Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,412,691	T→C	V102A (GTT→GCT)	yjfM →	DUF1190 domain‑containing protein YjfM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,412,691	0	T	C	100.0%	197.2 / NA	54	V102A (GTT→GCT)	yjfM	DUF1190 domain‑containing protein YjfM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/26);  total (28/26)

GCGATAGTAGCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTA  >  NC_000913/4412556‑4412810                                                                                                                                        |                                                                                                                        gCGATAGTAGCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCg                                                                                                                     <  2:828390/140‑1 (MQ=255)    aTAGTAGCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCgaag                                                                                                                  >  2:889409/1‑140 (MQ=255)    aTAGTAGCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCgaag                                                                                                                  >  1:32499/1‑140 (MQ=255)    aTAGTAGCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCACTTTATGCCGATGCTCCgaag                                                                                                                  >  1:321460/1‑140 (MQ=255)      agtagCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCgaagaa                                                                                                                >  2:225517/1‑140 (MQ=255)      agtagCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCgaagaa                                                                                                                >  1:918475/1‑140 (MQ=255)          gCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATg                                                                                                            >  2:129129/1‑140 (MQ=255)           cGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGa                                                                                                           <  1:129129/140‑1 (MQ=255)           cGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGa                                                                                                           <  2:499958/140‑1 (MQ=255)           cGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGa                                                                                                           <  2:97868/140‑1 (MQ=255)             aTGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACt                                                                                                         >  1:860788/1‑140 (MQ=255)               gTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTca                                                                                                       >  2:818618/1‑140 (MQ=255)               gTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATG‑CGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTcag                                                                                                      >  1:308248/1‑140 (MQ=255)                     aaCGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGaa                                                                                                 <  1:225517/140‑1 (MQ=255)                          cggcgacggAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTc                                                                                            >  2:475786/1‑140 (MQ=255)                           ggcgacggAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGACGATGCTCCGAAGAATATGACTCAGCAGAACTGTCa                                                                                           <  1:610562/140‑1 (MQ=255)                             cgacggAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGt                                                                                         <  1:619459/140‑1 (MQ=255)                               acggAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCt                                                                                       <  2:82615/140‑1 (MQ=255)                                                    tGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCtactac                                                                  >  1:392363/1‑140 (MQ=255)                                                    tGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCtactac                                                                  >  1:151743/1‑140 (MQ=255)                                                      cAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGa                                                                <  2:918475/140‑1 (MQ=255)                                                      cAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGa                                                                <  2:673143/140‑1 (MQ=255)                                                      cAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGa                                                                <  1:818618/140‑1 (MQ=255)                                                      cAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGa                                                                <  1:889409/140‑1 (MQ=255)                                                           ttGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATg                                                           >  2:56566/1‑140 (MQ=255)                                                           ttGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATg                                                           >  2:23939/1‑140 (MQ=255)                                                              tATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTg                                                        >  1:278472/1‑140 (MQ=255)                                                              tATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTg                                                        >  2:212025/1‑140 (MQ=255)                                                              tATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTg                                                        >  1:202227/1‑140 (MQ=255)                                                              tATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTg                                                        >  2:472425/1‑140 (MQ=255)                                                                ttGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGaa                                                      <  2:256275/140‑1 (MQ=255)                                                                ttGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGaa                                                      <  2:321460/140‑1 (MQ=255)                                                                 tgatgaCGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAAc                                                     <  2:278472/140‑1 (MQ=255)                                                                   atgaCGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACag                                                   >  2:155691/1‑140 (MQ=255)                                                                     gaCGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGa                                                       <  1:808564/134‑1 (MQ=255)                                                                     gaCGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGa                                                       >  2:808564/1‑134 (MQ=255)                                                                                  ccGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTc                                    >  1:726131/1‑140 (MQ=255)                                                                                      cATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGttt                                >  2:26968/1‑140 (MQ=255)                                                                                            cgcgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGAt                          <  2:392363/140‑1 (MQ=255)                                                                                                  tGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGt                    >  1:52332/1‑140 (MQ=255)                                                                                                   ggCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGtt                   <  2:872349/140‑1 (MQ=255)                                                                                                   ggCTGGAACAACGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGtt                   <  1:56566/140‑1 (MQ=255)                                                                                                           caacGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAg                <  1:149882/135‑1 (MQ=255)                                                                                                           caacGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAg                >  2:149882/1‑135 (MQ=255)                                                                                                            aacGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGt          <  2:425536/140‑1 (MQ=255)                                                                                                            aacGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGt          <  1:320476/140‑1 (MQ=255)                                                                                                            aacGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGt          <  1:212025/140‑1 (MQ=255)                                                                                                              cGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGt          >  1:667696/1‑138 (MQ=255)                                                                                                              cGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTa        >  2:633173/1‑140 (MQ=255)                                                                                                              cGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTa        <  2:32499/140‑1 (MQ=255)                                                                                                              cGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTa        >  2:172572/1‑140 (MQ=255)                                                                                                              cGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTa        >  1:424717/1‑140 (MQ=255)                                                                                                              cGCCAAAACGGCATTTTATGCCGATGCTCCGAAGAATACGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGt          <  2:667696/138‑1 (MQ=255)                                                                                                                    aaCGGCATTTTATGCCGATGCTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTa  <  2:52332/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                        GCGATAGTAGCGATGTCGATAACGACGGCGACGGAACGTTTTACGCGACAGTGCAGGATTGTATTGATGACGGTAATAATGCCGACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTA  >  NC_000913/4412556‑4412810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑