Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,161	A→G	V58A (GTC→GCC)	yaiY ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,161	0	A	G	100.0%	208.9 / NA	57	V58A (GTC→GCC)	yaiY	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

ACTGGCGGCGATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATT  >  NC_000913/399027‑399293                                                                                                                                       |                                                                                                                                       aCTGGCGGCGATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGc                                                                                                                                   >  2:576383/1‑140 (MQ=255) aCTGGCGGCGATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGc                                                                                                                                   >  1:328986/1‑140 (MQ=255) aCTGGCGGCGATGTCCCCAGTGCAGCCATACCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGc                                                                                                                                   >  2:886113/1‑140 (MQ=255)       ggcgATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTaaa                                                                                                                             <  2:374170/140‑1 (MQ=255)        gcgATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAAc                                                                                                                            >  2:730461/1‑140 (MQ=255)        gcgATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAAc                                                                                                                            >  2:162625/1‑140 (MQ=255)        gcgATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAAc                                                                                                                            >  2:209071/1‑140 (MQ=255)         cgATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACg                                                                                                                           <  2:132935/140‑1 (MQ=255)         cgATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACg                                                                                                                           <  2:33410/140‑1 (MQ=255)               cGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATa                                                                                                                     <  1:196835/140‑1 (MQ=255)                    gTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGc                                                                                                                <  1:302146/140‑1 (MQ=255)                          ccATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGgcg                                                                                                          <  2:130705/140‑1 (MQ=255)                            aTAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCa                                                                                                        <  2:794153/140‑1 (MQ=255)                             tAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCAt                                                                                                       <  2:736228/140‑1 (MQ=255)                             tAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCAt                                                                                                       <  1:540910/140‑1 (MQ=255)                              aGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATg                                                                                                      <  1:674573/140‑1 (MQ=255)                              aGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATg                                                                                                      >  2:96484/1‑140 (MQ=255)                              aGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATg                                                                                                      <  1:508885/140‑1 (MQ=255)                                cgcCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑Ca                                                                                                  <  2:114655/140‑1 (MQ=255)                                      cacTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACa                                                                                            <  2:636258/140‑1 (MQ=255)                                           cAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTc                                                                                       >  2:723218/1‑140 (MQ=255)                                               aaTGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCa                                                                                   >  2:890433/1‑140 (MQ=255)                                                   aGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTg                                                                               <  1:436931/140‑1 (MQ=255)                                                              taAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGccc                                                                    <  1:96484/140‑1 (MQ=255)                                                              taAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGccc                                                                    <  1:880517/140‑1 (MQ=255)                                                                   aCGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAg                                                               >  2:511563/1‑140 (MQ=255)                                                                   aCGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAg                                                               >  1:141023/1‑140 (MQ=255)                                                                       gATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAATGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCaaa                                                           <  1:576383/140‑1 (MQ=255)                                                                       gATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCaaa                                                           >  1:266927/1‑140 (MQ=255)                                                                       gATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCaaa                                                           >  1:521449/1‑140 (MQ=255)                                                                       gATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCaaa                                                           >  2:623906/1‑140 (MQ=255)                                                                       gATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCaaa                                                           >  2:725538/1‑140 (MQ=255)                                                                       gATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCaaa                                                           >  1:644890/1‑140 (MQ=255)                                                                            gCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGc                                                      <  1:590078/140‑1 (MQ=255)                                                                             cccGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGca                                                     <  1:621804/140‑1 (MQ=255)                                                                             cccGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGca                                                     >  2:265246/1‑140 (MQ=255)                                                                                 aaaaTGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCacaaa                                                 >  1:359432/1‑140 (MQ=255)                                                                                  aaaTGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCacaaac                                                <  1:890433/140‑1 (MQ=255)                                                                                       gTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGc                                           >  1:825276/1‑140 (MQ=255)                                                                                           cGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAg                                       <  1:209071/140‑1 (MQ=255)                                                                                           cGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAg                                       <  1:730461/140‑1 (MQ=255)                                                                                            gACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAgg                                      <  2:328986/140‑1 (MQ=255)                                                                                            gACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAgg                                      <  1:162625/140‑1 (MQ=255)                                                                                                   aaaCCAATTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGtt                               >  1:287208/1‑140 (MQ=255)                                                                                                         aTTTCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAgg                         <  1:511563/140‑1 (MQ=255)                                                                                                           ttCCACCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCg                       <  1:886113/140‑1 (MQ=255)                                                                                                               aCCCGTGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGa                   <  2:287208/140‑1 (MQ=255)                                                                                                                    tGGGCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAgg              >  1:663/1‑140 (MQ=255)                                                                                                                      ggcaaCCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGc                        >  2:762216/5‑128 (MQ=255)                                                                                                                      ggCGACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCa            >  2:608770/1‑140 (MQ=255)                                                                                                                      ggCAACCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGc                        <  1:762216/128‑1 (MQ=255)                                                                                                                          aCCTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGc        >  2:434092/1‑140 (MQ=255)                                                                                                                           ccTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATGCACACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTg         >  2:655961/1‑140 (MQ=255)                                                                                                                           ccTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCg       <  2:644890/140‑1 (MQ=255)                                                                                                                           ccTGTTTCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCg       >  1:858356/1‑140 (MQ=255)                                                                                                                                ttCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATAtt  >  1:583717/1‑140 (MQ=255)                                                                                                                                ttCCTGGGCCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATAtt  <  1:265246/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ACTGGCGGCGATGTCGCCAGTGCAGCCATAGCGCCACCACTGCAAAAATGAGGCAGCCTACTAAAAACGGGATCAGCCCGAAAATGGTGCCGACACCTAAACCAATTTCCACCCGTGGGCGACCTGTTTCCTGGACCTGCATTAAACGCTCATAGACGCCGGGCGCATG‑‑CACTAACAGGTTCAGCAATTGCGCCCCAGCCCAAAAGCAAAACAGCACAAACAACGCATAGGCAATGTTGCCAGGCGTTGAAGAGGCATTGCGATATT  >  NC_000913/399027‑399293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑