Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,077,754	C→T	G102G (GGC→GGT)	yiiD →	putative acetyltransferase YiiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,077,754	0	C	T	100.0%	161.3 / NA	49	G102G (GGC→GGT)	yiiD	putative acetyltransferase YiiD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/16);  total (33/16)

TCACCAGATGGTCGTCGACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCAC  >  NC_000913/4077628‑4077882                                                                                                                               |                                                                                                                                 tCACCAGATGGTCGTCGACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGa                                                                                                                     <  1:398545/140‑1 (MQ=255) tCACCAGATGGTCGTCGACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGa                                                                                                                     <  2:314095/140‑1 (MQ=255)        aTGGTCGTCGACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGa                                                                                                                     <  2:287356/133‑1 (MQ=255)        aTGGTCGTCGACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGa                                                                                                                     >  1:287356/1‑133 (MQ=255)           gtcgtcGACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGa                                                                                                           >  2:467102/1‑140 (MQ=255)            tcgtcgACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAg                                                                                                          >  1:157241/1‑140 (MQ=255)                      gCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGgcgc                                                                                                >  2:339179/1‑140 (MQ=255)                            tAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGTGCGTCAgg                                                                                          <  1:831017/140‑1 (MQ=255)                                    tggCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGtt                                                                                  >  2:505977/1‑140 (MQ=255)                                    tggCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGtt                                                                                  >  1:799425/1‑140 (MQ=255)                                    tggCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGtt                                                                                  >  1:666171/1‑140 (MQ=255)                                    tggCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGtt                                                                                  >  2:101215/1‑140 (MQ=255)                                                            aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAgc                                                                <  2:334279/134‑1 (MQ=255)                                                            aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAgc                                                                >  1:334279/1‑134 (MQ=255)                                                            aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                          >  2:220215/1‑140 (MQ=255)                                                            aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                          >  1:647275/1‑140 (MQ=255)                                                            aTGCCGACAATGAAGCGTCCATTCGCCTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                          >  2:557621/1‑140 (MQ=255)                                                             tGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                                                         >  1:805164/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  1:128086/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  2:173609/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  1:786553/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  2:667592/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  2:380756/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  1:761109/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  1:300729/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  1:709877/1‑140 (MQ=255)                                                               ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                       >  1:630551/1‑140 (MQ=255)                                                                   cAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGAcg                                                   <  1:380756/140‑1 (MQ=255)                                                                   cAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGAcg                                                   <  1:173609/140‑1 (MQ=255)                                                                      tGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCgg                                                >  1:196073/1‑140 (MQ=255)                                                                         aGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTgg                                             >  1:314785/1‑140 (MQ=255)                                                                         aGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTgg                                             >  1:311450/1‑140 (MQ=255)                                                                             tCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGtt                                         >  1:878269/1‑140 (MQ=255)                                                                              ccATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGttt                                        >  2:416831/1‑140 (MQ=255)                                                                                aTTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGttttt                                      >  2:691684/1‑140 (MQ=255)                                                                                  tCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCg                                    >  1:772667/1‑140 (MQ=255)                                                                                         aTGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCt                             <  1:453146/140‑1 (MQ=255)                                                                                          tGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTg                            >  1:196614/1‑140 (MQ=255)                                                                                            gCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                          <  1:467102/140‑1 (MQ=255)                                                                                            gCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                          <  2:196073/140‑1 (MQ=255)                                                                                            gCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                          <  2:585431/140‑1 (MQ=255)                                                                                                ttCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGttt                      >  1:562086/1‑140 (MQ=255)                                                                                                ttCATCCCGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGttt                      >  1:789422/1‑140 (MQ=255)                                                                                                     cccGACGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTaa                 <  1:220215/140‑1 (MQ=255)                                                                                                         aCGTGCAGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAg             <  2:314785/140‑1 (MQ=255)                                                                                                               aGGACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGaa       <  1:758279/140‑1 (MQ=255)                                                                                                                ggACAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGaaa      >  1:432330/1‑140 (MQ=255)                                                                                                                   cAAAGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATca   <  2:772667/140‑1 (MQ=255)                                                                                                                    aaaGGGTTAGGTACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATcac  <  2:196614/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                 TCACCAGATGGTCGTCGACGAGCAGGGTAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCAC  >  NC_000913/4077628‑4077882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑