Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,000,388	A→C	G391G (GGA→GGC)	elfC →	putative fimbrial usher protein ElfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,000,388	0	A	C	100.0%	195.8 / NA	53	G391G (GGA→GGC)	elfC	putative fimbrial usher protein ElfC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (25/28);  total (25/28)

TACCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGACCCAGGCGAAGAGTACGCTAGCAG  >  NC_000913/1000255‑1000524                                                                                                                                      |                                                                                                                                         tACCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTgg                                                                                                                                    >  1:202948/1‑140 (MQ=255)  aCCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTggg                                                                                                                                   >  2:586968/1‑140 (MQ=255)   ccGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTgggg                                                                                                                                  >  1:164359/1‑140 (MQ=255)   ccGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTgggg                                                                                                                                  >  2:265684/1‑140 (MQ=255)     gTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGaa                                                                                                                                <  2:115683/140‑1 (MQ=255)       tctcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACg                                                                                                                              <  1:572450/140‑1 (MQ=255)        ctcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGa                                                                                                                             <  1:579141/140‑1 (MQ=255)         tcCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGAc                                                                                                                            >  1:346357/1‑140 (MQ=255)          ccAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGAcc                                                                                                                           <  2:32784/140‑1 (MQ=255)          ccAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGAcc                                                                                                                           <  2:569163/140‑1 (MQ=255)          ccAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGAcc                                                                                                                           <  2:111126/140‑1 (MQ=255)          ccAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGAcc                                                                                                                           <  2:687570/140‑1 (MQ=255)           cAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCt                                                                                                                          >  1:840990/1‑140 (MQ=255)           cAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCt                                                                                                                          >  2:112699/1‑140 (MQ=255)               gtgCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTa                                                                                                                      <  2:806007/140‑1 (MQ=255)               gtgCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTa                                                                                                                      <  1:780507/140‑1 (MQ=255)               gtgCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTa                                                                                                                      <  2:329812/140‑1 (MQ=255)               gtgCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTa                                                                                                                      <  1:203860/140‑1 (MQ=255)               gtgCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTa                                                                                                                      <  1:427500/140‑1 (MQ=255)                  ccATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCTGCCCGTGGGGAACGACCTGGTAtgg                                                                                                                   >  1:209837/1‑140 (MQ=255)                  ccATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTAtgg                                                                                                                   <  1:112699/140‑1 (MQ=255)                   cATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCTGTGGGGCGGCCCGTGGGGAACGACCTGGTAtggt                                                                                                                  <  2:817182/140‑1 (MQ=255)                   cATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTAtggt                                                                                                                  >  2:297540/1‑140 (MQ=255)                    aTTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTAtggtg                                                                                                                 <  2:748572/140‑1 (MQ=255)                     ttACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTAtggtgg                                                                                                                >  2:883445/1‑140 (MQ=255)                           cAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACa                                                                                                          <  2:182370/140‑1 (MQ=255)                               gTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAatat                                                                                                      >  1:489744/1‑140 (MQ=255)                                tCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATg                                                                                                     <  2:875049/140‑1 (MQ=255)                                 cAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGc                                                                                                    <  2:588799/140‑1 (MQ=255)                                     ggCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGaa                                                                                                <  1:586968/140‑1 (MQ=255)                                     ggCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGaa                                                                                                <  2:346357/140‑1 (MQ=255)                                         aaTCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATAtt                                                                                            >  1:751352/1‑140 (MQ=255)                                                   gcgGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTgccgc                                                                                  <  2:164359/140‑1 (MQ=255)                                                       tGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATg                                                                              >  2:679699/1‑140 (MQ=255)                                                         acacTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGtt                                                                            >  2:372556/1‑140 (MQ=255)                                                          cacTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGt                                                                             <  2:906570/138‑1 (MQ=255)                                                          cacTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGt                                                                             >  1:906570/1‑138 (MQ=255)                                                                         gAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAAc                                                            >  2:847614/1‑140 (MQ=255)                                                                         gAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAAc                                                            >  1:854125/1‑140 (MQ=255)                                                                               aTAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGc                                                      >  2:300592/1‑140 (MQ=255)                                                                                     aTGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTc                                                >  2:622795/1‑140 (MQ=255)                                                                                      tGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCg                                               >  2:857622/1‑140 (MQ=255)                                                                                         aCAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAg                                            <  2:252470/140‑1 (MQ=255)                                                                                         aCAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAg                                            <  2:751352/140‑1 (MQ=255)                                                                                             cagGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAAt                                        >  1:919853/1‑140 (MQ=255)                                                                                             cagGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAAt                                        >  1:360605/1‑140 (MQ=255)                                                                                                  gagCAAATTTGCCCAGGCCACGTTGCAGTGGGGCTGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATttcgt                                   <  2:209837/140‑1 (MQ=255)                                                                                                     cAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATttcgttcg                                >  2:136806/1‑140 (MQ=255)                                                                                                        aTTTGCCCAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATg                             <  1:807395/140‑1 (MQ=255)                                                                                                             cccAGGCCACGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGAcc                        >  1:440153/1‑140 (MQ=255)                                                                                                                     aCGTTGCAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGACCCAGGCGaa                <  1:622795/140‑1 (MQ=255)                                                                                                                           cAGTGGGGCGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGACCCAGGCGAAGAGTAc          <  1:679699/140‑1 (MQ=255)                                                                                                                                 ggcggcCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGACCCAGGCGAAGAGTACGCTAGc    >  2:243676/1‑140 (MQ=255)                                                                                                                                 ggcggcCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGACCCAGGCGAAGAGTACGCTAGc    >  1:260795/1‑140 (MQ=255)                                                                                                                                   cGGCCCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGACCCAGGCGAAGAGTACGCTAGCAg  >  1:914086/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         TACCGTTCTCCAGTGTGCCATTACTCCAGCGTCAGGGGCGAATCAAATACGCGGTGACACTGGCGAAATACAGAACCAATAGTAATGAACAGCAGGAGAGCAAATTTGCCCAGGCCACGTTGCAGTGGGGCGGACCGTGGGGAACGACCTGGTATGGTGGTGGACAATATGCTGAATATTACCGTGCCGCCATGTTTGGTCTGGGATTTAACCTCGGCGATTTCGGAGCAATTTCGTTCGATGCGACCCAGGCGAAGAGTACGCTAGCAG  >  NC_000913/1000255‑1000524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑