Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,256,814	T→C	E333E (GAA→GAG)	ychF ←	redox‑responsive ATPase YchF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,256,814	0	T	C	100.0%	197.8 / NA	53	E333E (GAA→GAG)	ychF	redox‑responsive ATPase YchF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/24);  total (29/24)

GCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGTTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCACT  >  NC_000913/1256677‑1256946                                                                                                                                          |                                                                                                                                     gcTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTTCCTGCTTCTTTCGCGCCTTGCTc                                                                                                                                    <  2:561770/140‑1 (MQ=255)  ctctTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCa                                                                                                                                   <  1:399678/140‑1 (MQ=255)     ttCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCt                                                                                                                                >  1:223942/1‑140 (MQ=255)       cATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGACTTGCTc                                                                                                                                    >  1:489645/1‑134 (MQ=255)         tGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGt                                                                                                                            <  1:16569/140‑1 (MQ=255)         tGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGt                                                                                                                            <  1:875376/140‑1 (MQ=255)         tGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTCTCGCGCCTTGCTCACCTTTGt                                                                                                                            <  2:654837/140‑1 (MQ=255)           agaTTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTaa                                                                                                                          <  1:618531/140‑1 (MQ=255)                 aGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCCCGCCTTGCTCACCTTTGTAAGtgatg                                                                                                                    >  1:851498/1‑140 (MQ=255)                   cGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGaa                                                                                                                  >  2:431292/1‑140 (MQ=255)                       aCCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCt                                                                                                              <  1:563422/140‑1 (MQ=255)                       aCCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCt                                                                                                              >  1:213570/1‑140 (MQ=255)                         cTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTc                                                                                                            <  2:213570/140‑1 (MQ=255)                            aTGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCaaa                                                                                                         >  2:554153/1‑140 (MQ=255)                                 aCAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACgaga                                                                                                    <  1:887427/140‑1 (MQ=255)                                   aaTAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACgaga                                                                                                    <  2:243120/138‑1 (MQ=255)                                   aaTAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACgaga                                                                                                    >  1:243120/1‑138 (MQ=255)                                     tAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGAt                                                                                                   >  2:555440/1‑137 (MQ=255)                                     tAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGAt                                                                                                   <  1:555440/137‑1 (MQ=255)                                          taattaGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTtgtg                                                                                           >  2:229774/1‑140 (MQ=255)                                           aattaGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGc                                                                                          >  1:29561/1‑140 (MQ=255)                                             ttaGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCAc                                                                                        >  1:426103/1‑140 (MQ=255)                                               aGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACgg                                                                                      >  2:844543/1‑140 (MQ=255)                                                 aCGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGt                                                                                                >  1:380514/1‑128 (MQ=255)                                                 aCGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGt                                                                                                <  2:380514/128‑1 (MQ=255)                                                        aaaGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCt                                                                             <  1:899181/140‑1 (MQ=255)                                                             aaGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTc                                                                        >  2:813976/1‑140 (MQ=255)                                                                ttCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCaaa                                                                     <  1:554153/140‑1 (MQ=255)                                                                   atcaCATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATc                                                                  <  1:229774/140‑1 (MQ=255)                                                                          cGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATgg                                                           >  1:502975/1‑140 (MQ=255)                                                                                    cACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCg                                                 >  1:74415/1‑140 (MQ=255)                                                                                                   aCCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCgg                                  >  2:166512/1‑140 (MQ=255)                                                                                                   aCCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCgg                                  >  1:524497/1‑140 (MQ=255)                                                                                                   aCCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCgg                                  >  1:631963/1‑140 (MQ=255)                                                                                                   aCCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCgg                                  <  1:691201/140‑1 (MQ=255)                                                                                                     cTTCTGCACGCATTTTGCCTGCTTCTCTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGtt                                >  2:196827/1‑140 (MQ=255)                                                                                                        cTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCt                             >  2:922147/1‑140 (MQ=255)                                                                                                         tGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTc                            >  1:638270/1‑140 (MQ=255)                                                                                                         tGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTc                            >  1:194498/1‑140 (MQ=255)                                                                                                         tGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGCTCAACTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAGTCAGTATGGATTTGGCCCGCTGCCTGCGGCGCGGGTGCTc                            >  2:815927/1‑140 (MQ=255)                                                                                                                aTTTTGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCgg                     >  2:285850/1‑140 (MQ=255)                                                                                                                 ttttGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGa                    <  2:271097/140‑1 (MQ=255)                                                                                                                  tttGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGaa                   <  2:524497/140‑1 (MQ=255)                                                                                                                  tttGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGaa                   <  1:431292/140‑1 (MQ=255)                                                                                                                    tGCCTGCTTCTTTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATg                 <  2:194498/140‑1 (MQ=255)                                                                                                                          cttcttTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCAt           >  1:369814/1‑140 (MQ=255)                                                                                                                          cttcttTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCAt           >  2:26805/1‑140 (MQ=255)                                                                                                                          cttcttTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCAt           >  1:119480/1‑140 (MQ=255)                                                                                                                            tcttTCGCGCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATgc         >  1:906041/1‑140 (MQ=255)                                                                                                                                 cgcgCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATgcacgca    <  2:180519/140‑1 (MQ=255)                                                                                                                                 cgcgCCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATgcacgca    <  1:166512/140‑1 (MQ=255)                                                                                                                                   cgcCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCAct  <  1:576669/140‑1 (MQ=255)                                                                                                                                   cgcCTTGCTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCAct  <  1:196827/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     GCTCTTCATGAGATTTAGCGAAACCTCATGAGACAATAAATTAATTAGACGTTGAAAAGGAAGTTCATCACATCGCCATCTTTCACGATGTAATCTTTACCTTCTGCACGCATTTTGCCTGCTTCTTTCGCGCCTTGTTCACCTTTGTAAGTGATGAAATCTTCAAACGAGATGGTTTGTGCACGGATAAAGCCTTTTTCAAAATCAGTATGGATTTTGCCCGCTGCCTGCGGCGCGGTTGCTCCAACCGGAATGGTCCATGCACGCACT  >  NC_000913/1256677‑1256946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑