Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,522,499	A→G	S85P (TCA→CCA)	hofM ←	DNA utilization protein HofM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,522,499	0	A	G	100.0%	193.7 / NA	53	S85P (TCA→CCA)	hofM	DNA utilization protein HofM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/26);  total (27/26)

GGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGACCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCTTTGATAATGTCG  >  NC_000913/3522365‑3522629                                                                                                                                       |                                                                                                                                   ggCGGGACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGGCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGGAAAGGCCGcc                                                                                                                               >  2:414752/1‑139 (MQ=255)           agtgagtCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTTCTCCCGCCCACCAAGGGACATCGACGGGCGCGGAAAAGGCCGCTGTAATGTGCg                                                                                                                     >  2:703501/1‑140 (MQ=255)           agtgagtCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCg                                                                                                                     >  2:712449/1‑140 (MQ=255)           agtgaggCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCg                                                                                                                     >  2:348200/1‑140 (MQ=255)             tgagtCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGAc                                                                                                                   >  1:414737/1‑140 (MQ=255)                gtCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTgg                                                                                                                >  1:611077/1‑140 (MQ=255)                   ttCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCgg                                                                                                             >  1:277875/1‑140 (MQ=255)                   ttCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCgg                                                                                                             >  1:524752/1‑140 (MQ=255)                   ttCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCgg                                                                                                             >  1:426945/1‑140 (MQ=255)                     cGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGa                                                                                                           <  2:277875/140‑1 (MQ=255)                       cTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGaaa                                                                                                         <  1:712449/140‑1 (MQ=255)                         gTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACg                                                                                                       <  2:460587/140‑1 (MQ=255)                         gTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACg                                                                                                       <  2:124805/140‑1 (MQ=255)                           aaTCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAAAGcc                                                                                                     >  1:116657/1‑140 (MQ=255)                            aTCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCa                                                                                                    >  1:100854/1‑140 (MQ=255)                             tCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCaa                                                                                                   <  2:426945/140‑1 (MQ=255)                             tCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCa                                                                                                    <  2:783545/139‑1 (MQ=255)                             tCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCa                                                                                                    >  1:783545/1‑139 (MQ=255)                              cGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAAc                                                                                                  <  1:813540/140‑1 (MQ=255)                                  gcgcAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATaa                                                                                              >  1:441833/1‑140 (MQ=255)                                    gcAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAATGCCAACAAAAtt                                                                                            >  1:906682/1‑139 (MQ=255)                                        ggAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGtgat                                                                                        <  1:348200/140‑1 (MQ=255)                                          aGTCCGGATCCATATGCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGtgatga                                                                                      <  2:423460/140‑1 (MQ=255)                                             ccGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGc                                                                                   >  2:118705/1‑140 (MQ=255)                                                      tatCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGcggg                                                                          >  2:775268/1‑137 (MQ=255)                                                      tatCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGt                                                                          >  1:354616/1‑140 (MQ=255)                                                      tatCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGt                                                                          >  2:175834/1‑140 (MQ=255)                                                          cAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTcgc                                                                      >  1:507099/1‑140 (MQ=255)                                                             cTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGAc                                                                   >  2:179404/1‑140 (MQ=255)                                                             cTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGAc                                                                   >  2:162957/1‑140 (MQ=255)                                                             cTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAAAGCCCACATAATGTGATGACGCTGCGGCAGGTCGCGAc                                                                   >  2:721494/1‑140 (MQ=255)                                                               cgcgGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTc                                                                 >  1:542997/1‑140 (MQ=255)                                                               cgcgGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTc                                                                 <  1:162957/140‑1 (MQ=255)                                                                gcgGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTcc                                                                >  2:885250/1‑140 (MQ=255)                                                                 cgGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCa                                                               >  1:270668/1‑140 (MQ=255)                                                                 cgGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGGGATGACGCTGCGGCAGTTtgcggccccc                                                               >  2:133313/1‑134 (MQ=255)                                                                         cGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTaaca                                                       <  2:116657/140‑1 (MQ=255)                                                                         cGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTaaca                                                       <  1:118705/140‑1 (MQ=255)                                                                                    gCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGcc                                            <  1:323340/140‑1 (MQ=255)                                                                                     ccAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCa                                           >  1:850677/1‑140 (MQ=255)                                                                                      cAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAg                                          <  2:414737/140‑1 (MQ=255)                                                                                            gTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGctgctg                                    <  2:354616/140‑1 (MQ=255)                                                                                              ttGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTgcg                                  <  1:4846/140‑1 (MQ=255)                                                                                                            cAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGc                    <  1:179404/140‑1 (MQ=255)                                                                                                            cAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGc                    <  1:805965/140‑1 (MQ=255)                                                                                                            cAAGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGc                    <  1:133313/140‑1 (MQ=255)                                                                                                             aaGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGcc                   <  2:507099/140‑1 (MQ=255)                                                                                                              aGGGACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGccc                  <  1:537738/140‑1 (MQ=255)                                                                                                               gggACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCa                 <  2:478994/140‑1 (MQ=255)                                                                                                                ggACATCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCAt                <  1:721494/140‑1 (MQ=255)                                                                                                                    aTCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACTCCAACATAATGTCATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCttt            <  2:611077/140‑1 (MQ=255)                                                                                                                    aTCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCttt            >  1:899037/1‑140 (MQ=255)                                                                                                                    aTCGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGGAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCCTCttt            >  2:44386/1‑140 (MQ=255)                                                                                                                      cGACGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCCCGGTAACAAAGTTTTAGCCAGCTGCTGCGCCTCAACAAACCGCCCCTCTTTGa          >  2:900915/1‑140 (MQ=255)                                                                                                                        aCGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCTTTGATa        <  1:414752/140‑1 (MQ=255)                                                                                                                         cGGGCGCGGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCTTTGATaa       <  1:175834/140‑1 (MQ=255)                                                                                                                             cgcgGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCTTTGATAATGTc   <  2:270668/140‑1 (MQ=255)                                                                                                                              gcgGAAATGGCCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCTTTGATAATGTCg  >  1:887457/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   GGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGTTTGCTCCCGCTCACCAAGGGACATCGACGGGCGCGGAAATGACCGCTGTAATGTGCGACTGGCGGGAAACGCCAACATAATGTGATGACGCTGCGGCAGTTCGCGACTCCACGGTAACAACGTTTTAGCCAGCTGCTGCGCATCAACAATCCGCCCATCTTTGATAATGTCG  >  NC_000913/3522365‑3522629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑