Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,338,450	A→G	T874A (ACC→GCC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,338,450	0	A	G	100.0%	159.8 / NA	44	T874A (ACC→GCC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/21);  total (23/21)

CCGGGGAAGAGAAGATTGATATTGGCGATCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGACCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGTTATTTGTTC  >  NC_000913/1338313‑1338579                                                                                                                                          |                                                                                                                                  ccGGGGAAGAGAAGATTGATATTGGCGATCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGcc                                                                                                                                 <  1:797105/140‑1 (MQ=255)          agaagaTTGATATTGGCGATCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAg                                                                                                                        >  1:731495/1‑140 (MQ=255)                    tatTGGCGATCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCa                                                                                                              >  2:922666/1‑140 (MQ=255)                             tCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCa                                                                                                       <  2:768800/138‑1 (MQ=255)                             tCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCa                                                                                                       >  1:768800/1‑138 (MQ=255)                             tCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCAtt                                                                                                     <  1:911550/140‑1 (MQ=255)                                    aaCCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCAt                                                                                              <  1:493763/140‑1 (MQ=255)                                         aCAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTa                                                                                         >  1:541542/1‑140 (MQ=255)                                         aCAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTa                                                                                         <  2:624340/140‑1 (MQ=255)                                         aCAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTa                                                                                         >  1:314541/1‑140 (MQ=255)                                          cAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTaa                                                                                        <  2:161111/140‑1 (MQ=255)                                                ggCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGtt                                                                                  >  1:99471/1‑140 (MQ=255)                                                  cgcgACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGa                                                                                >  2:128721/1‑140 (MQ=255)                                                     gACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGt                                                                             <  1:796700/140‑1 (MQ=255)                                                       cGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGtt                                                                                  <  2:220711/133‑1 (MQ=255)                                                       cGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGtt                                                                                  >  1:220711/1‑133 (MQ=255)                                                       cGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTaa                                                                           <  2:302158/140‑1 (MQ=255)                                                        ggTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTaac                                                                          <  1:128721/140‑1 (MQ=255)                                                        ggTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTaac                                                                          <  2:731495/140‑1 (MQ=255)                                                           tCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGa                                                                                <  1:101772/131‑1 (MQ=255)                                                           tCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGa                                                                                >  2:101772/1‑131 (MQ=255)                                                           tCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTaac                                                                          <  2:490135/137‑1 (MQ=255)                                                           tCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTaac                                                                          >  1:490135/1‑137 (MQ=255)                                                                    gCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATttgctt                                                              >  2:678101/1‑140 (MQ=255)                                                                    gCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATttgctt                                                              <  1:678101/140‑1 (MQ=255)                                                                          gcgcgcgGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGt                                                        >  1:445704/1‑140 (MQ=255)                                                                                    ggTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCtt                                              >  1:443942/1‑140 (MQ=255)                                                                                               aGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGgcaagcaa                                   <  2:99471/140‑1 (MQ=255)                                                                                                gTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCaaa                                  >  2:397054/1‑140 (MQ=255)                                                                                                  cGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATg                                >  1:767668/1‑140 (MQ=255)                                                                                                         tGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTaca                         <  1:791524/140‑1 (MQ=255)                                                                                                         tGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTaca                         <  2:541542/140‑1 (MQ=255)                                                                                                         tGCCGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTaca                         >  2:791524/1‑140 (MQ=255)                                                                                                           ccGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTaca                         >  2:149925/1‑138 (MQ=255)                                                                                                           ccGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTaca                         <  1:149925/138‑1 (MQ=255)                                                                                                           ccGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTAcaca                       >  2:143561/1‑140 (MQ=255)                                                                                                           ccGTTGTCGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTAcaca                       >  1:701341/1‑140 (MQ=255)                                                                                                                  cGTATCGACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTCGCTTCCGGCAAGCAAATGAATTACACAATGCAag                <  2:817874/140‑1 (MQ=255)                                                                                                                        gACACCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGtt          <  1:143561/140‑1 (MQ=255)                                                                                                                          cacCGCGACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGTTAt        <  2:443942/140‑1 (MQ=255)                                                                                                                             cgcgACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGTTATTTg     >  2:571992/1‑140 (MQ=255)                                                                                                                               cgACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGTTATTTGtt   >  2:241587/1‑140 (MQ=255)                                                                                                                               cgACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGTTATTTGtt   >  1:859728/1‑140 (MQ=255)                                                                                                                                gACGGAGTTGGCCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGTTATTTGTTc  >  1:651995/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  CCGGGGAAGAGAAGATTGATATTGGCGATCTGCAAAACCTACAACCCGGCGCGACGGTTCCGGTGACGCTTACGCGCGCGGATGGTAGCCAGGAAGTCGTACCCTGCCGTTGTCGTATCGACACCGCGACGGAGTTGACCTACTACCAGAACGACGGCATTTTGCATTATGTCATTCGTAATATGTTGAAGTAACAACTATTTGCTTGCCGGTTATTTTGCTTCCGGCAAGCAAATGAATTACACAATGCAAGAGGGTTATTTGTTC  >  NC_000913/1338313‑1338579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑