Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,113	G→C	G212A (GGT→GCT)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,113	0	G	C	100.0%	199.7 / NA	54	G212A (GGT→GCT)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/32);  total (22/32)

AACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTG  >  NC_000913/4278977‑4279244                                                                                                                                         |                                                                                                                                      aaCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGGTCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTAt                                                                                                                                    <  1:87829/140‑1 (MQ=255)       cTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAAt                                                                                                                              >  1:133092/1‑140 (MQ=255)         tGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTg                                                                                                                            <  2:764414/140‑1 (MQ=255)         tGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTg                                                                                                                            <  2:66593/140‑1 (MQ=255)               tCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGtt                                                                                                                      <  2:876933/140‑1 (MQ=255)                 tGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTg                                                                                                                    >  1:165843/1‑140 (MQ=255)                 tGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTg                                                                                                                    <  1:3661/140‑1 (MQ=255)                        tGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCg                                                                                                             >  2:4921/1‑140 (MQ=255)                           gcTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATc                                                                                                          <  1:574813/140‑1 (MQ=255)                             tGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCt                                                                                                        >  2:858926/1‑140 (MQ=255)                                            ccTTCCCGGCGATTATGTCGCTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGCAAGTCCCTGGTGGCATTCTTCTGACCATTATCGCTATCTCAATTGTCGGTTTTATTTTCCATCCTAA‑‑CGTCCCTTTCTcc                                                                                       >  2:887049/1‑140 (MQ=255)                                             cTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCg                                                                                      <  2:535873/140‑1 (MQ=255)                                                  cGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGtt                                                                                 <  1:252373/140‑1 (MQ=255)                                                       aTTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGc                                                                            >  1:879111/1‑140 (MQ=255)                                                         tATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGCGTCCATTTCTCCGGCGTTTTCGc                                                                            <  2:348457/140‑1 (MQ=255)                                                         tATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCa                                                                          <  2:552271/140‑1 (MQ=255)                                                         tATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCa                                                                          <  2:471851/140‑1 (MQ=255)                                                          aTGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCAt                                                                         >  1:339517/1‑140 (MQ=255)                                                            gTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGc                                                                       >  1:369990/1‑140 (MQ=255)                                                            gTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGc                                                                       <  2:11649/140‑1 (MQ=255)                                                             tCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGcc                                                                      <  2:226333/140‑1 (MQ=255)                                                                 tGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCa                                                                  >  2:436654/1‑140 (MQ=255)                                                                 tGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCa                                                                  >  2:401249/1‑140 (MQ=255)                                                                      ggTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGcc                                                                           <  2:7652/126‑1 (MQ=255)                                                                      ggTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGcc                                                                           >  1:7652/1‑126 (MQ=255)                                                                       gTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGc                                                            >  2:227549/1‑140 (MQ=255)                                                                           ggCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCAtt                                                                >  1:608854/1‑132 (MQ=255)                                                                           ggCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCAtt                                                                <  2:608854/132‑1 (MQ=255)                                                                           ggCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATg                                                        >  2:804266/1‑140 (MQ=255)                                                                             cGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGaa                                                      >  1:492675/1‑140 (MQ=255)                                                                                  atcatcGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACgg                                                 >  1:749588/1‑140 (MQ=255)                                                                                      tcGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAAt                                             >  1:253482/1‑140 (MQ=255)                                                                                       cGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAAtt                                            <  1:4921/140‑1 (MQ=255)                                                                                       cGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAAtt                                            <  1:876086/140‑1 (MQ=255)                                                                                          ccTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCAc                                         >  1:355673/1‑140 (MQ=255)                                                                                            tGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTg                                       >  1:885240/1‑140 (MQ=255)                                                                                            tGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTg                                       >  1:770889/1‑140 (MQ=255)                                                                                              gAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGAt                                     >  2:735391/1‑140 (MQ=255)                                                                                                aaaaCTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTg                                   <  2:770889/140‑1 (MQ=255)                                                                                                  aaCTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGc                                 <  2:33986/140‑1 (MQ=255)                                                                                                     tGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGc                              <  1:478698/140‑1 (MQ=255)                                                                                                     tGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGc                              <  1:369710/140‑1 (MQ=255)                                                                                                      gAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGcc                             <  1:121264/140‑1 (MQ=255)                                                                                                        aaGTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTg                           <  1:713280/140‑1 (MQ=255)                                                                                                          gTCCCTGGTGGCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGa                         <  2:369990/140‑1 (MQ=255)                                                                                                                   ggCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATggg                <  1:227549/140‑1 (MQ=255)                                                                                                                    gCATTCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGgc               <  2:339517/140‑1 (MQ=255)                                                                                                                       ttCTGCTGACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGgcgcg            >  1:111201/1‑140 (MQ=255)                                                                                                                         ctgctgACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCt          <  2:492675/140‑1 (MQ=255)                                                                                                                         ctgctgACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCt          <  1:858926/140‑1 (MQ=255)                                                                                                                         ctgctgACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCt          <  1:213821/140‑1 (MQ=255)                                                                                                                              gACCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATc     <  1:804266/140‑1 (MQ=255)                                                                                                                               aCCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGaa       <  1:676143/137‑1 (MQ=255)                                                                                                                               aCCATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGaa       >  2:676143/1‑137 (MQ=255)                                                                                                                                 cATTATCGCTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTg  <  2:39435/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAA‑‑CGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTG  >  NC_000913/4278977‑4279244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑