Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	433,595	T→C	G47G (GGT→GGC)	ribD →	fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	433,595	0	T	C	100.0%	174.4 / NA	52	G47G (GGT→GGC)	ribD	fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

GACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGC  >  NC_000913/433461‑433726                                                                                                                                       |                                                                                                                                    gACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAAcc                                                                                                                                <  1:322194/140‑1 (MQ=255)            cATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAg                                                                                                                     >  2:895908/1‑140 (MQ=255)              tGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTa                                                                                                                   >  1:721995/1‑140 (MQ=255)               ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTac                                                                                                                  >  1:239447/1‑140 (MQ=255)               ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTac                                                                                                                  <  1:895908/140‑1 (MQ=255)               ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTac                                                                                                                  >  1:529593/1‑140 (MQ=255)               ggCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTac                                                                                                                  <  1:520295/140‑1 (MQ=255)                gcgcGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTaca                                                                                                                 >  1:586479/1‑140 (MQ=255)                 cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTacac                                                                                                                >  2:327471/1‑140 (MQ=255)                 cgcgGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTacac                                                                                                                >  2:90241/1‑140 (MQ=255)                    gggCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACAcgcg                                                                                                             >  1:636569/1‑140 (MQ=255)                     ggCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGt                                                                                                            <  2:916967/140‑1 (MQ=255)                       cgcTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTg                                                                                                          <  2:586479/140‑1 (MQ=255)                         cTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCg                                                                                                        <  1:327471/140‑1 (MQ=255)                                  gcgcAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCggg                                                                                               >  2:559267/1‑140 (MQ=255)                                      aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGaa                                                                                           <  1:472390/140‑1 (MQ=255)                                      aaCGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGaa                                                                                           <  2:803372/140‑1 (MQ=255)                                        cGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGaaaa                                                                                         >  2:212326/1‑140 (MQ=255)                                            gACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGcc                                                                                     >  2:301091/1‑140 (MQ=255)                                             aCGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCa                                                                                    <  1:894854/140‑1 (MQ=255)                                                     ccACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCg                                                                            <  1:16910/140‑1 (MQ=255)                                                        cGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGAcc                                                                         >  1:799982/1‑140 (MQ=255)                                                         gCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAAGGGCGAccc                                                                        >  2:279284/1‑139 (MQ=255)                                                          cATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTAAAAAAGCAAAAGGTGCGACCGc                                                                       <  2:636569/140‑1 (MQ=255)                                                          cATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACAAGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAAGCAAAAGGGCGGCCGc                                                                       >  2:804350/1‑140 (MQ=255)                                                           aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGcc                                                                      <  2:239447/140‑1 (MQ=255)                                                           aTCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAGGTGCGACCGcc                                                                      >  1:349216/1‑140 (MQ=255)                                                            ccccACCCCGAAGGTCGGGTGCGTCATTGTCAAGGATGGCGAAATTGTCGGTGAAGGTTCCCCCCACCGTGCGGGCGAACCACATGCCGAAGTCCACGCGTTGCGTAGGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCt                                                                     <  2:871860/139‑1 (MQ=255)                                                               cAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAAGGGCGACCGCCTATg                                                                  >  1:213185/1‑140 (MQ=255)                                                                  cccGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTca                                                               <  1:856786/140‑1 (MQ=255)                                                                       aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTc                                                          <  1:879173/140‑1 (MQ=255)                                                                       aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTTTGTCACACTc                                                          >  2:477656/1‑140 (MQ=255)                                                                       aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGGGCGACCGCCTATGTCACACTc                                                          >  1:387784/1‑140 (MQ=255)                                                                       aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTc                                                          >  1:423355/1‑140 (MQ=255)                                                                       aTGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAACCAAAAGGGCGGCCGCCCATGTCACACTc                                                          >  2:879173/1‑140 (MQ=255)                                                                                gCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGt                                                 <  1:212326/140‑1 (MQ=255)                                                                                 cGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAAACCTGTa                                                >  1:244142/1‑140 (MQ=255)                                                                                  gTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAg                                               >  1:772422/1‑140 (MQ=255)                                                                                         tCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatcat                                        <  2:799982/140‑1 (MQ=255)                                                                                           aaaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAGGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATgg                                      <  1:856658/140‑1 (MQ=255)                                                                                           aaaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTa                                                <  1:702912/130‑1 (MQ=255)                                                                                           aaaGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTa                                                >  2:702912/1‑130 (MQ=255)                                                                                                tGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTa                                 <  1:279284/140‑1 (MQ=255)                                                                                                 ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTAc                                >  2:788166/1‑140 (MQ=255)                                                                                                 ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAACCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTAc                                >  1:139974/1‑140 (MQ=255)                                                                                                 ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAGGTGCGACCGCCCATTTCACACTCGAACCCTGTAGCCCTCATGGTCGTAc                                >  2:856658/1‑140 (MQ=255)                                                                                                 ggCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCCAAAAGTGCGACCGCCCATGTCACACTCCAACCCTGTAGCCATCATGGTCGTAc                                >  2:739784/1‑140 (MQ=255)                                                                                                     aaaTTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                            <  1:301091/140‑1 (MQ=255)                                                                                                     aaaTTGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCa                            <  1:559267/140‑1 (MQ=255)                                                                                                         tGTCGGTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGt                        <  2:349216/140‑1 (MQ=255)                                                                                                              gTGAAGGTTACCACCAACGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgt                   <  1:54496/140‑1 (MQ=255)                                                                                                                              aCGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGGCCGCCTATGTCACACTCGAACCCTGTAGACATCATGTGCGTACGCCACCCAGCTGTGGCGCCCTCCTccccc   >  2:147301/1‑139 (MQ=255)                                                                                                                               cGTGCGGGCGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATcgccgc  >  1:702700/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    GACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGC  >  NC_000913/433461‑433726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑