Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,176,268	A→G	N281S (AAC→AGC)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,176,268	0	A	G	100.0%	155.4 / NA	42	N281S (AAC→AGC)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

CAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCG  >  NC_000913/1176135‑1176404                                                                                                                                      |                                                                                                                                         cAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCattat                                                                                                                                    <  2:156147/140‑1 (MQ=255) cAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCattat                                                                                                                                    <  2:847012/140‑1 (MQ=255)  aGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCattatt                                                                                                                                   >  2:584720/1‑140 (MQ=255)    cAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTAc                                                                                                                                 >  1:340595/1‑140 (MQ=255)    cAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTAc                                                                                                                                 >  2:740173/1‑140 (MQ=255)    cAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTAc                                                                                                                                 <  1:740173/140‑1 (MQ=255)    cAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTAc                                                                                                                                 >  2:830188/1‑140 (MQ=255)                gAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCt                                                                                                                     <  1:21171/140‑1 (MQ=255)                gAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCt                                                                                                                     <  1:830188/140‑1 (MQ=255)                  aGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGa                                                                                                                   <  2:752866/140‑1 (MQ=255)                                aGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAg                                                                                                     <  1:286576/140‑1 (MQ=255)                                aGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAg                                                                                                     <  2:340595/140‑1 (MQ=255)                                     tGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAggg                                                                                                <  1:8455/140‑1 (MQ=255)                                     tGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAggg                                                                                                <  1:801006/140‑1 (MQ=255)                                        cGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGa                                                                                             <  2:434785/140‑1 (MQ=255)                                                        aGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCaa                                                                             >  2:653311/1‑140 (MQ=255)                                                        aGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCaa                                                                             >  1:711845/1‑140 (MQ=255)                                                         gttgttCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCaaa                                                                            >  1:616877/1‑140 (MQ=255)                                                                  ggCCGTACACATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCt                                                                   >  1:342650/1‑140 (MQ=255)                                                                   gCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCtt                                                                  <  1:866330/140‑1 (MQ=255)                                                                     cGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTaa                                                                >  1:244797/1‑140 (MQ=255)                                                                       tACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACt                                                              >  2:322114/1‑140 (MQ=255)                                                                       tACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACt                                                              >  2:573020/1‑140 (MQ=255)                                                                       tACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACt                                                              >  1:308106/1‑140 (MQ=255)                                                                        aCGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAAGCTTAACTc                                                             >  1:638276/1‑140 (MQ=255)                                                                                 aaaaaaTATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACaaa                                                    <  2:418289/140‑1 (MQ=255)                                                                                      atatGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatg                                               <  2:711845/140‑1 (MQ=255)                                                                                      atatGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCatg                                               <  2:308106/140‑1 (MQ=255)                                                                                               gTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCttt                                      >  1:577799/1‑140 (MQ=255)                                                                                                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTa                                     >  1:866795/1‑140 (MQ=255)                                                                                                tttACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTa                                     >  1:158013/1‑140 (MQ=255)                                                                                                         gAGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAg                            >  1:878691/1‑140 (MQ=255)                                                                                                          aGCCTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAgg                           >  1:412034/1‑140 (MQ=255)                                                                                                            ccTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAgggg                         >  1:678374/1‑140 (MQ=255)                                                                                                            ccTGATTGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAgggg                         >  1:592160/1‑140 (MQ=255)                                                                                                                 ttGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgc                    >  2:618371/1‑140 (MQ=255)                                                                                                                 ttGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgc                    >  2:620588/1‑140 (MQ=255)                                                                                                                  tGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcg                   <  2:866795/140‑1 (MQ=255)                                                                                                                  tGTCGCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAgcg                   <  2:158013/140‑1 (MQ=255)                                                                                                                      gCCGTTGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCgg               >  1:182018/1‑140 (MQ=255)                                                                                                                           tGCGGCGTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTa          >  1:61139/1‑140 (MQ=255)                                                                                                                                cgTTTAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGt     <  2:616877/140‑1 (MQ=255)                                                                                                                                   ttAGCATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCg  <  1:733951/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTCCAGGCCGTACGCATGGAAAAAAATATGATGGGTTTACTGCTGAGCCTGATTGTCGCCGTTGCGGCGTTTAACATTATTACCTCACTAGGGCTGATGGTAATGGAGAAGCAGGGCGAAGTAGCGATCCTGCAAACGCAAGGCTTAACTCCGCGACAAATCATGATGGTCTTTATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCG  >  NC_000913/1176135‑1176404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑