Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,220,971	G→A	pseudogene (1371/2648 nt)	ycgH →	putative transporter component, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,220,971	0	G	A	100.0%	328.8 / NA	91	pseudogene (1371/2648 nt)	ycgH	putative transporter component, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/40);  total (51/40)

AATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCCAGCAG  >  NC_000913/1220834‑1221107                                                                                                                                            |                                                                                                                                         aaTTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGc                                                                                                                                        <  2:514365/140‑1 (MQ=255)  aTTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCt                                                                                                                                       <  2:531297/140‑1 (MQ=255)  aTTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCt                                                                                                                                       <  1:242753/140‑1 (MQ=255)   ttCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTa                                                                                                                                      >  2:220047/1‑140 (MQ=255)       gACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGa                                                                                                                                  <  2:868611/140‑1 (MQ=255)       gACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGa                                                                                                                                  <  1:736415/140‑1 (MQ=255)            tattatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGAc                                                                                                                             >  2:122304/1‑140 (MQ=255)            tattatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGAc                                                                                                                             >  1:849126/1‑140 (MQ=255)             attatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACt                                                                                                                            >  2:666754/1‑140 (MQ=255)              ttatCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACtt                                                                                                                           >  1:532272/1‑140 (MQ=255)                    aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                                     >  2:718627/1‑140 (MQ=255)                    aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                                     <  2:688717/140‑1 (MQ=255)                    aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                                     <  1:297098/140‑1 (MQ=255)                    aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                                     <  1:122304/140‑1 (MQ=255)                    aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                                     <  2:87963/140‑1 (MQ=255)                    aTCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                                     >  1:256273/1‑140 (MQ=255)                    aTCTCTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTAttgtt                                                                                                                     <  2:849126/140‑1 (MQ=255)                      cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                                   >  1:179757/1‑140 (MQ=255)                      cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                                   >  1:278764/1‑140 (MQ=255)                      cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                                   >  1:845379/1‑140 (MQ=255)                      cTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGa                                                                                                                   >  1:647453/1‑140 (MQ=255)                        ttCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAgg                                                                                                                 >  1:315127/1‑140 (MQ=255)                         tCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAggg                                                                                                                <  2:532272/140‑1 (MQ=255)                         tCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAggg                                                                                                                >  2:249212/1‑140 (MQ=255)                               gatgatgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATAc                                                                                                          >  2:571361/1‑140 (MQ=255)                                 tgatgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACtt                                                                                                        <  2:647453/140‑1 (MQ=255)                                   atgGTTAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCg                                                                                                      <  1:244252/140‑1 (MQ=255)                                   atgGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCg                                                                                                      <  2:256273/140‑1 (MQ=255)                                      gTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGt                                                                                                   >  1:351155/1‑140 (MQ=255)                                      gTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGt                                                                                                   >  1:740949/1‑140 (MQ=255)                                       tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                  <  1:673711/140‑1 (MQ=255)                                       tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                  >  1:697581/1‑140 (MQ=255)                                       tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                  >  1:742276/1‑140 (MQ=255)                                       tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                  <  2:713200/140‑1 (MQ=255)                                       tCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                  <  2:179757/140‑1 (MQ=255)                                         aaaCATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCa                                                                                                >  2:685149/1‑140 (MQ=255)                                          aaCATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCaa                                                                                               >  2:132016/1‑140 (MQ=255)                                           aCATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCa                                                                                                >  1:465723/1‑140 (MQ=255)                                           aCATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTAAAc                                                                                              >  2:354665/1‑140 (MQ=255)                                                tACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCg                                                                                         >  1:283880/1‑140 (MQ=255)                                                 aCCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGt                                                                                        >  2:820189/1‑140 (MQ=255)                                                      aGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTAtg                                                                                   >  1:753825/1‑140 (MQ=255)                                                       ggAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGGTGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                  >  2:570415/1‑124 (MQ=255)                                                       ggAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGtt                                                                                                  <  1:570415/124‑1 (MQ=255)                                                       ggAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTAtgt                                                                                  >  1:238854/1‑140 (MQ=255)                                                       ggAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTAtgt                                                                                  >  1:516511/1‑140 (MQ=255)                                                          aaaaCATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGaa                                                                               <  1:718627/140‑1 (MQ=255)                                                                 tAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCt                                                                        >  2:513773/1‑140 (MQ=255)                                                                 tAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCt                                                                        <  2:753825/140‑1 (MQ=255)                                                                 tAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCt                                                                        >  1:295860/1‑140 (MQ=255)                                                                  aaCCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTg                                                                       >  2:147217/1‑140 (MQ=255)                                                                   aCCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTgg                                                                      >  2:739582/1‑140 (MQ=255)                                                                              tAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcg                                                           <  2:845379/140‑1 (MQ=255)                                                                               aaTTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgc                                                          >  1:839429/1‑140 (MQ=255)                                                                                aTTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcg                                                         >  2:132635/1‑140 (MQ=255)                                                                                    tGTCGGTAATAATGGTCAGCTTAATATCCGCACCGTGTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAgcggcgcggc                                                       <  2:465723/140‑1 (MQ=255)                                                                                      tCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACg                                                   <  1:513773/140‑1 (MQ=255)                                                                                      tCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACg                                                   <  2:315127/140‑1 (MQ=255)                                                                                       cGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGc                                                  >  1:225277/1‑140 (MQ=255)                                                                                          taataaTGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTaa                                               >  1:114650/1‑140 (MQ=255)                                                                                           aataatGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTaaa                                              <  2:65349/140‑1 (MQ=255)                                                                                                tGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGa                                         <  1:147217/140‑1 (MQ=255)                                                                                                  gTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATc                                       <  1:685149/140‑1 (MQ=255)                                                                                                    cAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGa                                     >  1:899943/1‑140 (MQ=255)                                                                                                    cAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGa                                     >  1:187692/1‑140 (MQ=255)                                                                                                    cAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGa                                     <  2:351155/140‑1 (MQ=255)                                                                                                       cTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                                  <  2:899943/140‑1 (MQ=255)                                                                                                       cTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                                  >  2:844664/1‑140 (MQ=255)                                                                                                       cTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                                  >  1:520267/1‑140 (MQ=255)                                                                                                       cTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACt                                  <  1:820189/140‑1 (MQ=255)                                                                                                          aaTATCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCAt                               <  1:724463/140‑1 (MQ=255)                                                                                                           atatCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATa                              <  1:391056/140‑1 (MQ=255)                                                                                                            tatCCGCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATaa                             <  2:735246/140‑1 (MQ=255)                                                                                                                 gCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                        >  1:355887/1‑140 (MQ=255)                                                                                                                 gCACC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACtgtg                        >  2:167845/1‑140 (MQ=255)                                                                                                                   aCC‑‑GTATTAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGaa                      <  1:844664/140‑1 (MQ=255)                                                                                                                            tAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGAt               <  2:742276/140‑1 (MQ=255)                                                                                                                            tAGGTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGAt               <  2:839429/140‑1 (MQ=255)                                                                                                                              ggTGATGATAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGa             <  1:220047/140‑1 (MQ=255)                                                                                                                                tgatgaTAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAAC‑CATAACTGTGAATGGCGATGAAtc          >  2:513258/1‑140 (MQ=255)                                                                                                                                 gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGa             >  1:748633/1‑137 (MQ=255)                                                                                                                                 gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGa             <  2:748633/137‑1 (MQ=255)                                                                                                                                 gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc          >  1:817455/1‑140 (MQ=255)                                                                                                                                 gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc          >  1:563744/1‑140 (MQ=255)                                                                                                                                 gatgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtc          >  1:615332/1‑140 (MQ=255)                                                                                                                                  atgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtct         >  2:45577/1‑140 (MQ=255)                                                                                                                                   tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAAtctc        >  1:842356/1‑140 (MQ=255)                                                                                                                                   tgatAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGAAGAAtctc        >  2:601157/1‑140 (MQ=255)                                                                                                                                     atAAATCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCca      >  2:811478/1‑140 (MQ=255)                                                                                                                                        aaTCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCcagca   <  2:43249/140‑1 (MQ=255)                                                                                                                                         aTCAGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCcagcag  <  2:520267/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                         AATTCAGACAGTATTATCAATCTTTCGTATGATGATGGTCAAACATTTACCCAAGGAAAAACATTAACCGTAAAAGGTAATTATGTCGGTAATAATGGTCAGCTTAATATCCGCACC‑‑GTATTAGGTGATGATAAATCGGCTACGGACAGACTTATTGTTGAGGGTAATACTTCGGGTTCAACTACCGTCTATGTGAAAAATGCTGGAGGAAGCGGCGCGGCCACGCTAAACGGGATCGAACTCATAACTGTGAATGGCGATGAATCTCCAGCAG  >  NC_000913/1220834‑1221107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑