BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	766,784	C→T	A267A (GCC→GCT)	mngA →	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	766,784	0	C	T	100.0%	118.4 / NA	34	A267A (GCC→GCT)	mngA	2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/18); total (16/18)

AAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAG  >  NC_000913/766648‑766918

agggCGCAAGCGGCTTTGAGGGGTGTTAAAGGGGGGATAAACTAAATAGTACAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTgctgag                                                                                                                                     <  2:319683/138‑1 (MQ=255)

              aTTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGc                                                                                                                       >  1:631269/1‑140 (MQ=255)

                                      cAACTAAATGGTGAAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCt                                                                                               <  1:316526/140‑1 (MQ=255)

                                         cTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCcaa                                                                                                       <  2:624779/129‑1 (MQ=255)

                                         cTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCcaa                                                                                                       >  1:624779/1‑129 (MQ=255)

                                             aTAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTc                                                                                        >  2:827868/1‑140 (MQ=255)

                                                 tGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGc                                                                                    >  1:350701/1‑140 (MQ=255)

                                                   cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGt                                                                                  >  2:520256/1‑140 (MQ=255)

                                                   cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGt                                                                                  >  1:516849/1‑140 (MQ=255)

                                                   cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGt                                                                                  >  1:607428/1‑140 (MQ=255)

                                                   cAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGt                                                                                  >  2:279420/1‑140 (MQ=255)

                                                      ccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCt                                                                                               >  2:195534/1‑124 (MQ=255)

                                                      ccGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCt                                                                                               <  1:195534/124‑1 (MQ=255)

                                                         ccTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGAt                                                                            >  1:39996/1‑140 (MQ=255)

                                                           tGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATga                                                                          >  1:786/1‑140 (MQ=255)

                                                              aTTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGAc                                                                       <  2:138923/140‑1 (MQ=255)

                                                                   tGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGcgta                                                                  <  1:827868/140‑1 (MQ=255)

                                                                   tGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGcgta                                                                  <  1:279420/140‑1 (MQ=255)

                                                                           aaGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCTTGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAg                                                          <  2:631269/140‑1 (MQ=255)

                                                                             gTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCa                                                        <  2:743916/140‑1 (MQ=255)

                                                                              tCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCaa                                                       <  2:873422/140‑1 (MQ=255)

                                                                                            agTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGt                                         >  1:479413/1‑140 (MQ=255)

                                                                                              tGAGTGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGa                                       >  2:123460/1‑140 (MQ=255)

                                                                                              tGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGa                                       >  1:738042/1‑140 (MQ=255)

                                                                                              tGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGa                                       >  2:720534/1‑140 (MQ=255)

                                                                                                aGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGCTCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGaaa                                     >  1:68603/1‑140 (MQ=255)

                                                                                                aGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGaaa                                     <  2:154815/140‑1 (MQ=255)

                                                                                                aGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGaaa                                     >  2:848333/1‑140 (MQ=255)

                                                                                                          tGGCATTCCCGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCaaaa                           <  2:435604/140‑1 (MQ=255)

                                                                                                                 cccGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCt                    <  1:520256/140‑1 (MQ=255)

                                                                                                                 cccGCGCTTTCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCt                    <  2:881186/140‑1 (MQ=255)

                                                                                                                         ttCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAgg            <  2:607428/140‑1 (MQ=255)

                                                                                                                         ttCAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAgg            <  2:479413/140‑1 (MQ=255)

                                                                                                                           cAGTGCCTGTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCa          <  1:720534/140‑1 (MQ=255)

                                                                                                                                   gTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAg  <  2:738042/140‑1 (MQ=255)

                                                                                                                                   gTTGCTGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAg  <  2:39996/140‑1 (MQ=255)

AAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAATGGCATTCCCGCGCTTTCAGTGCCTGTTGCCGAGCCGATTCGCCATGCAGAAGCGTTGATCCAACAAGCGCTTACCCTCAAGCGTAGCGATGAGACGCGTACCGTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAG  >  NC_000913/766648‑766918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`