Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,496,567	A→C	D6A (GAC→GCC)	nirD →	nitrite reductase subunit NirD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,496,567	0	A	C	100.0%	254.8 / NA	68	D6A (GAC→GCC)	nirD	nitrite reductase subunit NirD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/36);  total (32/36)

ACTTCATCAACAGCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGACATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGACCCGTTCTTCGA  >  NC_000913/3496436‑3496699                                                                                                                                    |                                                                                                                                     acTTCATCAACAGCGACAAGCGTGACCCGAACGTGCAGATGGCGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCa                                                                                                                              <  1:517696/140‑1 (MQ=255)  cTTCATCAACAGCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCaa                                                                                                                             <  2:884166/140‑1 (MQ=255)   ttCATCAACAGCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCaaa                                                                                                                            <  1:193582/140‑1 (MQ=255)    tcatcaACAGCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCaaaa                                                                                                                           >  1:378707/1‑140 (MQ=255)     catcaACAGCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAAt                                                                                                                          <  1:47005/140‑1 (MQ=255)           cAGCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGa                                                                                                                    >  1:273027/1‑140 (MQ=255)             gCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCa                                                                                                                              <  2:522616/128‑1 (MQ=255)             gCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCa                                                                                                                              >  1:522616/1‑128 (MQ=255)             gCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACa                                                                                                                  >  1:243720/1‑140 (MQ=255)             gCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACa                                                                                                                  >  2:480773/1‑140 (MQ=255)             gCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACa                                                                                                                  >  1:363980/1‑140 (MQ=255)             gCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACa                                                                                                                  >  2:455258/1‑140 (MQ=255)               gACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATc                                                                                                                <  2:383229/140‑1 (MQ=255)               gACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGACCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATc                                                                                                                <  1:316286/140‑1 (MQ=255)                aCAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATcc                                                                                                               <  1:58885/140‑1 (MQ=255)                 cAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATcct                                                                                                              <  2:378707/140‑1 (MQ=255)                   aGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATcctgc                                                                                                            >  2:394890/1‑140 (MQ=255)                         aCCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGaaa                                                                                                      >  2:378381/1‑140 (MQ=255)                                cGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGt                                                                                               <  1:686610/140‑1 (MQ=255)                                  tGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCt                                                                                             <  2:375567/140‑1 (MQ=255)                                      gATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcgc                                                                                         <  1:378381/140‑1 (MQ=255)                                       aTGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcgcg                                                                                        <  2:371494/140‑1 (MQ=255)                                       aTGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcgcg                                                                                        <  1:480773/140‑1 (MQ=255)                                       aTGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcgcg                                                                                        <  1:911355/140‑1 (MQ=255)                                       aTGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcgcg                                                                                        <  1:89189/140‑1 (MQ=255)                                       aTGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcgcg                                                                                        <  1:371646/140‑1 (MQ=255)                                       aTGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcgcg                                                                                        <  1:69562/140‑1 (MQ=255)                                            gCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGtt                                                                                   <  2:921730/140‑1 (MQ=255)                                            gCCAGAGCGCGAACAGCACCGTCCGACAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcg                                                                                          <  2:454506/133‑1 (MQ=255)                                            gCCAGAGCGCGAACAGCACCGTCCGACAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTgcg                                                                                          >  1:454506/1‑133 (MQ=255)                                              cAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAg                                                                                 <  1:394890/140‑1 (MQ=255)                                                gagCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGt                                                                               <  1:278800/140‑1 (MQ=255)                                                 agCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTg                                                                              >  1:274875/1‑140 (MQ=255)                                                     cgAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGa                                                                          >  2:512903/1‑140 (MQ=255)                                                     cgAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGa                                                                          >  1:389831/1‑140 (MQ=255)                                                     cgAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGa                                                                          >  1:804923/1‑140 (MQ=255)                                                                    ggCCACGCCGTATGAAAGTATCCCAGTAACTCTGGTGGAGGACAACGGATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGAGACGAGCAGGTCGCGAtttt                                                           <  2:639450/140‑1 (MQ=255)                                                                        aCGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGc                                                       <  1:110865/140‑1 (MQ=255)                                                                        aCGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGc                                                       <  2:243720/140‑1 (MQ=255)                                                                              tATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTcc                                                         <  2:719358/132‑1 (MQ=255)                                                                              tATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTcc                                                         >  1:719358/1‑132 (MQ=255)                                                                                tGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGTCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATca                                               <  1:835352/140‑1 (MQ=255)                                                                                  aaCGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATcaca                                             <  2:439793/140‑1 (MQ=255)                                                                                   aCGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAg                                            >  1:413045/1‑140 (MQ=255)                                                                                        tCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATc                                       >  2:896725/1‑140 (MQ=255)                                                                                           cAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAgg                                    <  1:871669/140‑1 (MQ=255)                                                                                           cAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAgg                                    <  1:455258/140‑1 (MQ=255)                                                                                           cAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAgg                                    <  1:452511/140‑1 (MQ=255)                                                                                             gTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGgtg                                  >  1:508995/1‑140 (MQ=255)                                                                                               aaCTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGtt                                >  2:871748/1‑140 (MQ=255)                                                                                                   ctGGTGGAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACAGCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCTGTATCTCAGCGGTCACGTGTTTGCg                            <  2:195149/140‑1 (MQ=255)                                                                                                    tggtggAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGa                           >  2:904764/1‑140 (MQ=255)                                                                                                     ggtggAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGAt                          >  1:623842/1‑140 (MQ=255)                                                                                                     ggtggAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGAt                          <  2:413045/140‑1 (MQ=255)                                                                                                       tggAGGACAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCa                        >  2:586818/1‑140 (MQ=255)                                                                                                        ggaggaCAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAg                       <  1:320923/140‑1 (MQ=255)                                                                                                          aggaCAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCa                     >  1:880457/1‑140 (MQ=255)                                                                                                            gaCAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAAc                   >  2:429585/1‑140 (MQ=255)                                                                                                            gaCAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAAc                   >  1:79451/1‑140 (MQ=255)                                                                                                            gaCAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAAc                   >  1:49806/1‑140 (MQ=255)                                                                                                             aCAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACa                  >  2:724322/1‑140 (MQ=255)                                                                                                              cAACGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACAt                 >  1:793748/1‑140 (MQ=255)                                                                                                                aCGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCg               >  1:723092/1‑140 (MQ=255)                                                                                                                 cGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGa              >  1:783034/1‑140 (MQ=255)                                                                                                                 cGCATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGa              >  1:597642/1‑140 (MQ=255)                                                                                                                   cATGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGAcc            <  1:724322/140‑1 (MQ=255)                                                                                                                     tGAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGACCCg          <  2:793748/140‑1 (MQ=255)                                                                                                                      gAGCCAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGACCCGt         <  1:429585/140‑1 (MQ=255)                                                                                                                          cAGTGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGACCCGttctt     <  2:880457/140‑1 (MQ=255)                                                                                                                             tGGAAAGCCATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGACCCGTTCTTCGa  >  2:541422/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     ACTTCATCAACAGCGACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCAGTAACTCTGGTGGAGGACAACGCATGAGCCAGTGGAAAGACATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCGCTGTTAGGTGACGAGCAGGTCGCGATTTTCCGCCCGTATCACAGCGATCAGGTGTTTGCGATCAGCAACATCGACCCGTTCTTCGA  >  NC_000913/3496436‑3496699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑