Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,714,532	C→T	W621* (TGG→TGA)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,714,532	0	C	T	100.0%	173.8 / NA	50	W621* (TGG→TGA)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/25);  total (25/25)

CTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCT  >  NC_000913/3714397‑3714668                                                                                                                                        |                                                                                                                                         cTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTcaca                                                                                                                                      >  1:460030/1‑140 (MQ=255)   cACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACAtt                                                                                                                                    >  2:334163/1‑140 (MQ=255)     cGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGTCCTGCCATTCGTCCGGCTCCAGTCACATTgg                                                                                                                                  <  1:140591/140‑1 (MQ=255)     cGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTgg                                                                                                                                  <  2:907205/140‑1 (MQ=255)     cGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTgg                                                                                                                                  <  2:583685/140‑1 (MQ=255)     cGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTgg                                                                                                                                  >  2:127772/1‑140 (MQ=255)     cGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTgg                                                                                                                                  <  1:331481/140‑1 (MQ=255)     cGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTgg                                                                                                                                  <  1:307484/140‑1 (MQ=255)     cGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTCCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTgg                                                                                                                                  <  1:521978/140‑1 (MQ=255)        tttGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATg                                                                                                                               >  1:222061/1‑140 (MQ=255)          tGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGcc                                                                                                                             <  1:710926/140‑1 (MQ=255)                    tACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATc                                                                                                                   >  1:735748/1‑140 (MQ=255)                         ttCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGt                                                                                                              >  1:830920/1‑140 (MQ=255)                                agagAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTa                                                                                                       <  2:222061/140‑1 (MQ=255)                                  agaACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAAt                                                                                                     <  2:285049/140‑1 (MQ=255)                                           aTTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATAc                                                                                            <  2:735748/140‑1 (MQ=255)                                            ttCAGCTGGCTGTGCAGGCGGTGCGCCGGGTGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCCGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACg                                                                                           >  2:696252/1‑140 (MQ=255)                                            ttCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACg                                                                                           <  2:53537/140‑1 (MQ=255)                                            ttCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACg                                                                                           <  1:845900/140‑1 (MQ=255)                                            ttCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACg                                                                                           <  2:460030/140‑1 (MQ=255)                                              cAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCt                                                                                         >  1:738953/1‑140 (MQ=255)                                                        tgCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTTCCCTGCCATTCGTCCGGCCCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGAt                                                                               >  1:234919/1‑140 (MQ=255)                                                        tgCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGAt                                                                               <  1:127772/140‑1 (MQ=255)                                                        tgCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGAt                                                                               >  1:29033/1‑140 (MQ=255)                                                                  gcgcCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTg                                                                     >  1:693937/1‑140 (MQ=255)                                                                    gcCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGcc                                                                   >  1:644337/1‑140 (MQ=255)                                                                      cGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGc                                                                 <  2:830920/140‑1 (MQ=255)                                                                            ggCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCgg                                                           <  2:462170/140‑1 (MQ=255)                                                                                  aaGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCg                                                            >  2:384481/1‑133 (MQ=255)                                                                                  aaGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCg                                                            <  1:384481/133‑1 (MQ=255)                                                                                     tACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACg                                                  <  2:29033/140‑1 (MQ=255)                                                                                      aCCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTgg                                                              <  1:181909/127‑1 (MQ=255)                                                                                      aCCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTgg                                                              >  2:181909/1‑127 (MQ=255)                                                                                           cAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATgcg                                            <  1:334163/140‑1 (MQ=255)                                                                                               tGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAg                                        >  1:263344/1‑140 (MQ=255)                                                                                                 ttCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCg                                      >  2:104273/1‑140 (MQ=255)                                                                                                    tGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGAt                                   <  1:72754/140‑1 (MQ=255)                                                                                                      gTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATcg                                 >  2:438889/1‑140 (MQ=255)                                                                                                      gTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATcg                                 <  1:438889/140‑1 (MQ=255)                                                                                                           gCATTGCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCa                            >  1:599443/1‑140 (MQ=255)                                                                                                                gCCCTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAAt                       >  1:430869/1‑140 (MQ=255)                                                                                                                 cccTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATc                      >  2:280058/1‑140 (MQ=255)                                                                                                                 cccTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATc                      >  2:773236/1‑140 (MQ=255)                                                                                                                 cccTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAAC‑‑‑‑GCGCCAGCGGATCGCGGCAAAAATCagcg                  >  1:200686/1‑140 (MQ=255)                                                                                                                  ccTGCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAAC‑‑‑‑GCGCCAGCGGATCGCGGCAAAAATCagcga                 <  2:200686/140‑1 (MQ=255)                                                                                                                     gCCATTCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCagcg                  >  2:800836/1‑140 (MQ=255)                                                                                                                         ttCGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCagcgaagc              >  2:253388/1‑140 (MQ=255)                                                                                                                           cGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCagcgaagcga            >  1:376076/1‑140 (MQ=255)                                                                                                                           cGTCCGGCTCCAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCagcgaagcga            >  1:471981/1‑140 (MQ=255)                                                                                                                                     cAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACcgct  <  2:471981/140‑1 (MQ=255)                                                                                                                                     cAGTCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACcgct  <  2:430869/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CTCACGATTTGCCACCGCGTACAATTCGCGCAGAGAACTGTAATTCAGCTGGCTGTGCAGGCGGTGCGCCGGATGGGCAGAAAGTACCTGCAACTGTTCTGGTTCGGCATTGCCCTGCCATTCGTCCGGCTCCAGCCACATTGGATGCCCAGGGCAATCCGGGTAACCGTAATCGGCAATACGCTGTGAGAAGATTTCAATCTTGCCGCTGGCGGTTTTTAACGGATGCGCCAGCGGATCGCGGCAAAAATCAGCGAAGCGAATAAACCGCT  >  NC_000913/3714397‑3714668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑