Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	923,371	T→C	S36S (AGT→AGC)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	923,371	0	T	C	100.0%	171.5 / NA	47	S36S (AGT→AGC)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

CAGGCATAAAAATTGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTCTGCCTGCCAGTGAAGAGGAGCGCGACACACAGCCG  >  NC_000913/923237‑923503                                                                                                                                       |                                                                                                                                     cAGGCATAAAAATTGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAAccc                                                                                                                                 <  2:79047/140‑1 (MQ=255)   ggCATAAAAATTGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCAt                                                                                                                               <  1:812526/140‑1 (MQ=255)   ggCATAAAAATTGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCAt                                                                                                                               >  1:414636/1‑140 (MQ=255)    gCATAAAAATTGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATc                                                                                                                              >  1:343831/1‑140 (MQ=255)         aaaaTTGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGccc                                                                                                                         >  1:638141/1‑140 (MQ=255)             ttGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGa                                                                                                                     <  1:54058/140‑1 (MQ=255)                ggggAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGgc                                                                                                                  >  1:535807/1‑140 (MQ=255)                     ggTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTgg                                                                                                             >  2:320899/1‑140 (MQ=255)                      gTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGa                                                                                                            <  2:414636/140‑1 (MQ=255)                            aTGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCgtg                                                                                                      <  2:413967/140‑1 (MQ=255)                                tcaatcaaGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCt                                                                                                  >  1:892406/1‑140 (MQ=255)                                   atcaaGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCtgtg                                                                                               >  2:901018/1‑140 (MQ=255)                                   atcaaGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCt                                                                                                  >  2:187371/1‑137 (MQ=255)                                   atcaaGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCt                                                                                                  <  1:187371/137‑1 (MQ=255)                                       aGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGAtt                                                                                           <  1:375873/140‑1 (MQ=255)                                         aaCTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTg                                                                                         >  1:550855/1‑140 (MQ=255)                                              gAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTgc                                                                                    >  2:160824/1‑140 (MQ=255)                                              gAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTgc                                                                                    >  1:261222/1‑140 (MQ=255)                                               aaCTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGATGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTgcg                                                                                   <  2:638141/140‑1 (MQ=255)                                                    aGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCg                                                                              >  2:542060/1‑140 (MQ=255)                                                                gCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGa                                                                  >  2:284004/1‑140 (MQ=255)                                                                gCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGa                                                                  <  1:22774/140‑1 (MQ=255)                                                                gCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGa                                                                  >  2:753188/1‑140 (MQ=255)                                                                gCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGa                                                                  >  1:269711/1‑140 (MQ=255)                                                                gCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGa                                                                  >  2:854680/1‑140 (MQ=255)                                                                    tCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGcc                                                              >  2:807438/1‑140 (MQ=255)                                                                     cGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCt                                                             >  1:402825/1‑140 (MQ=255)                                                                     cGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCt                                                             <  1:542060/140‑1 (MQ=255)                                                                        cAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTa                                                          <  2:589754/140‑1 (MQ=255)                                                                             cgcgcgAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGaa                                                     <  2:521793/140‑1 (MQ=255)                                                                               cgcgAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACa                                                   >  1:57482/1‑140 (MQ=255)                                                                               cgcgAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACa                                                   <  2:535807/140‑1 (MQ=255)                                                                                      aCCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACcaca                                            <  2:364242/140‑1 (MQ=255)                                                                                          tCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCg                                        <  1:129500/140‑1 (MQ=255)                                                                                          tCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCg                                        >  2:129500/1‑140 (MQ=255)                                                                                          tCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCg                                        >  1:88893/1‑140 (MQ=255)                                                                                                    aTGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTctgcctgc                              <  1:160824/140‑1 (MQ=255)                                                                                                    aTGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTctgcctgc                              <  2:892406/140‑1 (MQ=255)                                                                                                    aTGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTctgcctgc                              <  2:57482/140‑1 (MQ=255)                                                                                                    aTGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTctgcctgc                              <  2:402825/140‑1 (MQ=255)                                                                                                    aTGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTctgcctgc                              <  2:343831/140‑1 (MQ=255)                                                                                                    aTGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTctgcctgc                              <  1:320899/140‑1 (MQ=255)                                                                                                    aTGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTctgcctgc                              <  1:901018/140‑1 (MQ=255)                                                                                                              aGCACTTGTAACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTCCGCCCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTCTGCCTGCCAGTGAagag                    <  2:235908/140‑1 (MQ=255)                                                                                                                   ttGTTACTGGCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTCTTCCTGCCAGTGAAGAGGAgcg               <  2:88893/140‑1 (MQ=255)                                                                                                                           ggCGCTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTCTGCCTGCCAGTGAAGAGGAGCGCGacacac       >  2:126763/1‑140 (MQ=255)                                                                                                                             cgcTGCTCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTCTGCCTGCCAGTGAAGAGGAGCGCGACACACAg     >  1:189493/1‑140 (MQ=255)                                                                                                                                tgctCAGCAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTCTGCCTGCCAGTGAAGAGGAGCGCGACACACAGCCg  >  2:380441/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     CAGGCATAAAAATTGGGGGAGGTGCCTATGCTCAATCAAGAACTGGAACTCAGTTTAAATATGGCTTTCGCCAGAGCGCGCGAGCACCGTCATGAGTTTATGACCGTCGAGCACTTGTTACTGGCGCTGCTCAGTAACCCATCTGCCCGGGAGGCGCTGGAAGCGTGTTCTGTGGATTTGGTTGCGCTCCGTCAGGAACTGGAAGCCTTTATTGAACAAACCACACCCGTTCTGCCTGCCAGTGAAGAGGAGCGCGACACACAGCCG  >  NC_000913/923237‑923503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑