Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,634,602	T→C	E95G (GAA→GGA)	tfaQ ←	Qin prophage; putative tail fiber assembly protein TfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,602	0	T	C	100.0%	84.1 / NA	40	E95G (GAA→GGA)	tfaQ	Qin prophage; putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/19);  total (21/19)

TCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAG  >  NC_000913/1634510‑1634731                                                                                             |                                                                                                                                  tCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGcc                                                                                    <  2:904599/140‑1 (MQ=17) accGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGcc                                                                                    <  2:905411/139‑1 (MQ=12)   cGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCAc                                                                                  <  2:407314/140‑1 (MQ=16)          gTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATaa                                                                           >  2:22154/1‑140 (MQ=17)                cTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGtt                                                                     <  1:747247/140‑1 (MQ=16)                      ccGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTAccc                                                               >  2:813187/1‑140 (MQ=17)                          aTCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgat                                                           <  2:382112/140‑1 (MQ=16)                                cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTAccc                                                               >  2:625560/1‑130 (MQ=17)                                cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTAccc                                                               <  1:625560/130‑1 (MQ=16)                                cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCtt                                                     >  2:248574/1‑140 (MQ=17)                                 aCCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTc                                                    >  2:107700/1‑140 (MQ=17)                                      ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACga                                               >  2:917728/1‑140 (MQ=17)                                      ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACga                                               >  2:627885/1‑140 (MQ=17)                                      ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACga                                               >  2:124404/1‑140 (MQ=17)                                        cTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACgaga                                             >  2:641274/1‑140 (MQ=17)                                          gtgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg                                           >  2:114203/1‑140 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                          <  1:248574/140‑1 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                          >  1:299994/1‑140 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                          >  1:393727/1‑140 (MQ=17)                                           tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                          >  2:613071/1‑140 (MQ=17)                                             ccGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCa                                        >  1:887931/1‑140 (MQ=17)                                             ccGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCa                                        >  2:342626/1‑140 (MQ=17)                                                ttCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGa                                     <  1:331350/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                             <  1:876031/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                             <  1:228719/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                             <  2:299994/140‑1 (MQ=17)                                                        cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                             >  1:441639/1‑140 (MQ=12)                                                          gAAACTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatca                           <  1:114203/140‑1 (MQ=17)                                                             aCTCCCCTTCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTg                        <  2:577641/140‑1 (MQ=17)                                                                    ttCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACt                                <  2:291524/125‑1 (MQ=17)                                                                    ttCCGGCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACt                                >  1:291524/1‑125 (MQ=12)                                                                        ggCGATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGc             >  2:756000/1‑140 (MQ=12)                                                                           gATAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCAc          >  1:530006/1‑140 (MQ=12)                                                                            aTAACCAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACg         >  2:507615/1‑140 (MQ=12)                                                                                ccAGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagc     <  2:441639/140‑1 (MQ=17)                                                                                  aGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcca   <  1:627885/140‑1 (MQ=17)                                                                                  aGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcca   <  1:756000/140‑1 (MQ=17)                                                                                  aGGTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCACAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcca   <  1:756814/140‑1 (MQ=12)                                                                                   ggTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccag  <  2:544786/140‑1 (MQ=17)                                                                                   ggTAACATTTCCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccag  >  2:106884/1‑140 (MQ=17)                                                                                             |                                                                                                                                  TCCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAG  >  NC_000913/1634510‑1634731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑