Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	695,193	A→G	V31V (GTA→GTG)	ubiF →	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	695,193	0	A	G	100.0%	198.1 / NA	54	V31V (GTA→GTG)	ubiF	2‑octaprenyl‑3‑methyl‑6‑methoxy‑1,4‑benzoquinol hydroxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/31);  total (23/31)

CCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTAACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTATGCGT  >  NC_000913/695061‑695328                                                                                                                                     |                                                                                                                                         ccTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGcggtgacggtga                                                                                                                                   <  2:379272/140‑1 (MQ=255)                          aCAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGtt                                                                                                         >  1:227843/1‑140 (MQ=255)                          aCAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGtt                                                                                                         >  2:416228/1‑140 (MQ=255)                              gatgatTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTc                                                                                                     >  1:248871/1‑140 (MQ=255)                              gatgatTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTc                                                                                                     >  1:292149/1‑140 (MQ=255)                              gatgatTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTc                                                                                                     >  2:387587/1‑140 (MQ=255)                               atgatTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCg                                                                                                    >  2:622822/1‑140 (MQ=255)                                 gatTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCt                                                                                                  >  1:191037/1‑140 (MQ=255)                                 gatTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCt                                                                                                  >  1:397465/1‑140 (MQ=255)                                      aCCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAg                                                                                             <  2:326016/140‑1 (MQ=255)                                      aCCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAg                                                                                             >  1:572055/1‑140 (MQ=255)                                         aTGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCATGCAGAACCAGCGCCGTTTGTCGCTGATAGCCa                                                                                          >  2:813963/1‑140 (MQ=255)                                          tGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCaa                                                                                         >  2:551975/1‑140 (MQ=255)                                           gACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCTCGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAAc                                                                                        <  1:347434/140‑1 (MQ=255)                                           gACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGC‑GGCCCAGGAAGGAATTGGCGGGGCGGTGATTGGGGCACGCAGAACAACGGCCGTTGGCCG‑TGAAAGCCCAcc                                                                                       >  2:714131/1‑140 (MQ=255)                                             cAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCg                                                                                      >  1:489740/1‑140 (MQ=255)                                              aaaTCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCgg                                                                                     <  2:629995/140‑1 (MQ=255)                                                aTCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGAc                                                                                   >  1:46017/1‑140 (MQ=255)                                                  caaccaacGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGt                                                                                 <  1:38551/140‑1 (MQ=255)                                                      caacGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCgg                                                                             >  2:724915/1‑140 (MQ=255)                                                                 gCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGAt                                                                  <  2:261766/140‑1 (MQ=255)                                                                     ttGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAgc                                                              >  2:344596/1‑140 (MQ=255)                                                                      tGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAgcg                                                             <  1:544107/140‑1 (MQ=255)                                                                       gTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAgcgc                                                            <  2:659837/140‑1 (MQ=255)                                                                          ggcggAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGc                                                         <  1:381565/140‑1 (MQ=255)                                                                               aggaATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCgg                                                    <  1:274638/140‑1 (MQ=255)                                                                                  aaTGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTAt                                                 <  1:724915/140‑1 (MQ=255)                                                                                    tGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCa                                               <  2:131102/140‑1 (MQ=255)                                                                                    tGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCa                                               <  2:849894/140‑1 (MQ=255)                                                                                    tGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCa                                               <  2:248871/140‑1 (MQ=255)                                                                                     ggTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCAt                                              <  1:416228/140‑1 (MQ=255)                                                                                         ggcggcGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCt                                          >  2:738780/1‑140 (MQ=255)                                                                                           ggggccACTGGCGCT‑GGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTaa                                       <  1:714131/135‑1 (MQ=255)                                                                                           cggcgCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTa                                        >  2:185324/1‑140 (MQ=255)                                                                                            ggcgCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTaa                                       <  2:572055/140‑1 (MQ=255)                                                                                            ggcgCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTaa                                       <  2:227843/140‑1 (MQ=255)                                                                                              cgcACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAg                                     >  2:439891/1‑140 (MQ=255)                                                                                                    ggCGCTGGGGCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAg                               <  1:551975/140‑1 (MQ=255)                                                                                                          ggggCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCt                         >  2:386168/1‑140 (MQ=255)                                                                                                           gggCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTg                        <  1:622822/140‑1 (MQ=255)                                                                                                           gggCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTg                        <  2:46017/140‑1 (MQ=255)                                                                                                             gCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTggg                      <  1:678146/140‑1 (MQ=255)                                                                                                             gCTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTggg                      <  2:397465/140‑1 (MQ=255)                                                                                                              cTGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGa                     <  2:292149/140‑1 (MQ=255)                                                                                                               tGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGAt                    <  2:489740/140‑1 (MQ=255)                                                                                                               tGGCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGAt                    >  2:649742/1‑140 (MQ=255)                                                                                                                ggCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATg                   <  2:305416/140‑1 (MQ=255)                                                                                                                ggCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATg                   <  2:191037/140‑1 (MQ=255)                                                                                                                ggCACAGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATg                   <  1:20680/140‑1 (MQ=255)                                                                                                                     aGCACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTa              <  1:205840/140‑1 (MQ=255)                                                                                                                       cACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACa            >  2:51323/1‑140 (MQ=255)                                                                                                                       cACGGATTTGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACa            >  2:274370/1‑140 (MQ=255)                                                                                                                            aTTTGCGGTGACGGTGA‑TCGAGC‑CGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTAt      <  1:525789/140‑1 (MQ=255)                                                                                                                             tttGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTAt      <  1:439891/140‑1 (MQ=255)                                                                                                                              ttGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTATg     <  1:51323/140‑1 (MQ=255)                                                                                                                               tGCGGTGACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTATGc    >  2:220782/1‑140 (MQ=255)                                                                                                                                 cggtgacggtga‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTATGCGt  >  2:12053/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         CCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGATTTGCGGTAACGGTGA‑TCGAGCACGCAGAACCAGCGCCGTTTGTCGCTGATAGCCAACCGGACGTGCGGATCTCGGCGATCAGCGCGGCTTCGGTATCATTGCTTAAAGGGTTAGGGGTCTGGGATGCAGTACAGGCTATGCGT  >  NC_000913/695061‑695328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑