Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,734,085	T→C	E6G (GAA→GGA)	grxD ←	glutaredoxin 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,734,085	0	T	C	100.0%	136.3 / NA	37	E6G (GAA→GGA)	grxD	glutaredoxin 4
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/20);  total (17/20)

ATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATT  >  NC_000913/1733949‑1734216                                                                                                                                         |                                                                                                                                    atCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGa                                                                                                                                  <  1:813867/140‑1 (MQ=255)        tAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAgtggt                                                                                                                           <  1:780086/140‑1 (MQ=255)          ggCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGc                                                                                                                         >  2:305573/1‑140 (MQ=255)          ggCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGc                                                                                                                         >  2:226458/1‑140 (MQ=255)          ggCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGc                                                                                                                         >  2:187799/1‑140 (MQ=255)           gCAAAACGTTCGCCACGTGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCt                                                                                                                        >  1:224135/1‑140 (MQ=255)           gCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCt                                                                                                                        <  1:187799/140‑1 (MQ=255)           gCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCt                                                                                                                        >  1:775127/1‑140 (MQ=255)                aCGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTg                                                                                                                   >  2:671386/1‑140 (MQ=255)                   ttCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCt                                                                                                                >  1:876622/1‑140 (MQ=255)                     cGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTg                                                                                                              <  2:60316/140‑1 (MQ=255)                     cGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTg                                                                                                              <  1:654996/140‑1 (MQ=255)                      gCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGc                                                                                                             <  1:33180/140‑1 (MQ=255)                                gCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAAc                                                                                                   <  2:625183/140‑1 (MQ=255)                                   aGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAActtc                                                                                                <  1:788833/140‑1 (MQ=255)                                       ccTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTa                                                                                            <  2:224135/140‑1 (MQ=255)                                       ccTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTa                                                                                            <  1:253824/140‑1 (MQ=255)                                           gACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGc                                                                                        <  2:775127/140‑1 (MQ=255)                                                ccTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTaa                                                                                   >  2:482698/1‑140 (MQ=255)                                                ccTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTaa                                                                                   >  2:796879/1‑140 (MQ=255)                                                       agagAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACa                                                                            <  1:796879/140‑1 (MQ=255)                                                        gagaAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTAc                                                                                           >  1:132505/1‑124 (MQ=255)                                                        gagaAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTAc                                                                                           <  2:132505/124‑1 (MQ=255)                                                             aCCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAg                                                                      <  1:305573/140‑1 (MQ=255)                                                             aCCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAg                                                                      <  1:128486/140‑1 (MQ=255)                                                             aCCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAg                                                                      <  1:226458/140‑1 (MQ=255)                                                                              tCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATc                                                     >  1:273064/1‑140 (MQ=255)                                                                                     aCCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGCGCGGCATCGGAAAAt                                              <  1:671386/140‑1 (MQ=255)                                                                                                    cagGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACg                               >  2:180156/1‑140 (MQ=255)                                                                                                       gATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCtt                            >  1:324500/1‑140 (MQ=255)                                                                                                        aTCGGGTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCttt                           >  1:766047/1‑140 (MQ=255)                                                                                                           gggTTTTCAGCAATCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGc                        <  1:482698/140‑1 (MQ=255)                                                                                                                       aTCTGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCt            <  2:324500/140‑1 (MQ=255)                                                                                                                          tGGCGTTGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTg         >  1:16120/1‑140 (MQ=255)                                                                                                                               ttGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCtt           >  1:56382/1‑133 (MQ=255)                                                                                                                               ttGGATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCtt           <  2:56382/133‑1 (MQ=255)                                                                                                                                 ggATTTTTCCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTAtt  >  1:153917/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    ATCAACATAGGCAAAACGTTCGCCACATGCGGCAAGCGCCTGGACTGCCTGGGCAGAGAAACCGCAGCTCGGCAGTTTCGGTGAACCTTTCATGTACAGCAGGATCGGGTTTTCAGCAATCTGGCGTTGGATTTTTTCGATAGTGGTGCTCATTGTCTTGCTTCCTCAAACTTCTTTACGGCAGTAATCTGACATTGTAGCGGGTCAGTGCGGCATCGGAAAATAACATTTTCATCACGCTTTTGCTATTTTATCCCTTTGCTCTATT  >  NC_000913/1733949‑1734216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑