Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,157,174	C→T	R109C (CGT→TGT)	argH →	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,157,174	0	C	T	100.0%	151.8 / NA	43	R109C (CGT→TGT)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/23);  total (20/23)

TGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGT  >  NC_000913/4157040‑4157308                                                                                                                                       |                                                                                                                                       tgtTGCTGGAAGATGTTCGCGCCAGG‑‑‑CAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAAttat                                                                                                                                <  2:876869/140‑4 (MQ=255)    tGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGAt                                                                                                                                <  2:89170/140‑1 (MQ=255)    tGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGAt                                                                                                                                >  2:720816/1‑140 (MQ=255)    tGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGAt                                                                                                                                <  2:257748/140‑1 (MQ=255)    tGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGAt                                                                                                                                <  1:4695/140‑1 (MQ=255)    tGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGAt                                                                                                                                <  1:553300/140‑1 (MQ=255)              tGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGa                                                                                                                      <  2:261719/140‑1 (MQ=255)                         ggCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGaaa                                                                                                           <  1:605682/140‑1 (MQ=255)                            cacaACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACtg                                                                                                        <  2:595714/140‑1 (MQ=255)                             acaactAATCCTTGGAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACtgt                                                                                                       <  2:816528/140‑1 (MQ=255)                                 caaATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTgtggtg                                                                                                   <  1:88311/140‑1 (MQ=255)                                    aTCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCaa                                                                                                >  1:215660/1‑140 (MQ=255)                                               cGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTa                                                                                     <  1:845792/140‑1 (MQ=255)                                                 aCGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGc                                                                                   <  2:270902/140‑1 (MQ=255)                                                    ccGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAg                                                                                >  1:841420/1‑140 (MQ=255)                                                                 tAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTa                                                                   >  2:256895/1‑140 (MQ=255)                                                                  aGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTaa                                                                  >  1:429596/1‑140 (MQ=255)                                                                   gCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAAc                                                                 >  1:204509/1‑140 (MQ=255)                                                                    cTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAAcc                                                                >  1:613341/1‑140 (MQ=255)                                                                      gggTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCgg                                                              >  1:530249/1‑140 (MQ=255)                                                                       ggTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGc                                                             >  1:495824/1‑140 (MQ=255)                                                                       ggTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGc                                                             >  2:500945/1‑140 (MQ=255)                                                                              ggCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCa                                                      >  1:575970/1‑140 (MQ=255)                                                                              ggCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCa                                                      >  2:403622/1‑140 (MQ=255)                                                                                   aCTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCgg                                                 >  1:411616/1‑140 (MQ=255)                                                                                     tGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGgcg                                               <  2:43195/140‑1 (MQ=255)                                                                                      gATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGgcgc                                              >  1:650155/1‑140 (MQ=255)                                                                                      gATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGgcgc                                              >  1:330447/1‑140 (MQ=255)                                                                                                 tGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAAcc                                   <  1:403622/140‑1 (MQ=255)                                                                                                 tGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAAcc                                   >  1:319311/1‑140 (MQ=255)                                                                                                  gggCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCg                                  <  2:204509/140‑1 (MQ=255)                                                                                                   ggCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGc                                 <  1:27555/140‑1 (MQ=255)                                                                                                       aGTTAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACaa                             <  2:319311/140‑1 (MQ=255)                                                                                                        gTTAGGCAAAAAGCTGCATTCCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACaaa                            <  1:663495/140‑1 (MQ=255)                                                                                                         ttAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACaaaa                           >  2:810331/1‑140 (MQ=255)                                                                                                         ttAGGCAAAAAGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACaaaa                           >  2:266470/1‑140 (MQ=255)                                                                                                                   aGCTGCATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGAc                 <  1:810331/140‑1 (MQ=255)                                                                                                                        cATACCGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGt            <  2:530249/140‑1 (MQ=255)                                                                                                                            ccGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATg        <  2:337850/140‑1 (MQ=255)                                                                                                                            ccGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATg        <  1:500945/140‑1 (MQ=255)                                                                                                                             cGGGCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGc       >  2:523061/1‑140 (MQ=255)                                                                                                                               ggCGTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCa     >  2:294776/1‑140 (MQ=255)                                                                                                                                  gTAGCTGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGt  <  1:906232/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TGTTGCTGGAAGATGTTCGCGCCAGGCCACAACAAATCCTTGAAAGCGACGCCGAAGATATCCATAGCTGGGTGGAAGGCAAACTGATCGACAAAGTGGGCCAGTTAGGCAAAAAGCTGCATACCGGGCGTAGCCGTAATGATCAGGTAGCGACTGACCTGAAACTGTGGTGCAAAGATACCGTTAGCGAGTTACTGACGGCTAACCGGCAGCTGCAATCGGCGCTGGTGGAAACCGCACAAAACAATCAGGACGCGGTAATGCCAGGT  >  NC_000913/4157040‑4157308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑