Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,739,481	A→G	R106R (CGT→CGC)	ydhB ←	putative LysR family DNA‑binding transcriptional regulator YdhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,739,481	0	A	G	100.0%	127.8 / NA	35	R106R (CGT→CGC)	ydhB	putative LysR family DNA‑binding transcriptional regulator YdhB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/20);  total (15/20)

CGCGCGTGTTGCGCCAATCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCC  >  NC_000913/1739346‑1739615                                                                                                                                        |                                                                                                                                       cgcgcgTGTTGCGCCAATCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGtt                                                                                                                                    <  2:535608/140‑1 (MQ=255)   cgcgTGTTGCGCCAATCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCt                                                                                                                                  >  2:424087/1‑140 (MQ=255)     cgTGTTGCGCCAATCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTgg                                                                                                                                <  1:173912/140‑1 (MQ=255)     cgTGTTGCGCCAATCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTgg                                                                                                                                <  2:560596/140‑1 (MQ=255)          tGCGCCAATCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGa                                                                                                                           <  2:479314/140‑1 (MQ=255)                 aTCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAAtatt                                                                                                                    <  2:263458/140‑1 (MQ=255)                  tCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAAtatta                                                                                                                   <  2:326754/140‑1 (MQ=255)                          tCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCa                                                                                                               <  2:82757/136‑1 (MQ=255)                          tCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCa                                                                                                               >  1:82757/1‑136 (MQ=255)                           ccACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCg                                                                                                          <  2:189988/140‑1 (MQ=255)                                                         cacCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCt                                                                            <  2:797628/140‑1 (MQ=255)                                                           ccGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTAt                                                                          <  1:73591/140‑1 (MQ=255)                                                                    acTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACa                                                                 <  2:340726/140‑1 (MQ=255)                                                                           gAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTgg                                                          >  1:813539/1‑140 (MQ=255)                                                                               gACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCggg                                                      <  1:424087/140‑1 (MQ=255)                                                                                aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCgggg                                                     >  1:771235/1‑139 (MQ=255)                                                                                aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGt                                                     >  1:536404/1‑140 (MQ=255)                                                                                aCAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGt                                                     >  1:327686/1‑140 (MQ=255)                                                                                         tCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAt                                            >  1:685819/1‑140 (MQ=255)                                                                                          cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                           <  1:445491/140‑1 (MQ=255)                                                                                          cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                           >  1:668017/1‑140 (MQ=255)                                                                                          cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                           >  1:396672/1‑140 (MQ=255)                                                                                          cGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCAtt                                           >  1:284139/1‑140 (MQ=255)                                                                                                   caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGaca                                  <  2:327686/140‑1 (MQ=255)                                                                                                   caAAATGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGaca                                  <  2:685819/140‑1 (MQ=255)                                                                                                      aaTGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGacaaca                               <  2:618017/140‑1 (MQ=255)                                                                                                        tGGCGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACATTATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACaga                             <  1:309888/140‑1 (MQ=255)                                                                                                           cGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAgcg                          >  2:428666/1‑140 (MQ=255)                                                                                                           cGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAgcg                          >  2:37799/1‑140 (MQ=255)                                                                                                           cGATAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAgcg                          >  2:283325/1‑140 (MQ=255)                                                                                                             aTAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTTTCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGcc                        <  2:466523/140‑1 (MQ=255)                                                                                                             aTAAAAATCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGcc                        >  1:112576/1‑140 (MQ=255)                                                                                                                aaaaTCAACGATCATCTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCctt                     <  1:283325/140‑1 (MQ=255)                                                                                                                             atcTGCCGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCa        <  2:536404/140‑1 (MQ=255)                                                                                                                                  ccGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTc   <  1:428666/140‑1 (MQ=255)                                                                                                                                   cGTGTGCGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTcc  >  1:275522/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CGCGCGTGTTGCGCCAATCGCCAGTTCCACGCGCCCGTCGGAAAGCGCATCCCAGACACCGTTGAACACTTCCTGAAAGACAAGAAGTTCGACATCATCAAAATGGCGATAAAAATCAACGATCATCTGCCGTGTACGTTCTGGCCTGACAATATTATCCACTGCGATAGCTAACTGACCGCGCCAGCCGTTCGCTATCTGCTGACATTGCTGGCGGGTGATCTGCATTTTTTTGACAACAGAGCGCCCTTCTTTGAGAAACCACGCTCC  >  NC_000913/1739346‑1739615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑