Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,852	G→T	intergenic (+241/‑190)	yhdV → / → yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,852	0	G	T	100.0%	160.8 / NA	46	intergenic (+241/‑190)	yhdV/yhdW	lipoprotein YhdV/putative ABC transporter periplasmic binding protein YhdW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/25);  total (21/25)

TTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGA  >  NC_000913/3418717‑3418981                                                                                                                                        |                                                                                                                                  ttACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCa                                                                                                                               >  2:423090/1‑140 (MQ=255)      tCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGttt                                                                                                                          <  2:370328/140‑1 (MQ=255)       cgcgTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTc                                                                                                                         <  1:895803/140‑1 (MQ=255)       cgcgTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTc                                                                                                                         >  2:543670/1‑140 (MQ=255)         cgTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCAt                                                                                                                       >  2:748094/1‑140 (MQ=255)                      ctTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTg                                                                                                          >  1:354908/1‑140 (MQ=255)                         tCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCgg                                                                                                       >  1:356690/1‑140 (MQ=255)                             aaTAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGgcg                                                                                                   <  2:354908/140‑1 (MQ=255)                             aaTAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGgcg                                                                                                   <  2:356690/140‑1 (MQ=255)                              aTAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGgcgc                                                                                                  <  1:748094/140‑1 (MQ=255)                                aaCGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTg                                                                                                >  2:58828/1‑140 (MQ=255)                                  cGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCAtt                                                                                                           >  2:38502/1‑127 (MQ=255)                                  cGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCAtt                                                                                                           <  1:38502/127‑1 (MQ=255)                                          tttaatttTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTtattat                                                                                      >  1:563929/1‑140 (MQ=255)                                            taatttTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTAttt                                                                                    <  1:521411/140‑1 (MQ=255)                                            taatttTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTAttt                                                                                    <  2:10565/140‑1 (MQ=255)                                              atttTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTAttt                                                                                    <  2:547398/138‑1 (MQ=255)                                              atttTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTAttt                                                                                    >  1:547398/1‑138 (MQ=255)                                                 ttCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCTGGGCGCTGTTTTtattat                                                                                      >  2:34509/1‑133 (MQ=255)                                                 ttCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCTGGGCGCTGTTTTtattat                                                                                      <  1:34509/133‑1 (MQ=255)                                                       cTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTacac                                                                         <  1:543670/140‑1 (MQ=255)                                                        ttCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACt                                                                        <  2:585766/140‑1 (MQ=255)                                                                  aaaTATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTg                                                              >  1:613510/1‑140 (MQ=255)                                                                                tAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGt                                                >  2:398153/1‑140 (MQ=255)                                                                                      tCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCaa                                          >  2:679935/1‑140 (MQ=255)                                                                                        attattCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCaaaa                                        <  1:679935/140‑1 (MQ=255)                                                                                          tattCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCACACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGc                                      >  2:383609/1‑140 (MQ=255)                                                                                           attCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCt                                     <  1:58828/140‑1 (MQ=255)                                                                                           attCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTCTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCACAAAAGCt                                     <  1:423090/140‑1 (MQ=255)                                                                                            ttCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTg                                    >  1:282569/1‑140 (MQ=255)                                                                                              cTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCAGTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGc                                  >  2:812242/1‑140 (MQ=255)                                                                                               tGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCa                                 <  1:593686/140‑1 (MQ=255)                                                                                                 cTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCact                               >  1:40953/1‑140 (MQ=255)                                                                                                     gTAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACtt                           <  2:563929/140‑1 (MQ=255)                                                                                                      tAAAACACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACctt                          >  2:341191/1‑140 (MQ=255)                                                                                                        aaaCACGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTg                        <  2:613510/140‑1 (MQ=255)                                                                                                          acacGCGTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCt                      <  2:282569/140‑1 (MQ=255)                                                                                                              gcgTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTtata                  <  1:355372/140‑1 (MQ=255)                                                                                                                gTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGTGACGCGAAATCGTGCGCAAAAGCTGGCACTACtttt                         >  1:321503/1‑131 (MQ=255)                                                                                                                gTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGTGACGCGAAATCGTGCGCAAAAGCTGGCACTACtttt                         <  2:321503/131‑1 (MQ=255)                                                                                                                gTAAATATTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAag                >  2:710715/1‑140 (MQ=255)                                                                                                                    atatTTTGCGCCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGa            <  1:783912/140‑1 (MQ=255)                                                                                                                            cgcCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTACGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCcaca    >  2:807967/1‑140 (MQ=255)                                                                                                                            cgcCAAAATGTGTCGCATGTTTCATTTTCACACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCcaca    <  1:383609/140‑1 (MQ=255)                                                                                                                             gcCAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCacag   <  2:264228/140‑1 (MQ=255)                                                                                                                              ccAAAATGTGTCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCacaga  <  1:912217/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  TTACATCGCGTTATTTCGCTGCTTTCGAAATAACGCATCAATTTAATTTTCACACTTCCCACCAGAAATATACTCACGCTAACAATCATTATTCTGCTTAGTAAAACACGCGTAAATATTTTGCGCCAAAATGTGGCGCATGTTTCATTTTCGCACCATTGCGGGGCGCTGTTTTTATTATTTCCTGTCTACACTCAGCTTATTGCGACGCGAAATCGTGCGCAAAAGCTGGCACTACTTTTGCTTATAAGAGGATGGCCACAGA  >  NC_000913/3418717‑3418981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑