Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,276,807	A→G	V271A (GTA→GCA)	narX ←	sensory histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,276,807	0	A	G	100.0%	178.2 / NA	49	V271A (GTA→GCA)	narX	sensory histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/17);  total (32/17)

GGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTACAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAA  >  NC_000913/1276670‑1276941                                                                                                                                          |                                                                                                                                       ggCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCa                                                                                                                                      <  2:82919/140‑1 (MQ=255)      agCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGa                                                                                                                                 <  2:399921/140‑1 (MQ=255)      agCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGa                                                                                                                                 <  2:448481/140‑1 (MQ=255)             aGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGt                                                                                                                          >  2:617789/1‑140 (MQ=255)             aGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGt                                                                                                                          <  1:617789/140‑1 (MQ=255)                cTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTca                                                                                                                       <  2:844789/140‑1 (MQ=255)                          acaAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCgggg                                                                                                             <  1:453354/140‑1 (MQ=255)                          acaAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCgggg                                                                                                             >  1:52329/1‑140 (MQ=255)                             aGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGgcgc                                                                                                          >  2:486703/1‑140 (MQ=255)                               tCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCgg                                                                                                        >  1:643704/1‑140 (MQ=255)                                cATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCggg                                                                                                       <  2:643704/140‑1 (MQ=255)                                cATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCggg                                                                                                       <  2:462878/140‑1 (MQ=255)                                         ctggcAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAAc                                                                                              >  1:478166/1‑140 (MQ=255)                                            gcAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGgcg                                                                                                           >  1:319041/1‑124 (MQ=255)                                            gcAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGgcg                                                                                                           <  2:319041/124‑1 (MQ=255)                                             cAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAAcggcg                                                                                          >  1:69432/1‑140 (MQ=255)                                                          gatgatTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCAt                                                                             >  2:239783/1‑140 (MQ=255)                                                              atTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATaaaa                                                                         <  1:486703/140‑1 (MQ=255)                                                                tctcTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGTGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATaaaaaa                                                                       >  2:292804/1‑140 (MQ=255)                                                                tctcTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATaaaaaa                                                                       >  2:864269/1‑140 (MQ=255)                                                                  tctTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAaga                                                                     <  1:864269/140‑1 (MQ=255)                                                                     tcatcatcaGTGTCATACACCCGCAATTCGACATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAagagag                                                                  >  2:338821/1‑140 (MQ=255)                                                                              gtgtCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGAtt                                                         >  2:897467/1‑140 (MQ=255)                                                                              gtgtCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGAtt                                                         >  1:749311/1‑140 (MQ=255)                                                                                  cATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTa                                                     <  2:52329/140‑1 (MQ=255)                                                                                      cacCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAACCTTCCCTAAAAAAGAGAGGATCTGATTTTTATGCt                                                 >  2:596661/1‑140 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTa                                                     >  2:289970/1‑133 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTa                                                     <  1:289970/133‑1 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTccc                                              >  2:501421/1‑139 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCa                                              >  2:246774/1‑140 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCa                                              >  2:406965/1‑140 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCa                                              >  2:338423/1‑140 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCa                                              >  1:900622/1‑140 (MQ=255)                                                                                         ccGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCa                                              >  1:795334/1‑140 (MQ=255)                                                                                                 cGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCcggcg                                      <  1:880756/140‑1 (MQ=255)                                                                                                   atatCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCcggcggg                                    >  2:567723/1‑139 (MQ=255)                                                                                                        ccGTAGCAGGTTAAAATTCTGTAAGCCTTTGAGTGCGGGTGACGGGCGTCCACACGGGGGGGCGCGGAAATCAAACCGGGGTTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTtct                               <  2:177849/139‑1 (MQ=255)                                                                                                         cGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTtctc                              >  1:903180/1‑140 (MQ=255)                                                                                                         cGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTtctc                              >  1:185739/1‑140 (MQ=255)                                                                                                         cGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGGTTtttc                              >  2:792583/1‑140 (MQ=255)                                                                                                           tAGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGAACTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCt                            >  1:631355/1‑140 (MQ=255)                                                                                                            aGCAGGGTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTg                           >  2:708581/1‑140 (MQ=255)                                                                                                                gggTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAAcc                       <  2:3564/140‑1 (MQ=255)                                                                                                                 ggTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGGTTTCTCCTGAAccc                      >  1:177849/1‑140 (MQ=255)                                                                                                                  gTTAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCg                     <  1:147253/140‑1 (MQ=255)                                                                                                                   ttAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATGTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAAgcccc                    >  2:849279/1‑138 (MQ=255)                                                                                                                   ttAAATTCTGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCgc                    <  1:596661/140‑1 (MQ=255)                                                                                                                           tGTAAGCCGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGGTTTCTCCTGAACCCGCTGCTCaaa            >  2:477166/1‑139 (MQ=255)                                                                                                                                  cGTTGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGA‑TTTTATGCTCCAGCCCGGCGGGTTTTTCCTGAAACCGCTGCTCAAGTACGGCAt    >  2:98311/1‑140 (MQ=255)                                                                                                                                     tGAGTGCAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGGTTTCTCCTGAACCCGCCGCTCAAGTACAGCATaa  >  2:751550/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTACAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAAACGGCGGTTAGCCTGCCATAAAAAAGAGAGGATCTGATTTTTATGCTCCAGCCCGGCGGTTTTCTCCTGAACCCGCTGCTCAAGTACGGCATAA  >  NC_000913/1276670‑1276941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑