Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,864	A→G	intergenic (+23/‑7)	decR → / → mdlA	DNA‑binding transcriptional activator DecR/ABC transporter family protein MdlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,864	0	A	G	100.0%	202.9 / NA	56	intergenic (+23/‑7)	decR/mdlA	DNA‑binding transcriptional activator DecR/ABC transporter family protein MdlA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/26);  total (30/26)

TACGACGAGTTTTATAAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCG  >  NC_000913/468731‑469000                                                                                                                                      |                                                                                                                                         tACGACGAGTTTTATAAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAAcgc                                                                                                                                    <  2:135426/140‑1 (MQ=255)  acgacgAGTTTTATAAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAAcgcg                                                                                                                                   >  2:348079/1‑140 (MQ=255)              ataAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTg                                                                                                                       <  1:348079/140‑1 (MQ=255)               taAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTg                                                                                                                                 >  2:117170/1‑129 (MQ=255)               taAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTg                                                                                                                                 <  1:117170/129‑1 (MQ=255)               taAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGc                                                                                                                      >  1:439504/1‑140 (MQ=255)                aaGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCt                                                                                                                     <  1:508391/140‑1 (MQ=255)                    gTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAAt                                                                                                                 >  2:455624/1‑140 (MQ=255)                    gTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACCTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAAt                                                                                                                 >  1:263820/1‑140 (MQ=255)                     tCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAAtt                                                                                                                >  1:523369/1‑140 (MQ=255)                       tGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTaa                                                                                                              >  1:720017/1‑140 (MQ=255)                        ggTAAACAGCGTGCCGGGGCTGTCGGACGTCACCTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAg                                                                                                             <  2:263820/140‑1 (MQ=255)                                gCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTAtt                                                                                                     >  1:715662/1‑140 (MQ=255)                                 cGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTAttt                                                                                                    <  2:261527/140‑1 (MQ=255)                                   tGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTcc                                                                                                  >  1:290529/1‑140 (MQ=255)                                   tGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTcc                                                                                                  >  2:545743/1‑140 (MQ=255)                                   tGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTcc                                                                                                  >  1:464586/1‑140 (MQ=255)                                             gTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATg                                                                                        <  1:568162/140‑1 (MQ=255)                                              tCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATgg                                                                                       >  1:311930/1‑140 (MQ=255)                                                ggACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCg                                                                                     <  1:455624/140‑1 (MQ=255)                                                  aCGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTc                                                                                   >  2:11534/1‑140 (MQ=255)                                                   cGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCg                                                                                  <  2:439504/140‑1 (MQ=255)                                                   cGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCg                                                                                  <  1:524997/140‑1 (MQ=255)                                                    gTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGc                                                                                 <  1:84617/140‑1 (MQ=255)                                                       aCTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTAt                                                                              <  1:480447/140‑1 (MQ=255)                                                            cAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAACATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCgg                                                                         <  2:803609/140‑1 (MQ=255)                                                              gCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCgggg                                                                       >  1:464038/1‑140 (MQ=255)                                                                ttCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCt                                                                     >  2:597492/1‑140 (MQ=255)                                                                      aTGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGTTAATGGCGTCGATATCTCGGGGCTGTCGcc                                                               <  2:682827/140‑1 (MQ=255)                                                                      aTGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGcc                                                               <  1:545743/140‑1 (MQ=255)                                                                         gAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCcttg                                                            <  1:107202/140‑1 (MQ=255)                                                                                      acacCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATcg                                               <  2:464038/140‑1 (MQ=255)                                                                                       caccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATcgc                                              <  1:372749/140‑1 (MQ=255)                                                                                         ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGa                                            >  2:108629/1‑140 (MQ=255)                                                                                         ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGa                                            >  1:871960/1‑140 (MQ=255)                                                                                         ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGa                                            >  1:420551/1‑140 (MQ=255)                                                                                         ccacTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGa                                            >  1:21811/1‑140 (MQ=255)                                                                                             ttcttTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGAtgct                                        >  1:737926/1‑140 (MQ=255)                                                                                               cttTACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGAtgctgc                                      >  1:821699/1‑140 (MQ=255)                                                                                                  tACCCATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAAc                                   >  1:808803/1‑140 (MQ=255)                                                                                                      cATCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGt                               <  1:108629/140‑1 (MQ=255)                                                                                                       aTCGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGtt                              >  1:247138/1‑140 (MQ=255)                                                                                                         cGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTcc                            >  2:886659/1‑140 (MQ=255)                                                                                                         cGAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTAGTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTcc                            >  2:810492/1‑140 (MQ=255)                                                                                                          gAATAAATATCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCg                           >  1:127364/1‑140 (MQ=255)                                                                                                            ataaataTCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGcc                         >  2:574902/1‑140 (MQ=255)                                                                                                                  atCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGt                   <  2:311930/140‑1 (MQ=255)                                                                                                                  atCCAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGt                   <  2:720017/140‑1 (MQ=255)                                                                                                                     cAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggt                <  2:523369/140‑1 (MQ=255)                                                                                                                     cAGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggt                <  2:715662/140‑1 (MQ=255)                                                                                                                      aGAATCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggtt               >  1:477644/1‑140 (MQ=255)                                                                                                                        aaTCAGGTCAGGACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGtggttgg             >  2:593543/1‑140 (MQ=255)                                                                                                                          tcaggtcaggACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTa           >  2:419018/1‑140 (MQ=255)                                                                                                                          tcaggtcaggACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTa           <  2:127364/140‑1 (MQ=255)                                                                                                                                  ggACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTc   <  2:464586/140‑1 (MQ=255)                                                                                                                                   gACGCAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCg  <  2:678990/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TACGACGAGTTTTATAAGCGTCTGGTAAACAGCGTGCCGGGGCTGTCGGACGTCACTTCCAGCTTCGCGATGGAACAGATTAAATACACCACTTCTTTACCCATCGAATAAATATCCAGAATCAGGTCAGGACACAACGCGTGCGATTATTTGCTCAATTAAGCTGGTATTTCCGTCGGGAATGGCGTCGCTATCTCGGGGCTGTCGCCTTGCTTGTCATTATCGCGATGCTGCAACTGGTTCCGCCAAAAGTGGTTGGTATTGTTGTCG  >  NC_000913/468731‑469000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑