Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	124,351	T→C	G445G (GGT→GGC)	aceE →	pyruvate dehydrogenase E1 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	124,351	0	T	C	100.0%	258.4 / NA	70	G445G (GGT→GGC)	aceE	pyruvate dehydrogenase E1 component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/32);  total (38/32)

GGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCA  >  NC_000913/124216‑124487                                                                                                                                        |                                                                                                                                         ggCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTg                                                                                                                                      <  2:414914/140‑1 (MQ=255) ggCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTg                                                                                                                                      <  1:645225/140‑1 (MQ=255)   cTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGaa                                                                                                                                    <  1:665324/140‑1 (MQ=255)     gAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAaga                                                                                                                                  <  1:766411/140‑1 (MQ=255)     gAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAaga                                                                                                                                  <  2:500368/140‑1 (MQ=255)        ggTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCa                                                                                                                               <  2:182086/140‑1 (MQ=255)             aaaCATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGCCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCt                                                                                                                          >  1:631059/1‑140 (MQ=255)                   cgcgCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGc                                                                                                                    >  2:267346/1‑140 (MQ=255)                   cgcgCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGc                                                                                                                    >  1:697584/1‑140 (MQ=255)                   cgcgCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGc                                                                                                                    >  2:464540/1‑140 (MQ=255)                                         aaCATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCa                                                                                              <  2:213333/140‑1 (MQ=255)                                         aaCATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCa                                                                                              <  1:704910/140‑1 (MQ=255)                                           cATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACg                                                                                            <  2:577696/140‑1 (MQ=255)                                            aTGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACgg                                                                                           <  1:45457/140‑1 (MQ=255)                                             tGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGt                                                                                          >  2:445464/1‑140 (MQ=255)                                              ggACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGtt                                                                                         <  2:173735/140‑1 (MQ=255)                                              ggACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGtt                                                                                         >  1:299200/1‑140 (MQ=255)                                              ggACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGtt                                                                                         >  2:907527/1‑140 (MQ=255)                                                aCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTAt                                                                                       >  2:402359/1‑140 (MQ=255)                                                aCGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTAt                                                                                       >  1:884155/1‑140 (MQ=255)                                                   gtgtGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTg                                                                                    >  2:791251/1‑140 (MQ=255)                                                    tgtgCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGc                                                                                   >  2:575687/1‑140 (MQ=255)                                                        cGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAg                                                                               >  1:611756/1‑140 (MQ=255)                                                         gTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGc                                                                              >  1:618133/1‑140 (MQ=255)                                                              atCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCa                                                                         >  1:568613/1‑140 (MQ=255)                                                              atCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCa                                                                         >  2:492668/1‑140 (MQ=255)                                                              atCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCa                                                                         >  1:393904/1‑140 (MQ=255)                                                              atCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCa                                                                         >  1:304156/1‑140 (MQ=255)                                                              atCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCa                                                                         >  1:854290/1‑140 (MQ=255)                                                              atCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCa                                                                         >  2:327004/1‑140 (MQ=255)                                                               tCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAg                                                                        <  1:314735/140‑1 (MQ=255)                                                                    gACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGaa                                                                   >  1:244575/1‑140 (MQ=255)                                                                    gACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGaa                                                                   <  1:907527/140‑1 (MQ=255)                                                                    gACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGaa                                                                   <  1:464540/140‑1 (MQ=255)                                                                        gTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTc                                                               >  2:97376/1‑140 (MQ=255)                                                                         tttCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCa                                                              >  1:900855/1‑140 (MQ=255)                                                                          ttCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCAc                                                             <  2:900855/140‑1 (MQ=255)                                                                          ttCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGGACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCACCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCAc                                                             >  1:896136/1‑140 (MQ=255)                                                                                  gCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGc                                                     <  2:105760/140‑1 (MQ=255)                                                                                  gCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGc                                                     <  2:244575/140‑1 (MQ=255)                                                                                   ccGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCt                                                    >  1:890914/1‑140 (MQ=255)                                                                                   ccGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCt                                                    >  2:70687/1‑140 (MQ=255)                                                                                       tgtCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCGCGGTTATCTGCCAAGCCGTCAGCCGAACTTCCCCGAGAAGCTTGAg                                                <  2:584879/140‑1 (MQ=255)                                                                                        gtCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGc                                               >  1:829724/1‑140 (MQ=255)                                                                                           tGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGc                                            >  1:472566/1‑140 (MQ=255)                                                                                           tGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGc                                            <  1:788267/140‑1 (MQ=255)                                                                                             aTGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCg                                          >  1:137356/1‑140 (MQ=255)                                                                                             aTGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCg                                          >  2:835840/1‑140 (MQ=255)                                                                                              tGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGa                                         <  2:299200/140‑1 (MQ=255)                                                                                                   atatCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTg                                    >  1:427524/1‑140 (MQ=255)                                                                                                   atatCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTg                                    >  2:905639/1‑140 (MQ=255)                                                                                                     atCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCa                                  <  2:854290/140‑1 (MQ=255)                                                                                                     atCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCa                                  >  2:433983/1‑140 (MQ=255)                                                                                                     atCGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCa                                  >  1:862684/1‑140 (MQ=255)                                                                                                       cGAAAAACTGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAg                                >  2:82780/1‑140 (MQ=255)                                                                                                               tGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgc                        <  2:890914/140‑1 (MQ=255)                                                                                                               tGCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgc                        >  2:491487/1‑140 (MQ=255)                                                                                                                gCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcg                       <  1:905639/140‑1 (MQ=255)                                                                                                                gCCGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcg                       <  2:401391/140‑1 (MQ=255)                                                                                                                gCCGGACATCACCTTCCCGGAAGGCTCTGAAGAGCATCCCTATCGTCACGCCCAGCGTCAGAAACTGCAGGGTTTTCTTCCAAGCCGTCAGCCGAGCTCCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcg                       <  2:401390/140‑1 (MQ=255)                                                                                                                 ccGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgc                      <  1:267146/140‑1 (MQ=255)                                                                                                                 ccGTACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcgc                      <  2:884155/140‑1 (MQ=255)                                                                                                                    tACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCt                   <  2:472566/140‑1 (MQ=255)                                                                                                                    tACATCACCTTCCCGGAAGGCTCTGAAGAGCATACCTATCCGCACGCTCAGGGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCt                   <  1:654430/140‑1 (MQ=255)                                                                                                                           ccTTCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGaa            >  1:5407/1‑140 (MQ=255)                                                                                                                              tCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAGCTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcg                       <  2:61668/126‑1 (MQ=255)                                                                                                                              tCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGgcg                       >  1:61668/1‑126 (MQ=255)                                                                                                                              tCCCGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagag         <  2:281201/140‑1 (MQ=255)                                                                                                                                ccGGAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAGGACTTCGGCGCGCTGTTGGAagagca       >  2:260548/1‑140 (MQ=255)                                                                                                                                  ggAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagagcaga     <  2:786297/140‑1 (MQ=255)                                                                                                                                  ggAAGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAagagcaga     <  2:427524/140‑1 (MQ=255)                                                                                                                                     aGGCTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAgagcagagca  <  2:449997/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GGCTGAAGGTAAAAACATCGCGCACCAGGTTAAGAAAATGAACATGGACGGTGTGCGTCATATCCGCGACCGTTTCAATGTGCCGGTGTCTGATGCAGATATCGAAAAACTGCCGTACATCACCTTCCCGGAAGGTTCTGAAGAGCATACCTATCTGCACGCTCAGCGTCAGAAACTGCACGGTTATCTGCCAAGCCGTCAGCCGAACTTCACCGAGAAGCTTGAGCTGCCGAGCCTGCAAGACTTCGGCGCGCTGTTGGAAGAGCAGAGCA  >  NC_000913/124216‑124487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑