Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,659,652	T→C	I528I (ATT→ATC)	ynfE →	putative selenate reductase YnfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,659,652	0	T	C	100.0%	163.0 / NA	44	I528I (ATT→ATC)	ynfE	putative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/26);  total (18/26)

TCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATTATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCAAA  >  NC_000913/1659516‑1659788                                                                                                                                         |                                                                                                                                         tCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATatcatt                                                                                                                                       >  2:186836/1‑140 (MQ=255) tCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATatcatt                                                                                                                                       >  1:695262/1‑140 (MQ=255)  caacaaAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTc                                                                                                                                      <  1:128355/140‑1 (MQ=255)   aacaaAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTcc                                                                                                                                     >  2:867721/1‑140 (MQ=255)           gCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACt                                                                                                                             <  1:867721/140‑1 (MQ=255)             aTGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTAc                                                                                                                           >  1:361442/1‑140 (MQ=255)                aaaTTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGcc                                                                                                                        <  2:225109/140‑1 (MQ=255)                  aTTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCgg                                                                                                                      <  2:433913/140‑1 (MQ=255)                  aTTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCgg                                                                                                                      <  2:382859/140‑1 (MQ=255)                     cTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTaa                                                                                                                   <  1:769801/140‑1 (MQ=255)                      tGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAAc                                                                                                                  >  2:732238/1‑140 (MQ=255)                      tGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAAc                                                                                                                  <  2:437447/140‑1 (MQ=255)                           gACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATggg                                                                                                             >  1:605068/1‑140 (MQ=255)                           gACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATggg                                                                                                             >  1:714299/1‑140 (MQ=255)                           gACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATggg                                                                                                             >  2:413362/1‑140 (MQ=255)                              gAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGata                                                                                                          <  1:321682/140‑1 (MQ=255)                                aTCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATAtc                                                                                                        >  2:200033/1‑140 (MQ=255)                                   gaaatgcgaaatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCa                                                                                                     <  2:714299/140‑1 (MQ=255)                                     aatgcgaaatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCAtt                                                                                                   >  1:594698/1‑140 (MQ=255)                                       tgcgaaatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCAtttt                                                                                                 <  1:732238/140‑1 (MQ=255)                                          gaaatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTtcct                                                                                              <  1:413362/140‑1 (MQ=255)                                            aatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTtcctcc                                                                                            <  2:584693/140‑1 (MQ=255)                                            aatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTtcctcc                                                                                            <  2:761200/140‑1 (MQ=255)                                            aatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTtcctcc                                                                                            <  1:530585/140‑1 (MQ=255)                                            aatgATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTtcctcc                                                                                            <  1:781280/140‑1 (MQ=255)                                                  tgtgGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTg                                                                                      >  1:73138/1‑140 (MQ=255)                                                                     aTGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGa                                                                   >  2:18385/1‑140 (MQ=255)                                                                                      atatGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACt                                                  <  2:222803/140‑1 (MQ=255)                                                                                            cGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATcc                                            >  2:307107/1‑140 (MQ=255)                                                                                                            gACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGaa                            <  2:361442/140‑1 (MQ=255)                                                                                                            gACCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGaa                            <  2:594698/140‑1 (MQ=255)                                                                                                             aCCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGaaa                           <  2:695262/140‑1 (MQ=255)                                                                                                             aCCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGaaa                           <  1:186836/140‑1 (MQ=255)                                                                                                             aCCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGaaa                           >  1:873774/1‑140 (MQ=255)                                                                                                             aCCTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGaaa                           <  1:808069/140‑1 (MQ=255)                                                                                                              ccTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGGTATCTCATTTTCCTCCAGCCTGTCACCAGCGGAAAATTCGAACGCAAACCGATTTACTGGATCCTAAGTGAAGTCGCGAAAc                          >  2:826184/1‑140 (MQ=255)                                                                                                              ccTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAAc                          >  2:239625/1‑140 (MQ=255)                                                                                                              ccTGATGACCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAAc                          >  2:892731/1‑140 (MQ=255)                                                                                                                tgatgaCCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGt                        <  2:368623/140‑1 (MQ=255)                                                                                                                  atgaCCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCt                      >  1:564035/1‑140 (MQ=255)                                                                                                                  atgaCCGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCt                      <  1:335495/140‑1 (MQ=255)                                                                                                                       cGTTGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGAc                 >  1:44283/1‑140 (MQ=255)                                                                                                                         ttGAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGACCt               >  2:866206/1‑140 (MQ=255)                                                                                                                           gAGCAGGAAGATATCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGACCTGa             <  2:605068/140‑1 (MQ=255)                                                                                                                                     atatCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCaa   <  2:564035/140‑1 (MQ=255)                                                                                                                                     atatCATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCaa   <  1:892731/140‑1 (MQ=255)                                                                                                                                      tatcatTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCaaa  >  2:888552/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         TCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGACCTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACCGTTGAGCAGGAAGATATTATTCCTAACGACTACGCCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTGAGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCAAA  >  NC_000913/1659516‑1659788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑