Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,178,857	C→T	G137G (GGC→GGT)	rplK →	50S ribosomal subunit protein L11

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,178,857	0	C	T	100.0%	169.9 / NA	48	G137G (GGC→GGT)	rplK	50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

AACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAA  >  NC_000913/4178726‑4178989                                                                                                                                    |                                                                                                                                     aaCAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGt                                                                                                                              >  1:784740/1‑140 (MQ=255)     aagacaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTggag                                                                                                                          <  1:902018/140‑1 (MQ=255)          aaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTa                                                                                                                     <  2:240770/140‑1 (MQ=255)             gTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGTCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGa                                                                                                                  >  2:873537/1‑140 (MQ=255)                 gTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATg                                                                                                              >  2:886819/1‑140 (MQ=255)                     aaTTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTa                                                                                                          <  1:479490/140‑1 (MQ=255)                     aaTTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTa                                                                                                          >  1:780090/1‑140 (MQ=255)                       tttCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTaaa                                                                                                        <  1:184800/140‑1 (MQ=255)                             gcgcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGTCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGAcc                                                                                                  <  1:873537/140‑1 (MQ=255)                                cTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAg                                                                                               <  2:164215/140‑1 (MQ=255)                                cTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAg                                                                                               <  1:71866/140‑1 (MQ=255)                                      tGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATg                                                                                         <  1:886819/140‑1 (MQ=255)                                               tCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTTACCAAGCGCATGCGTGTTATc                                                                                >  1:502520/1‑140 (MQ=255)                                                            aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                <  1:269478/127‑1 (MQ=255)                                                            aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                <  1:298522/127‑1 (MQ=255)                                                            aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                >  2:298522/1‑127 (MQ=255)                                                            aGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATc                                                                                >  2:269478/1‑127 (MQ=255)                                                             gCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTTCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGa                                                                  <  1:104429/140‑1 (MQ=255)                                                             gCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGa                                                                  <  1:461966/140‑1 (MQ=255)                                                                 ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATg                                                                >  1:846392/1‑138 (MQ=255)                                                                 ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATg                                                                <  2:846392/138‑1 (MQ=255)                                                                 ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCa                                                              >  2:776718/1‑140 (MQ=255)                                                                 ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCa                                                              >  2:305566/1‑140 (MQ=255)                                                                 ccGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCACGAGAAAGTTGATGCa                                                              >  2:121286/1‑140 (MQ=255)                                                                  cGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTTGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCaa                                                             <  2:202561/140‑1 (MQ=255)                                                                       tGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCaaa                                                        <  2:784740/140‑1 (MQ=255)                                                                       tGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCaaa                                                        <  1:126063/140‑1 (MQ=255)                                                                            ggTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTa                                                   <  2:870441/140‑1 (MQ=255)                                                                             gTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTAc                                                  <  1:790207/140‑1 (MQ=255)                                                                                ccGACATTGAAGCGATTACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGAc                                               >  2:87892/1‑140 (MQ=255)                                                                                ccGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGAc                                               >  1:340619/1‑140 (MQ=255)                                                                                ccGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGAc                                               <  2:340619/140‑1 (MQ=255)                                                                                        gAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGa                                       <  1:305566/140‑1 (MQ=255)                                                                                              aTGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTAt                                 >  1:76183/1‑140 (MQ=255)                                                                                               tGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATc                                >  1:129425/1‑140 (MQ=255)                                                                                               tGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATc                                >  2:814042/1‑140 (MQ=255)                                                                                                 aCTCGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGc                              <  2:604727/140‑1 (MQ=255)                                                                                                    cGCTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCAct                           <  1:87892/140‑1 (MQ=255)                                                                                                      cTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCActgc                         >  2:492077/1‑140 (MQ=255)                                                                                                      cTCCATCGAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCActgc                         >  2:741694/1‑140 (MQ=255)                                                                                                             gAAGGTACTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAaga                  >  1:290851/1‑140 (MQ=255)                                                                                                                   aCTGCACGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTTACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGc            <  2:502520/140‑1 (MQ=255)                                                                                                                         cGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGc            <  2:667759/134‑1 (MQ=255)                                                                                                                         cGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGc            >  1:667759/1‑134 (MQ=255)                                                                                                                         cGTTCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGc      >  2:614498/1‑140 (MQ=255)                                                                                                                           ttCCATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTa    >  1:34981/1‑140 (MQ=255)                                                                                                                             ccATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTaaa  >  2:340643/1‑140 (MQ=255)                                                                                                                             ccATGGGTCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTaaa  <  1:121286/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                     AACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCACGTTCCATGGGCCTGGTAGTGGAGGACTAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAA  >  NC_000913/4178726‑4178989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑