Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,784,538	G→A	intergenic (‑96/+46)	grxC ← / ← yibN	glutaredoxin 3/putative sulfurtransferase YibN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,784,538	0	G	A	100.0%	187.0 / NA	53	intergenic (‑96/+46)	grxC/yibN	glutaredoxin 3/putative sulfurtransferase YibN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/25);  total (28/25)

GGCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGGCCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTC  >  NC_000913/3784406‑3784671                                                                                                                                         |                                                                                                                                      ggCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAAtt                                                                                                                                >  2:726066/1‑140 (MQ=255)  gCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTg                                                                                                                               >  2:875856/1‑140 (MQ=255)  gCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTg                                                                                                                               <  1:495410/140‑1 (MQ=255)   cAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGc                                                                                                                              >  1:553993/1‑140 (MQ=255)        ttCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTa                                                                                                                         <  2:193853/140‑1 (MQ=255)             tGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGc                                                                                                                    <  1:875856/140‑1 (MQ=255)              ggTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGcc                                                                                                                   <  1:476473/140‑1 (MQ=255)               gTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCt                                                                                                                  <  2:656298/140‑1 (MQ=255)               gTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCt                                                                                                                  <  1:860590/140‑1 (MQ=255)                 ataGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGc                                                                                                                >  2:355172/1‑140 (MQ=255)                 ataGATTTCAACATCGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGc                                                                                                                <  2:567256/140‑1 (MQ=255)                  taGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGca                                                                                                               <  1:453176/140‑1 (MQ=255)                     aTTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAACTG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGcatca                                                                                                            >  2:641507/1‑140 (MQ=255)                     aTTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGcatca                                                                                                            >  1:529014/1‑140 (MQ=255)                     aTTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGcatca                                                                                                            >  2:559864/1‑140 (MQ=255)                          aaCATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGaaa                                                                                                       >  1:707400/1‑140 (MQ=255)                             aTTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgac                                                                                                    <  2:553993/140‑1 (MQ=255)                             aTTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgac                                                                                                    <  1:726066/140‑1 (MQ=255)                               tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATGGAGTGAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAg                                                                                                      >  1:826709/1‑140 (MQ=255)                               tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacga                                                                                                  <  1:559864/140‑1 (MQ=255)                               tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacga                                                                                                  >  1:672841/1‑140 (MQ=255)                               tGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacga                                                                                                  >  1:75350/1‑140 (MQ=255)                                ggCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAgacgac                                                                                                 <  2:672841/140‑1 (MQ=255)                                 gCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACa                                                                                                >  1:647013/1‑140 (MQ=255)                                                 tGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCcg                                                                                >  1:175201/1‑140 (MQ=255)                                                 tGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCcg                                                                                <  2:529014/140‑1 (MQ=255)                                                  gTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCcgc                                                                               >  2:423916/1‑140 (MQ=255)                                                             cTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGc                                                                    >  1:547773/1‑140 (MQ=255)                                                             cTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGc                                                                    <  2:701588/140‑1 (MQ=255)                                                                      cATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCg                                                           <  2:647013/140‑1 (MQ=255)                                                                           tGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGccagc                                                      <  1:643624/140‑1 (MQ=255)                                                                                      agcaAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  2:62744/140‑1 (MQ=255)                                                                                      agcaAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  1:423916/140‑1 (MQ=255)                                                                                                   gAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCcttctt                                  >  1:652563/1‑140 (MQ=255)                                                                                                     gtgtCCCCAGAAGCTTCTAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTc                                <  2:826709/140‑1 (MQ=255)                                                                                                     gtgtCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTc                                <  1:641507/140‑1 (MQ=255)                                                                                                      tgtCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCa                               >  1:435583/1‑140 (MQ=255)                                                                                                      tgtCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCa                               <  1:355172/140‑1 (MQ=255)                                                                                                        tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           >  1:910063/1‑126 (MQ=255)                                                                                                        tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  2:910063/126‑1 (MQ=255)                                                                                                        tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGc                             >  1:853173/1‑140 (MQ=255)                                                                                                        tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGc                             >  1:702382/1‑140 (MQ=255)                                                                                                        tCCCCAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGc                             >  1:685721/1‑140 (MQ=255)                                                                                                           ccAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           <  2:421388/123‑1 (MQ=255)                                                                                                           ccAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGa                                           >  1:421388/1‑123 (MQ=255)                                                                                                           ccAGAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACg                          <  1:583028/140‑1 (MQ=255)                                                                                                              gAAGCTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAAt                       >  2:127906/1‑140 (MQ=255)                                                                                                                  cTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACtt                   >  2:323780/1‑140 (MQ=255)                                                                                                                  cTTATAACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACtt                   >  1:478287/1‑140 (MQ=255)                                                                                                                    tataACAATAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTgc                 >  2:8334/1‑140 (MQ=255)                                                                                                                           aTAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAAc          <  2:707400/140‑1 (MQ=255)                                                                                                                            tAAGTCGCTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAAcc         >  1:240164/1‑140 (MQ=255)                                                                                                                                   cTGGGGACCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTc  >  2:663170/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      GGCAGGTTTCTTTGGTATAGATTTCAACATTGGCCATAAATTAGCTCCTGTTTTTTTACCCTGAAATTTCATGTTGCAGGGCGGCAGCAAAATG‑‑‑‑GAGTGTCCCCAGAAGCTTATAACAATAAGTCGCTGGGGGCCTAATTGCAGCTAACGGCCTGCATCATGAAAGACGACAGGTAAATTATTTGCCGCGCACCAAAGGCAAGTTTTCGCCAGCCCAGCCAGCGACGCCTTCTTTCAGCACGAATACTTGCGCAAAACCAGCTTTC  >  NC_000913/3784406‑3784671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑