Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,102,105	A→G	V8A (GTT→GCT)	csgF ←	curli assembly component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,102,105	0	A	G	100.0%	171.2 / NA	46	V8A (GTT→GCT)	csgF	curli assembly component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/18);  total (28/18)

TTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGT  >  NC_000913/1101973‑1102239                                                                                                                                     |                                                                                                                                       ttATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGc                                                                                                                                 <  2:183404/140‑1 (MQ=255)   ataAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCAt                                                                                                                               >  1:860865/1‑140 (MQ=255)       gagTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGttt                                                                                                                           >  2:414198/1‑140 (MQ=255)          ttttGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGac                                                                                                                        <  1:414198/140‑1 (MQ=255)                    gAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCa                                                                                                              >  1:419497/1‑140 (MQ=255)                    gAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCa                                                                                                              >  1:539550/1‑140 (MQ=255)                    gAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCa                                                                                                              >  1:710355/1‑140 (MQ=255)                     aGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAg                                                                                                             >  1:782993/1‑140 (MQ=255)                            tttAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGa                                                                                                      <  1:474486/140‑1 (MQ=255)                                 taaAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGaa                                                                                                     >  1:21369/1‑136 (MQ=255)                                 taaAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGaa                                                                                                     <  2:21369/136‑1 (MQ=255)                                 taaAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAt                                                                                                 >  1:854038/1‑140 (MQ=255)                                    aaaaGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAtttt                                                                                              >  1:790039/1‑140 (MQ=255)                                      aaGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAtttttt                                                                                            >  2:768638/1‑140 (MQ=255)                                      aaGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAAtttttt                                                                                            >  1:47540/1‑140 (MQ=255)                                                        tACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATgc                                                                          >  1:877229/1‑140 (MQ=255)                                                         accaccAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATgcg                                                                         >  2:335851/1‑140 (MQ=255)                                                         accaccAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATgcg                                                                         >  1:607419/1‑140 (MQ=255)                                                         accaccAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATgcg                                                                         >  2:324206/1‑140 (MQ=255)                                                         accaccAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATgcg                                                                         >  2:611055/1‑140 (MQ=255)                                                         accaccAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATgcg                                                                         >  1:156570/1‑140 (MQ=255)                                                         accaccAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATgcg                                                                         >  1:169902/1‑140 (MQ=255)                                                               aaaGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAAt                                                                   <  2:156570/140‑1 (MQ=255)                                                                  gTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGc                                                                <  2:877229/140‑1 (MQ=255)                                                                  gTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGc                                                                <  2:892666/140‑1 (MQ=255)                                                                    ttGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGccc                                                              <  1:260899/140‑1 (MQ=255)                                                                    ttGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGccc                                                              <  2:782993/140‑1 (MQ=255)                                                                    ttGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGccc                                                              <  1:611055/140‑1 (MQ=255)                                                                    ttGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGccc                                                              <  1:335851/140‑1 (MQ=255)                                                                    ttGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGccc                                                              <  2:419497/140‑1 (MQ=255)                                                                     tGGATTACGGAACTGGAAAGTCATGGTCCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCg                                                             >  2:837259/1‑140 (MQ=255)                                                                        aTTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGc                                                                <  2:400057/134‑1 (MQ=255)                                                                        aTTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGc                                                                >  1:400057/1‑134 (MQ=255)                                                                                 cTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACg                                                 >  2:510654/1‑140 (MQ=255)                                                                                 cTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACg                                                 <  1:324206/140‑1 (MQ=255)                                                                                  tGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGc                                                <  2:860865/140‑1 (MQ=255)                                                                                                         aCTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTa                         >  1:558475/1‑140 (MQ=255)                                                                                                         aCTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTa                         >  1:845769/1‑140 (MQ=255)                                                                                                         aCTTAATGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTa                         >  2:880865/1‑140 (MQ=255)                                                                                                               tGGCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTAC‑TAATAACGCCTGATTTATCTGGCGACGATTTAGTGcttc                  >  1:868457/1‑140 (MQ=255)                                                                                                                ggCGAAATAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGcttc                  <  2:278727/140‑1 (MQ=255)                                                                                                                    aaaTAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCa              <  2:539550/140‑1 (MQ=255)                                                                                                                    aaaTAAGCATGAGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCa              >  1:829232/1‑140 (MQ=255)                                                                                                                               aGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAg   >  2:890254/1‑140 (MQ=255)                                                                                                                               aGTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAg   >  1:849430/1‑140 (MQ=255)                                                                                                                                gTAGAGCTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTTAATCAGt  <  2:47540/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TTATAAGAGTTTTGGGCCTGAGCGCTATTTAATAAAAAAGCGCCATTATTTGGGTTACCACCAAAGTTTGGATTACGGAACTGGAAAGTCATGGTTCCAGCCCAACTTAATGGCGAAATAAGCATGAGTAGAACTACTGCATGTTTGACACGCATTGCAGCCTCCGAACAATTTTTTATTTAGAATTCATCATGCGCCAAATCGCCCGTACTTAATAACGCCTGATTTATCTGGCGACGATTTAGTGCTTCTTCAGTTTGAATCAGT  >  NC_000913/1101973‑1102239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑