Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,282,493	C→T	E195K (GAA→AAA)	yjcF ←	pentapeptide repeat‑containing protein YjcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,282,493	0	C	T	100.0%	247.5 / NA	69	E195K (GAA→AAA)	yjcF	pentapeptide repeat‑containing protein YjcF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/32);  total (37/32)

ATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTT  >  NC_000913/4282362‑4282613                                                                                                                                    |                                                                                                                         atTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCAATATgt                                                                                                                  >  2:911618/1‑139 (MQ=255)  ttGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGc                                                                                                                 <  1:783228/140‑1 (MQ=255)     gCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTAATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGaa                                                                                                              >  2:501383/1‑140 (MQ=255)          aTTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTaa                                                                                                         >  1:610671/1‑140 (MQ=255)             cATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATTAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAAt                                                                                                      <  2:340088/140‑1 (MQ=255)             cATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAGAAAGTATTTTTTTCATTATGGCGAAATTAAAAt                                                                                                      <  1:172092/140‑1 (MQ=255)                tgttgCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCg                                                                                                   >  2:440535/1‑140 (MQ=255)                tgttgCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCg                                                                                                   >  2:758804/1‑140 (MQ=255)                tgttgCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGACATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCg                                                                                                   >  1:327533/1‑140 (MQ=255)                  ttgCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGtt                                                                                                 <  1:390820/140‑1 (MQ=255)                  ttgCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGtt                                                                                                 <  1:568517/140‑1 (MQ=255)                    gCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGtttt                                                                                               <  1:284029/140‑1 (MQ=255)                    gCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGtttt                                                                                               <  1:205355/140‑1 (MQ=255)                    gCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGtttt                                                                                               <  1:552314/140‑1 (MQ=255)                     cATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTta                                                                                              >  2:712762/1‑140 (MQ=255)                     cATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTta                                                                                              >  2:594915/1‑140 (MQ=255)                     cATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTta                                                                                              >  2:297814/1‑140 (MQ=255)                     cATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAACGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTta                                                                                              >  1:821044/1‑140 (MQ=255)                       ttGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTtata                                                                                            >  1:22558/1‑140 (MQ=255)                          tAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAAt                                                                                                      >  1:912987/1‑127 (MQ=255)                          tAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAAt                                                                                                      <  2:912987/127‑1 (MQ=255)                              aTTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGACATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTa                                                                                     <  2:327533/140‑1 (MQ=255)                              aTTGAACATGCATAGCGGAACATGAATTTAGAGTTACGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTa                                                                                     <  1:256002/140‑1 (MQ=255)                                  aaCATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGtttt                                                                                               >  2:527099/1‑126 (MQ=255)                                  aaCATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGtttt                                                                                               <  1:527099/126‑1 (MQ=255)                                  aaCATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGt                                                                                 >  2:538074/1‑140 (MQ=255)                                  aaCATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGt                                                                                 >  1:912871/1‑140 (MQ=255)                                  aaCATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGt                                                                                 >  1:259321/1‑140 (MQ=255)                                           aGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGaaaaa                                                                        <  2:345613/140‑1 (MQ=255)                                            gCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAAt                                                                       >  1:765536/1‑140 (MQ=255)                                             cGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATa                                                                      <  2:259321/140‑1 (MQ=255)                                             cGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATa                                                                      >  2:215498/1‑140 (MQ=255)                                             cGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATa                                                                      <  1:661001/140‑1 (MQ=255)                                             cGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATa                                                                      <  2:610671/140‑1 (MQ=255)                                               gAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGc                                                                    >  2:783188/1‑140 (MQ=255)                                                 aCATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGt                                                                  >  2:145811/1‑140 (MQ=255)                                                  cATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATa                                                                      >  2:543617/1‑135 (MQ=255)                                                  cATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATa                                                                      <  1:543617/135‑1 (MQ=255)                                                    tGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGtttt                                                               <  2:506009/140‑1 (MQ=255)                                                     gAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTg                                                              >  2:920822/1‑140 (MQ=255)                                                     gAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTg                                                              >  1:139945/1‑140 (MQ=255)                                                       aTTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTa                                                            >  1:405603/1‑140 (MQ=255)                                                       aTTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTa                                                            >  1:333193/1‑140 (MQ=255)                                                          tAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTc                                                         >  2:384637/1‑140 (MQ=255)                                                           agagTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCt                                                        <  1:297814/140‑1 (MQ=255)                                                            gagTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTg                                                              <  1:47077/133‑1 (MQ=255)                                                            gagTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTg                                                              >  2:47077/1‑133 (MQ=255)                                                                tCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAAc                                                   >  2:740430/1‑140 (MQ=255)                                                                 ccGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAAcc                                                  >  1:665707/1‑140 (MQ=255)                                                                              ttATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGaa                                     <  1:833815/140‑1 (MQ=255)                                                                              ttATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGaa                                     <  2:22558/140‑1 (MQ=255)                                                                                  cTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTgg                                 >  1:230285/1‑140 (MQ=255)                                                                                  cTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTCTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTgg                                 >  1:582209/1‑140 (MQ=255)                                                                                    gAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGAt                               <  1:384637/140‑1 (MQ=255)                                                                                      gCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATaa                             >  2:70167/1‑140 (MQ=255)                                                                                      gCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATaa                             >  2:286948/1‑140 (MQ=255)                                                                                            tttGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTc                       <  1:401754/140‑1 (MQ=255)                                                                                               gAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATc                    >  2:414618/1‑140 (MQ=255)                                                                                                  tCATCGAGAATACAATTCTCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTCGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCttt                 <  2:821044/140‑1 (MQ=255)                                                                                                     tCGAGAATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAAt              <  1:538074/140‑1 (MQ=255)                                                                                                       gagaATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCa            <  1:594915/140‑1 (MQ=255)                                                                                                       gagaATACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCa            <  1:215498/140‑1 (MQ=255)                                                                                                            tACAATTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATa       <  1:712762/140‑1 (MQ=255)                                                                                                                aTTATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCtt   <  2:230285/140‑1 (MQ=255)                                                                                                                 ttATCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCttt  >  1:212682/1‑140 (MQ=255)                                                                                                                   aTCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGAt        <  2:734802/132‑1 (MQ=255)                                                                                                                   aTCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGAt        >  1:734802/1‑132 (MQ=255)                                                                                                                    tCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCtt   <  1:269100/136‑1 (MQ=255)                                                                                                                    tCAAAAAGTATTTTTTTCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCtt   >  2:269100/1‑136 (MQ=255)                                                                                                                                    |                                                                                                                         ATTGGCTCTATTCATTGTTGCATTGTAGAATTGAACATGCATAGCGGAACATGAATTTAGAGTTCCGTCAGTCATTTTTATCTGAGCGAAATTTGAGTCATCGAGAATACAATTATCAAAAAGTATTTTTTCCATTATGGCGAAATTAAAATTCGTTTTATACATATTACAGTCCTGAAAAATAGCGTTTTGTAGTCTGCAACCTGCAAAATTCGAATTGGATAAATCTTCATCTTTAATCACGATACCTTT  >  NC_000913/4282362‑4282613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑