Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,980	A→G	A342A (GCA→GCG)	yeaH →	DUF444 domain‑containing protein YeaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,980	0	A	G	100.0%	183.6 / NA	49	A342A (GCA→GCG)	yeaH	DUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

GCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGC  >  NC_000913/1869846‑1870113                                                                                                                                       |                                                                                                                                      gCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGc                                                                                                                                  >  1:184105/1‑140 (MQ=255) gCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGc                                                                                                                                  >  2:350624/1‑140 (MQ=255)  cGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCa                                                                                                                                 <  1:565283/140‑1 (MQ=255)     aagaagTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCg                                                                                                                              >  2:470411/1‑140 (MQ=255)      agaagTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCgg                                                                                                                             <  2:356557/140‑1 (MQ=255)           tCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGc                                                                                                                        >  1:62076/1‑140 (MQ=255)           tCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGc                                                                                                                        >  1:406939/1‑140 (MQ=255)                  aCATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACt                                                                                                                 <  1:90985/140‑1 (MQ=255)                     tGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTggg                                                                                                              >  2:474327/1‑140 (MQ=255)                         tttttCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGa                                                                                                          <  2:62076/140‑1 (MQ=255)                          ttttCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGAt                                                                                                         <  2:392469/140‑1 (MQ=255)                              cTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGAct                                                                                                     <  1:746057/140‑1 (MQ=255)                                        gAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTg                                                                                           <  1:470411/140‑1 (MQ=255)                                           aCAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCa                                                                                        <  2:496208/140‑1 (MQ=255)                                                  gcACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATc                                                                                 >  2:399386/1‑140 (MQ=255)                                                                 gcgcCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAatta                                                                  >  2:717708/1‑140 (MQ=255)                                                                   gcCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAattatt                                                                >  1:298922/1‑140 (MQ=255)                                                                     ccTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTAc                                                              <  2:184105/140‑1 (MQ=255)                                                                      cTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTAcc                                                             <  1:417784/140‑1 (MQ=255)                                                                       tGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCt                                                            >  1:78410/1‑140 (MQ=255)                                                                           aCTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCtgttg                                                        <  2:490606/140‑1 (MQ=255)                                                                           aCTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCtgttg                                                        <  2:638443/140‑1 (MQ=255)                                                                             tGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTgttgtt                                                      <  2:786422/140‑1 (MQ=255)                                                                              gatggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTc                                                     <  1:350624/140‑1 (MQ=255)                                                                               atggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCg                                                    >  1:164107/1‑140 (MQ=255)                                                                                tggatgAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGt                                                   <  1:474327/140‑1 (MQ=255)                                                                                       ggTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACa                                            >  2:550804/1‑140 (MQ=255)                                                                                             gAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCtata                                      >  2:742543/1‑140 (MQ=255)                                                                                              aaaGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTatat                                     >  1:910123/1‑140 (MQ=255)                                                                                                  agCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAg                                           <  2:316485/130‑1 (MQ=255)                                                                                                  agCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAg                                           >  1:316485/1‑130 (MQ=255)                                                                                                  agCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGaa                                 >  2:771575/1‑140 (MQ=255)                                                                                                  agCGTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGaa                                 >  2:761932/1‑140 (MQ=255)                                                                                                     gTTATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAAtt                              >  1:756219/1‑140 (MQ=255)                                                                                                      ttATAACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTa                             >  1:472489/1‑140 (MQ=255)                                                                                                       tataACCCGGCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTAc                            <  1:399386/140‑1 (MQ=255)                                                                                                               ggCACAGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTg                    <  1:390116/140‑1 (MQ=255)                                                                                                                 cacaGTGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGca                  >  2:384404/1‑140 (MQ=255)                                                                                                                      tGGAATATTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCa             >  1:400366/1‑140 (MQ=255)                                                                                                                           tatTTACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACAt        >  2:73030/1‑140 (MQ=255)                                                                                                                               tACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATtgtg    <  2:406939/140‑1 (MQ=255)                                                                                                                               tACGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATtgtg    <  2:241150/140‑1 (MQ=255)                                                                                                                                aCGCTGCGCAAGCNTCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTgg   >  1:740332/1‑140 (MQ=255)                                                                                                                                aCGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTgg   >  1:257551/1‑140 (MQ=255)                                                                                                                                aCGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTgg   <  2:756219/140‑1 (MQ=255)                                                                                                                                aCGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTgg   >  1:397392/1‑140 (MQ=255)                                                                                                                                 cGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGc  <  1:73030/140‑1 (MQ=255)                                                                                                                                 cGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGc  <  1:384404/140‑1 (MQ=255)                                                                                                                                 cGCTGCGCAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGc  <  1:473259/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GCGAAAGAAGTCGATGAACATGAGTTTTTCTACTCGCAGGAAACAGGCGGCACCATTGTTTCCAGCGCCCTGAAACTGATGGATGAGGTAGTGAAAGAGCGTTATAACCCGGCACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGC  >  NC_000913/1869846‑1870113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑