Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	752,266	A→G	V177A (GTA→GCA)	ybgD ←	putative fimbrial protein YbgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	752,266	0	A	G	100.0%	187.6 / NA	54	V177A (GTA→GCA)	ybgD	putative fimbrial protein YbgD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/19);  total (35/19)

ACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTT  >  NC_000913/752132‑752400                                                                                                                                       |                                                                                                                                       aCCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCa                                                                                                                                   >  2:513081/1‑140 (MQ=255)   caggcaggATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCAcc                                                                                                                                 <  1:640249/140‑1 (MQ=255)           aTACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGa                                                                                                                         >  2:586668/1‑140 (MQ=255)           aTACAAAAGAGAGTCGATAAATATTCACGGAGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGa                                                                                                                         >  1:413354/1‑140 (MQ=255)                    agagTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTg                                                                                                                >  1:739446/1‑140 (MQ=255)                    agagTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTg                                                                                                                >  2:292240/1‑140 (MQ=255)                           aTAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATc                                                                                                         >  2:596202/1‑140 (MQ=255)                              aaTATTCACGGGGTCAATACCTGATAAATATTTTATGAAAGGCGGCGAAGATGCCGCCAAAATAATCCTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCCCCTGCCGTGACATCGACTGCATTATCAAt                                                                                                      <  2:413354/140‑1 (MQ=255)                                   tCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGtt                                                                                                 <  1:292240/140‑1 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  2:576543/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  1:660404/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  1:777407/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  1:878052/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  2:440635/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  2:47000/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  2:59789/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  1:477000/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  2:723508/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  2:813869/1‑140 (MQ=255)                                                     aTAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGt                                                                               >  2:849732/1‑140 (MQ=255)                                                       aaaTATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTc                                                                             <  2:739446/140‑1 (MQ=255)                                                            ttttATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGt                                                                        <  2:122831/140‑1 (MQ=255)                                                            ttttATGAAAGGCGGCGATGATGCCGCAAAAATAACACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGt                                                                        <  2:244554/140‑1 (MQ=255)                                                                aTGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGt                                                                    <  1:723508/140‑1 (MQ=255)                                                                      ggcggcGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCtgat                                                              <  1:642773/140‑1 (MQ=255)                                                                      ggcggcGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCtgat                                                              <  2:477000/140‑1 (MQ=255)                                                                       gcggcgATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCtgatg                                                             <  1:576543/140‑1 (MQ=255)                                                                       gcggcgATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCtgatg                                                             <  1:596202/140‑1 (MQ=255)                                                                          gcgATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGc                                                          <  1:586668/140‑1 (MQ=255)                                                                           cgATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGTGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCt                                                         >  1:101010/1‑140 (MQ=255)                                                                                 tgCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATc                                                   <  1:513081/140‑1 (MQ=255)                                                                                 tgCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATc                                                   >  1:618422/1‑140 (MQ=255)                                                                                 tgCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATc                                                   >  1:590763/1‑140 (MQ=255)                                                                                    cgccAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAg                                                <  1:391871/140‑1 (MQ=255)                                                                                          aataatACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAg                                          >  1:245116/1‑140 (MQ=255)                                                                                                    atttatAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGcgcg                                >  1:916514/1‑140 (MQ=255)                                                                                                     tttatAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTc                                             <  2:542942/126‑1 (MQ=255)                                                                                                     tttatAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTc                                             >  1:542942/1‑126 (MQ=255)                                                                                                      ttatAATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGc                              >  1:4772/1‑140 (MQ=255)                                                                                                       tataATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCt                             <  1:813869/140‑1 (MQ=255)                                                                                                         taATCCAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGa                           <  2:777407/140‑1 (MQ=255)                                                                                                             ccAGCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCt                       <  1:59789/140‑1 (MQ=255)                                                                                                                gCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTgg                                    >  2:410481/1‑124 (MQ=255)                                                                                                                gCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTgg                                    <  1:410481/124‑1 (MQ=255)                                                                                                                gCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAAt                    >  2:84593/1‑140 (MQ=255)                                                                                                                gCACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAAt                    >  2:835605/1‑140 (MQ=255)                                                                                                                 cACGTAGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATa                   >  1:618915/1‑140 (MQ=255)                                                                                                                      aGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCTAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATg              >  2:837991/1‑140 (MQ=255)                                                                                                                      aGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATg              >  2:751644/1‑140 (MQ=255)                                                                                                                      aGGTTGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATg              >  2:340893/1‑140 (MQ=255)                                                                                                                          tGCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTAc          >  2:582711/1‑140 (MQ=255)                                                                                                                           gCGTTAGCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTAcc         >  1:135730/1‑140 (MQ=255)                                                                                                                                 gCGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCAtt   >  2:197700/1‑140 (MQ=255)                                                                                                                                  cGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCAttt  <  2:590763/140‑1 (MQ=255)                                                                                                                                  cGGTTGCTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCAttt  >  2:832017/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       ACCAGGCAGGATACAAAAGAGAGTCGATAAATATTCACGGTGTCCATACCTGATAAATATTTTATGAAAGGCGGCGATGATGCCGCCAAAATAATACTTATTTATAATCCAGCACGTAGGTTGCGTTAGCGGTTACTTCACCTGCCGTGACATCGACTGCATTATCAATTTGTTCCATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTT  >  NC_000913/752132‑752400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑