Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	751,395	A→G	L259L (TTA→CTA)	ybgQ ←	putative fimbrial usher protein YbgQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	751,395	0	A	G	100.0%	174.7 / NA	50	L259L (TTA→CTA)	ybgQ	putative fimbrial usher protein YbgQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/21);  total (29/21)

GTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGC  >  NC_000913/751261‑751523                                                                                                                                       |                                                                                                                                 gTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCAtgc                                                                                                                             >  2:853331/1‑138 (MQ=255) gTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTc                                                                                                                             >  1:311043/1‑140 (MQ=255) gTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTc                                                                                                                             >  1:372664/1‑140 (MQ=255)    atcaATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGAt                                                                                                                          <  1:847564/140‑1 (MQ=255)      caATAATAAACGGGCCTGGCGGGGCTTTTTTCTGGTAAATGACGCGGGCTGATTGACTGCTCGTCACCGTGGCATTGGGCTGTGCAATACCGCTAATTTGTGGGGGGTAACCACGCAGCTCCCACGGTAGCATTCGATCg                                                                                                                        >  2:162633/1‑140 (MQ=255)           ataaACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGAt                                                                                                                          <  1:218765/133‑1 (MQ=255)           ataaACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGAt                                                                                                                          >  2:218765/1‑133 (MQ=255)                cGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCa                                                                                                              >  2:105876/1‑140 (MQ=255)                 gggCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAg                                                                                                             >  2:475957/1‑140 (MQ=255)                      tGGCGGGACTTTTTTCTGGTAAATGGCGGGGGCTGATTGACTGGTCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAACTCCCACGGTAGCCTTCCATCGTCACTCGCCAGTGCcg                                                                                                        >  2:509019/1‑140 (MQ=255)                         cGGGCCTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                     <  1:105876/140‑1 (MQ=255)                         cGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                     <  1:150871/140‑1 (MQ=255)                         cGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                     <  2:286477/140‑1 (MQ=255)                         cGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                     <  1:842813/140‑1 (MQ=255)                         cGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                     <  1:520711/140‑1 (MQ=255)                         cGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                     <  1:509019/140‑1 (MQ=255)                         cGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCCAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                     <  2:543086/140‑1 (MQ=255)                             aCTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCcgcg                                                                                                      <  1:78497/135‑1 (MQ=255)                             aCTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCcgcg                                                                                                      >  2:78497/1‑135 (MQ=255)                             aCTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGt                                                                                                 >  2:799037/1‑140 (MQ=255)                             aCTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGt                                                                                                 >  2:282059/1‑140 (MQ=255)                             aCTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGt                                                                                                 >  1:446808/1‑140 (MQ=255)                               ttttttCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGtat                                                                                               <  1:50762/140‑1 (MQ=255)                                           aaTGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCAt                                                                                   >  2:122547/1‑140 (MQ=255)                                              gACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGa                                                                                <  1:280188/140‑1 (MQ=255)                                                  cgGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAat                                                                            >  2:147941/1‑140 (MQ=255)                                                  cgGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAat                                                                            >  2:724560/1‑140 (MQ=255)                                                  cgGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAat                                                                            >  2:337285/1‑140 (MQ=255)                                                      cTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTg                                                                        <  1:86063/140‑1 (MQ=255)                                                        gATTGACTGATCGTCACCGTGACATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGa                                                                      >  1:897126/1‑140 (MQ=255)                                                                   cGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCgg                                                           >  2:790733/1‑140 (MQ=255)                                                                           tGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                   >  1:834451/1‑140 (MQ=255)                                                                           tGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                   >  2:388817/1‑140 (MQ=255)                                                                            ggCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                   >  1:778240/1‑139 (MQ=255)                                                                            ggCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                   <  2:778240/139‑1 (MQ=255)                                                                                ttGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGt                                              <  2:446808/140‑1 (MQ=255)                                                                                   gTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTaa                                           >  2:432255/1‑140 (MQ=255)                                                                                       gtgCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTa                                       <  2:336166/140‑1 (MQ=255)                                                                                           aaTACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGc                                   <  2:897126/140‑1 (MQ=255)                                                                                             tACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCt                                 <  1:282059/140‑1 (MQ=255)                                                                                                  cTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTg                            >  1:180397/1‑140 (MQ=255)                                                                                                  cTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTg                            <  1:799037/140‑1 (MQ=255)                                                                                                  cTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTg                            >  1:612130/1‑140 (MQ=255)                                                                                                           ggggCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATgg                   <  2:409881/140‑1 (MQ=255)                                                                                                            gggCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGa                  >  2:582724/1‑140 (MQ=255)                                                                                                            gggCGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGa                  >  2:289847/1‑140 (MQ=255)                                                                                                               cGTAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGa               >  1:2500/1‑140 (MQ=255)                                                                                                                 tAGCCACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGaaa             >  2:749636/1‑140 (MQ=255)                                                                                                                    ccACGCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAg          >  1:176802/1‑140 (MQ=255)                                                                                                                        gCAGCTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAg      <  2:311043/140‑1 (MQ=255)                                                                                                                            cTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTgc  >  2:644835/1‑140 (MQ=255)                                                                                                                            cTCCCACGGTAGCATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTgc  >  2:732836/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 GTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGC  >  NC_000913/751261‑751523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑