Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	685,011	A→G	V82A (GTA→GCA)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	685,011	0	A	G	100.0%	132.1 / NA	36	V82A (GTA→GCA)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/19);  total (17/19)

AGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGA  >  NC_000913/684879‑685135                                                                                                                                     |                                                                                                                             aGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATccc                                                                                                                       <  2:86737/140‑1 (MQ=255)     cGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGAcg                                                                                                                   >  2:233597/1‑140 (MQ=255)         acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCggg                                                                                                               >  2:368557/1‑140 (MQ=255)         acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCggg                                                                                                               >  1:892071/1‑140 (MQ=255)         acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGACAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGAcgc                                                                                                                  <  1:213709/137‑1 (MQ=255)         acGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGACAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGAcgc                                                                                                                  >  2:213709/1‑137 (MQ=255)            cTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAAc                                                                                                            <  1:233597/140‑1 (MQ=255)            cTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAAc                                                                                                            <  2:187448/140‑1 (MQ=255)                   agTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCtt                                                                                                     <  1:368557/140‑1 (MQ=255)                       tCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTAGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGcc                                                                                                 >  2:197180/1‑140 (MQ=255)                           ggCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGAt                                                                                             <  1:452135/140‑1 (MQ=255)                                tttCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGtt                                                                                        >  1:770169/1‑140 (MQ=255)                                   cACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTc                                                                                     <  2:112523/140‑1 (MQ=255)                                           ggCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCTGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGtt                                                                             <  1:590023/140‑1 (MQ=255)                                           ggCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGtt                                                                             <  2:518449/140‑1 (MQ=255)                                                tttATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCa                                                                        <  1:503309/140‑1 (MQ=255)                                                  tATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACg                                                                      <  1:859704/140‑1 (MQ=255)                                                  tATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACg                                                                      <  2:342781/140‑1 (MQ=255)                                                  tATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACg                                                                      >  2:258533/1‑140 (MQ=255)                                                      gcgTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATAc                                                                  <  2:303914/140‑1 (MQ=255)                                                             aGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGAc                                                           <  2:216025/140‑1 (MQ=255)                                                              gCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACg                                                          <  2:770169/140‑1 (MQ=255)                                                                           cgcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCtt                                             >  2:684252/1‑140 (MQ=255)                                                                            gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                            <  1:684252/140‑1 (MQ=255)                                                                            gcCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTtg                                            >  1:813471/1‑140 (MQ=255)                                                                                  gTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCAc                                      <  1:506912/140‑1 (MQ=255)                                                                                            cGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAgg                            >  2:495117/1‑140 (MQ=255)                                                                                                   cGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                     <  2:813471/140‑1 (MQ=255)                                                                                                   cGACAGATGAGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGa                     <  2:841537/140‑1 (MQ=255)                                                                                                            aGGGAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTaa            >  2:100709/1‑140 (MQ=255)                                                                                                             gggAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCGTTGCCAGATCGGTTTTCTTGTCGTTAGCCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAAt           <  2:97746/140‑1 (MQ=255)                                                                                                              ggAACAGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATc          >  2:751922/1‑140 (MQ=255)                                                                                                                   aGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACTGTTTTAATCAGCGt     >  1:555203/1‑140 (MQ=255)                                                                                                                   aGCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGt     >  1:827942/1‑140 (MQ=255)                                                                                                                    gCTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGtt    >  1:335783/1‑140 (MQ=255)                                                                                                                     cTCGAAATGCTGGAATGCCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTg   >  1:91903/1‑140 (MQ=255)                                                                                                                      tCGAAATGCTGGAATGCCATCACGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGa  >  2:654446/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                             AGCCCGACACGCTCCAGCAGTTTCAGGGCTTTTTCACGAGCCGGCGCTTTATCGCGTTTAAGCACTTTCACCTGCGCCAGGGTCAGGTTTTCGATAATCGACAGATGAGGGAACAGCTCGAAATGCTGGAATACCATCCCGACGCGGGAACGCAGCTTTGCCAGATCGGTTTTCTTGTCGTTAACCACGATACCATCGACGGTGATTTCACCTTGCTGCACCGGTTCGAGGCCGTTGACGGTTTTAATCAGCGTTGA  >  NC_000913/684879‑685135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑