Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	784,507	A→G	V106A (GTC→GCC)	zitB ←	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	784,507	0	A	G	100.0%	203.5 / NA	55	V106A (GTC→GCC)	zitB	Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/34);  total (21/34)

TGAGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAG  >  NC_000913/784370‑784640                                                                                                                                          |                                                                                                                                      tGAGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCa                                                                                                                                     >  2:70656/1‑140 (MQ=255)  gAGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCaa                                                                                                                                    >  2:687942/1‑140 (MQ=255)  gAGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAACCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCaa                                                                                                                                    >  2:275786/1‑140 (MQ=255)   aGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAAt                                                                                                                                   >  2:208289/1‑140 (MQ=255)   aGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAAt                                                                                                                                   <  2:203915/140‑1 (MQ=255)   aGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAAt                                                                                                                                   <  1:872279/140‑1 (MQ=255)   aGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAAt                                                                                                                                   <  1:383426/140‑1 (MQ=255)       ttttCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCaaa                                                                                                                               <  1:109776/140‑1 (MQ=255)       ttttCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCaaa                                                                                                                               <  1:803367/140‑1 (MQ=255)         ttCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAAt                                                                                                                             <  1:47577/140‑1 (MQ=255)         ttCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAAt                                                                                                                             <  1:700627/140‑1 (MQ=255)          tctcTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCGGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATg                                                                                                                            <  2:887154/140‑1 (MQ=255)           ctctTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATgg                                                                                                                           <  1:19290/140‑1 (MQ=255)             ctTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTa                                                                                                                         >  2:221342/1‑140 (MQ=255)                      cGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACg                                                                                                                <  2:726062/140‑1 (MQ=255)                       gTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGa                                                                                                               <  1:788245/140‑1 (MQ=255)                            gaAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCt                                                                                                          <  1:687942/140‑1 (MQ=255)                             aaGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTa                                                                                                         <  1:70656/140‑1 (MQ=255)                             aaGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTa                                                                                                         <  2:882759/140‑1 (MQ=255)                               gTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATg                                                                                                       <  1:208289/140‑1 (MQ=255)                                tAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATGGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAGTCAAAATGGTAATTACCACCAGGGCTATgg                                                                                                      >  2:10142/1‑140 (MQ=255)                                     agaaagaaAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGa                                                                                                               <  2:768322/126‑1 (MQ=255)                                     agaaagaaAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGa                                                                                                               >  1:768322/1‑126 (MQ=255)                                       aaagaaagTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTcac                                                                                               >  1:415579/1‑140 (MQ=255)                                         agaaagTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACaa                                                                                             >  2:444730/1‑140 (MQ=255)                                                 tatTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCcgcg                                                                                     >  1:457611/1‑140 (MQ=255)                                                 tatTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCcgcg                                                                                     >  2:747753/1‑140 (MQ=255)                                                  atTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGa                                                                                    <  2:232558/140‑1 (MQ=255)                                                  atTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGa                                                                                    <  2:509583/140‑1 (MQ=255)                                                   tttGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAg                                                                                   <  1:841196/140‑1 (MQ=255)                                                    ttGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGc                                                                                  <  1:754989/140‑1 (MQ=255)                                                    ttGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGAACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGc                                                                                  <  1:557434/140‑1 (MQ=255)                                                      gCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGt                                                                                >  2:559302/1‑140 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGTAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  2:825858/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  1:467994/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  2:394883/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  2:797753/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  2:81029/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  2:293414/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  2:907653/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             <  1:627644/140‑1 (MQ=255)                                                         aGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGt                                                                             >  1:482925/1‑140 (MQ=255)                                                           cAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCa                                                                           >  2:137901/1‑140 (MQ=255)                                                                       ggCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAAc                                                               <  2:415579/140‑1 (MQ=255)                                                                          aaTTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGa                                                            <  1:137901/140‑1 (MQ=255)                                                                                 atcatcatGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGtgtg                                                     >  2:918371/1‑140 (MQ=255)                                                                                   catcatGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGc                                                   <  2:520089/140‑1 (MQ=255)                                                                                   catcatGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGc                                                   >  2:303268/1‑140 (MQ=255)                                                                                       atGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAAt                                               >  2:32790/1‑140 (MQ=255)                                                                                         gccgccCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAAtgg                                             <  1:368917/140‑1 (MQ=255)                                                                                                    ccGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGgacga                                  <  1:380101/140‑1 (MQ=255)                                                                                                              cgTGCGGAAGCGTTCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAAtt                        >  1:370568/1‑140 (MQ=255)                                                                                                                          ttCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTa            >  2:551038/1‑140 (MQ=255)                                                                                                                          ttCTATCGCCTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTa            >  2:230724/1‑140 (MQ=255)                                                                                                                                  ccTCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGaaa    >  2:866335/1‑140 (MQ=255)                                                                                                                                    tCCCAGGCAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAg  >  2:531946/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TGAGGTTTTTCTCTTCGCTGCCGTGATGAAGTAACCAGAAAGAAAGTATATTTGCCAGCAACCCAGCCACGGCAATTGCCATCATCATGCCGCCCTCGACCGGACGCGGCGTGCGGAAGCGTTCTATCGCCTCCCAGACAATCAAAATGGTAATTACCACGAGGGCTATGGCATTCACAAACGCCGCGAGCGTGGTCAGTCTTAGCCAACCGAAAGTGTGGCGAATGGTGGGAGGACGACGTGAAAATTGCACGGCGAGTAGGGCGAAAAG  >  NC_000913/784370‑784640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑