Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	543,355	C→T	G231D (GGC→GAC)	allE ←	(S)‑ureidoglycine aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	543,355	0	C	T	100.0%	205.5 / NA	58	G231D (GGC→GAC)	allE	(S)‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

AAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGC  >  NC_000913/543219‑543488                                                                                                                                         |                                                                                                                                      aaaaTCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCa                                                                                                                                    <  1:606254/140‑1 (MQ=255)            tAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTaa                                                                                                                         >  2:102293/1‑140 (MQ=255)             aGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAAt                                                                                                                        <  1:184009/140‑1 (MQ=255)             aGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAAt                                                                                                                        <  1:62854/140‑1 (MQ=255)                            ggCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCg                                                                                                         <  1:871913/140‑1 (MQ=255)                            ggCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCg                                                                                                         <  1:566775/140‑1 (MQ=255)                            ggCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCg                                                                                                         <  1:463255/140‑1 (MQ=255)                            ggCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCg                                                                                                         <  2:399314/140‑1 (MQ=255)                                 gTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATc                                                                                                    >  1:654359/1‑140 (MQ=255)                                  tGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATcc                                                                                                   >  2:114108/1‑140 (MQ=255)                                     tATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGt                                                                                                >  1:589412/1‑140 (MQ=255)                                       tGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTa                                                                                              >  1:758808/1‑140 (MQ=255)                                       tGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTa                                                                                              <  2:800345/140‑1 (MQ=255)                                       tGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCAACACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATATAATCGCCTTTTTTCACCGGGATCCAGTTa                                                                                              >  2:638804/1‑140 (MQ=255)                                           ttAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGt                                                                                          >  2:880720/1‑140 (MQ=255)                                             aaaTCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCg                                                                                        >  1:649388/1‑140 (MQ=255)                                             aaaTCTCTACATCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCg                                                                                        <  1:632795/140‑1 (MQ=255)                                              aaTCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCTCCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGa                                                                                       >  2:372286/1‑140 (MQ=255)                                              aaTCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGa                                                                                       >  2:560387/1‑140 (MQ=255)                                               aTCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAg                                                                                      >  1:49411/1‑140 (MQ=255)                                                 ctctACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGt                                                                                    <  1:114108/140‑1 (MQ=255)                                                     aCGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTtata                                                                                <  2:589412/140‑1 (MQ=255)                                                               tACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGa                                                                      <  1:560387/140‑1 (MQ=255)                                                                aCAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGAc                                                                     <  1:102293/140‑1 (MQ=255)                                                                       ttCGAGTAAATATAGCTGAACGCTTCTCCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAg                                                              <  1:372286/140‑1 (MQ=255)                                                                                   taGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCAcc                                                  <  2:647164/140‑1 (MQ=255)                                                                                   taGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTCACCGGAAAGAATATAGGCAcc                                                  <  2:654359/140‑1 (MQ=255)                                                                                         aaCGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTc                                            >  1:260091/1‑140 (MQ=255)                                                                                         aaCGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTc                                            >  2:361887/1‑140 (MQ=255)                                                                                         aaCGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTc                                            >  1:38820/1‑140 (MQ=255)                                                                                              ttCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGaa                                       >  2:202040/1‑140 (MQ=255)                                                                                              ttCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGaa                                       >  1:818602/1‑140 (MQ=255)                                                                                              ttCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGaa                                       >  1:41242/1‑140 (MQ=255)                                                                                                 accacGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACgt                                    <  2:649388/140‑1 (MQ=255)                                                                                                 accacGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACgt                                    <  1:677289/140‑1 (MQ=255)                                                                                                        ccTACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTc                             >  2:441716/1‑140 (MQ=255)                                                                                                          tACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGa                           >  1:451775/1‑140 (MQ=255)                                                                                                          tACACCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGa                           <  1:283654/140‑1 (MQ=255)                                                                                                            cacCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGata                         <  2:260091/140‑1 (MQ=255)                                                                                                            cacCATAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGata                         <  2:38820/140‑1 (MQ=255)                                                                                                                 tAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAcc                    >  1:777114/1‑140 (MQ=255)                                                                                                                 tAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAcc                    <  2:777114/140‑1 (MQ=255)                                                                                                                 tAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAcc                    <  2:41242/140‑1 (MQ=255)                                                                                                                 tAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAcc                    >  2:84298/1‑140 (MQ=255)                                                                                                                 tAACCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAcc                    >  1:183525/1‑140 (MQ=255)                                                                                                                   aCCAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGt                  <  2:758808/140‑1 (MQ=255)                                                                                                                    ccAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtg                 >  2:37795/1‑140 (MQ=255)                                                                                                                    ccAGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtg                 >  1:449314/1‑140 (MQ=255)                                                                                                                      aGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtggc               >  1:853443/1‑140 (MQ=255)                                                                                                                      aGCCTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtggc               >  1:325437/1‑140 (MQ=255)                                                                                                                         cTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtggctgg            >  2:165630/1‑140 (MQ=255)                                                                                                                         cTGTAAAGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtggctgg            >  1:623399/1‑140 (MQ=255)                                                                                                                             aaaGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCAcc        <  1:687448/140‑1 (MQ=255)                                                                                                                             aaaGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCAcc        <  2:818602/140‑1 (MQ=255)                                                                                                                             aaaGAATAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCAcc        <  1:16920/140‑1 (MQ=255)                                                                                                                                   tAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGt                  >  1:906764/1‑124 (MQ=255)                                                                                                                                   tAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGt                  <  2:906764/124‑1 (MQ=255)                                                                                                                                   tAAGCGTCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGc  <  2:853443/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGC  >  NC_000913/543219‑543488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑