| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,933,732 | G→A | I291I (ATC→ATT) | edd ← | phosphogluconate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,933,732 | 0 | G | A | 98.1% | 185.9 / ‑3.5 | 53 | coding (873/1812 nt) | edd | phosphogluconate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (27/25); minor base . (1/0); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACGATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAGCGGAGAATCCGGATGAA > NC_000913/1933604‑1933865 | gCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGtt > 1:801348/1‑137 (MQ=255)gCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGtt < 2:801348/137‑1 (MQ=255) ccATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTcacc > 1:485961/1‑140 (MQ=255) aTCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTcaccac < 1:493085/140‑1 (MQ=255) tCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTcaccacc > 1:493888/1‑140 (MQ=255) acaTCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATc < 1:170392/140‑1 (MQ=255) acaTCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATc < 2:493888/140‑1 (MQ=255) aTCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGa < 2:367521/140‑1 (MQ=255) tCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGat < 1:743960/140‑1 (MQ=255) cAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGatc < 2:890186/140‑1 (MQ=255) gTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTAc > 1:361336/1‑140 (MQ=255) gTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAAC‑AAACCGTTCACCACCACTTTCTCATCGATCATCTTAcc > 1:96490/1‑140 (MQ=255) tCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTAcc > 2:809900/1‑140 (MQ=255) gaAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCg < 1:110405/140‑1 (MQ=255) aGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGc < 2:126909/140‑1 (MQ=255) tCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCAt < 1:480779/140‑1 (MQ=255) aGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCAGCATCcattca < 2:911773/140‑1 (MQ=255) gTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCcattcat < 1:835958/140‑1 (MQ=255) ttAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCcattcatt > 2:782440/1‑140 (MQ=255) tAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTcatta > 1:91982/1‑140 (MQ=255) cTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTcattaccat < 1:906092/140‑1 (MQ=255) cGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTc < 2:91982/140‑1 (MQ=255) ggCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCa < 1:809900/140‑1 (MQ=255) ggCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCa > 2:792214/1‑140 (MQ=255) gCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCAt > 1:268393/1‑140 (MQ=255) gCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCAt > 2:52308/1‑140 (MQ=255) cgcgcgcgCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGc < 2:632147/140‑1 (MQ=255) gcgcgcgcCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCg > 2:773298/1‑140 (MQ=255) gcgcgcgcCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCg > 1:484784/1‑140 (MQ=255) cgcgcgcCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTACAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCgt > 2:637442/1‑140 (MQ=255) aTCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTg < 2:268393/140‑1 (MQ=255) gCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCTTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGAcg > 2:637989/1‑140 (MQ=255) gCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGAcg < 1:792214/140‑1 (MQ=255) gCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTgcg < 1:160031/140‑1 (MQ=255) gtgtGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGt > 2:517297/1‑140 (MQ=255) gtgtGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGt < 1:922071/140‑1 (MQ=255) tgGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCaa > 1:33469/1‑140 (MQ=255) tgGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCaa > 1:431204/1‑140 (MQ=255) tgGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCaa > 1:671390/1‑140 (MQ=255) tGGAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATcg > 2:376572/1‑140 (MQ=255) ggAACCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATcgc > 2:761794/1‑140 (MQ=255) aaCCACCGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCgcgc > 2:330135/1‑140 (MQ=255) accaccGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCa < 2:484784/140‑1 (MQ=255) ccaccGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAg > 2:607120/1‑140 (MQ=255) ccaccGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAg > 2:102574/1‑140 (MQ=255) ccaccGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAg > 1:3873/1‑140 (MQ=255) ccaccGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATAgcgc < 1:383855/138‑1 (MQ=255) ccaccGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATAgcgc > 2:383855/1‑138 (MQ=255) cGGTCGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAGCGga < 1:773298/140‑1 (MQ=255) cGCCAGCAGTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAGCGGAGAAt > 1:781566/1‑140 (MQ=255) gcagTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAGCGGAGAATCCGGa < 2:431204/140‑1 (MQ=255) agTGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAGCGGAGAATCCGGATg < 2:361336/140‑1 (MQ=255) tGCAACAATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAGCGGAGAATCCGGATGaa > 1:8014/1‑140 (MQ=255) | GCCATCAGCGGTACAACATCAGAAAGGTCAGAGAAGTCATCCCAGTTAATCTGAATACCGGCCGCGCGCGCCATCGCCACCAGGTGCATGGTGTGGTTAGTGGAACCACCGGTCGCCAGCAGTGCAACGATACCGTTCACCACCACTTTCTCATCGATCATCTTACCGATCGGCATCCATTCATTACCATTACCGGTCATGCGTGTAACCTGACGCGCAGCTGCGGCGGTCAAAGCATCGCGCAGCGGAGAATCCGGATGAA > NC_000913/1933604‑1933865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |