Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,229,119	C→T	intergenic (+54/+319)	yohP → / ← dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,229,119	0	C	T	100.0%	89.0 / NA	26	intergenic (+54/+319)	yohP/dusC	putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/16);  total (10/16)

AAATTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCT  >  NC_000913/2228986‑2229255                                                                                                                                      |                                                                                                                                         aaaTTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTg                                                                                                                                    <  1:42151/140‑1 (MQ=255)      ataCTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTc                                                                                                                               <  1:80784/140‑1 (MQ=255)            tGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCa                                                                                                                         >  1:747644/1‑140 (MQ=255)             gggCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCAt                                                                                                                        <  2:17352/140‑1 (MQ=255)             gggCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCAt                                                                                                                        <  1:865247/140‑1 (MQ=255)              ggCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCAtg                                                                                                                       >  2:332576/1‑140 (MQ=255)              ggCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCAtg                                                                                                                       >  1:811031/1‑140 (MQ=255)                  gTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCa                                                                                                                   <  2:397105/140‑1 (MQ=255)                    aTTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCAtt                                                                                                                 <  2:901595/140‑1 (MQ=255)                      tGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGc                                                                                                               <  1:332576/140‑1 (MQ=255)                      tGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGc                                                                                                               <  1:785914/140‑1 (MQ=255)                        attattTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCtt                                                                                                             >  1:92367/1‑140 (MQ=255)                             tttCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGc                                                                                                               >  1:723407/1‑133 (MQ=255)                             tttCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGc                                                                                                               <  2:723407/133‑1 (MQ=255)                             tttCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCatat                                                                                                        >  1:340865/1‑140 (MQ=255)                                  tGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCa                                                                                                   <  1:508843/140‑1 (MQ=255)                                                             tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                        <  2:340865/140‑1 (MQ=255)                                                                  aaGATGATATTTTAAAATCAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCCTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAt                                                                   >  2:41613/1‑140 (MQ=255)                                                                         tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgcc                                                            <  2:747644/140‑1 (MQ=255)                                                                                     aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCAcc                                                >  1:531332/1‑140 (MQ=255)                                                                                               atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGctct                                      >  1:243065/1‑140 (MQ=255)                                                                                                                cGAGAATATTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgccg                     <  2:266640/140‑1 (MQ=255)                                                                                                                      tatTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgcgc               <  2:721169/140‑1 (MQ=255)                                                                                                                       atTTCAGTCTCTCATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg              <  2:811031/140‑1 (MQ=255)                                                                                                                                tctcATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCCTGCACCCCCCGGCTCTGGCGAACAAAAACGCCGCTGCGCGGTGCCCTCg     >  1:236797/1‑140 (MQ=255)                                                                                                                                   cATTCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt  <  2:531332/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         AAATTATACTCTGGGCTGTATTGATTATTTTCCTGATTGGGCTACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCT  >  NC_000913/2228986‑2229255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑