	59,287	0	T	C	100.0%	182.2 / NA	49	intergenic (+8/+400)	yabQ/rluA	protein YabQ/23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/27);  total (22/27)

GTAATTATTATTAATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTGCAGGACACGGATCACCGAGATGG  >  NC_000913/59151‑59420                                                                                                                                          |                                                                                                                                      gTAATTATTATTAATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGa                                                                                                                                    <  2:172771/140‑1 (MQ=255) gTAATTATTATTAATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGa                                                                                                                                    <  2:157862/140‑1 (MQ=255)  tAATTATTATTAATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGaa                                                                                                                                   <  2:329539/140‑1 (MQ=255)   aaTTATTATTAATAAAAT‑CCCCCCCAAGAAAATTGCTACTAAAGGTGTTGATACAGAAGAAGTTGCTACTAAAAAAGTACTGTTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTCAAg                                                                                                                                  >  2:366932/1‑140 (MQ=255)     ttattattaATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAggg                                                                                                                                >  1:401333/1‑140 (MQ=255)          attaATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTgg                                                                                                                           >  1:602347/1‑140 (MQ=255)           ttaATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGa                                                                                                                          >  2:225940/1‑140 (MQ=255)              ataaAATCCCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAAc                                                                                                                        >  1:902799/1‑140 (MQ=255)                          cccAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTc                                                                                                                <  1:17642/136‑1 (MQ=255)                          cccAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTc                                                                                                                >  2:17642/1‑136 (MQ=255)                             aaaaaaaTTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTa                                                                                                         >  2:608039/1‑140 (MQ=255)                                aaaaTTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCa                                                                                                      <  1:630534/140‑1 (MQ=255)                                          cTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTggg                                                                                            <  1:288404/140‑1 (MQ=255)                                           tAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGga                                                                                           <  2:386756/140‑1 (MQ=255)                                                  gTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTc                                                                                    >  2:66554/1‑140 (MQ=255)                                                   ttGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGc                                                                                                 <  2:568460/126‑1 (MQ=255)                                                   ttGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGc                                                                                                 >  1:568460/1‑126 (MQ=255)                                                   ttGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTca                                                                                   >  1:325725/1‑140 (MQ=255)                                                     gCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTcaca                                                                                 >  1:1123/1‑140 (MQ=255)                                                     gCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTcaca                                                                                 >  2:312198/1‑140 (MQ=255)                                                     gCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTcaca                                                                                 <  1:312198/140‑1 (MQ=255)                                                     gCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTcaca                                                                                 >  1:379080/1‑140 (MQ=255)                                                      cTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACaa                                                                                <  2:118937/140‑1 (MQ=255)                                                      cTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACaa                                                                                <  1:672265/140‑1 (MQ=255)                                                           gaagaaGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGa                                                                           <  2:401333/140‑1 (MQ=255)                                                                agTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGAc                                                                      >  2:22874/1‑140 (MQ=255)                                                                   tGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTg                                                                   >  2:349728/1‑140 (MQ=255)                                                                    gCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGc                                                                  >  1:196393/1‑140 (MQ=255)                                                                     ctactaAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCa                                                                 <  2:343478/140‑1 (MQ=255)                                                                       actaAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCatg                                                               <  2:368093/140‑1 (MQ=255)                                                                          aaaaaaGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCatgatg                                                            <  2:539669/140‑1 (MQ=255)                                                                          aaaaaaGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCatgatg                                                            <  2:902799/140‑1 (MQ=255)                                                                          aaaaaaGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCatgatg                                                            >  1:550030/1‑140 (MQ=255)                                                                            aaaaGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAg                                                          <  2:196393/140‑1 (MQ=255)                                                                                    tgctgAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGagaa                                                  <  2:294943/140‑1 (MQ=255)                                                                                          aCAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCt                                            >  2:339743/1‑140 (MQ=255)                                                                                                   tGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGc                                             >  1:126031/1‑130 (MQ=255)                                                                                                   tGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGc                                             <  2:126031/130‑1 (MQ=255)                                                                                                         cGCAATTATTGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTAAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGcgt                             >  1:544982/1‑140 (MQ=255)                                                                                                             attattGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTg                         <  1:660517/140‑1 (MQ=255)                                                                                                             attattGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTg                         <  2:737411/140‑1 (MQ=255)                                                                                                             attattGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTg                         <  1:22874/140‑1 (MQ=255)                                                                                                             attattGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTg                         <  1:385926/140‑1 (MQ=255)                                                                                                             attattGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTg                         <  1:608039/140‑1 (MQ=255)                                                                                                             attattGAATGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTg                         <  2:184201/140‑1 (MQ=255)                                                                                                                      tGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTGCAGGACACg                >  1:709127/1‑140 (MQ=255)                                                                                                                      tGAGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTGCAGGACACg                <  2:1123/140‑1 (MQ=255)                                                                                                                        aGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTGCAGGACACg                <  1:195946/138‑1 (MQ=255)                                                                                                                        aGCCAGAATAAGCTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTGCAGGACACg                >  2:195946/1‑138 (MQ=255)                                                                                                                                    cTAAGGCTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTGCAGGACACGGATCACCGAGATgg  >  2:277196/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GTAATTATTATTAATAAAAT‑CCCCCCCAAAAAAATTGATACTAAAGGAGTTGCTACTGAAGAAGTTGCTACTAAAAAAGTACTGCTGAACAAATTACTGACAACGCAATTATTGAATGAGCCAGAATAAGCTAAGGTTGAAGGGGCTGGAACGCCCCTTCAACCTTAGCAGTAGCGTGGGATGATTTCACAATTAGAAAGACCTGCATGATGAGCTAGAGAAGAGGCTAGTGACGCAAGGCGTCGTGCAGGACACGGATCACCGAGATGG  >  NC_000913/59151‑59420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑