Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,541,754	G→A	L253L (CTC→CTT)	cysU ←	sulfate/thiosulfate ABC transporter inner membrane subunit CysU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,541,754	0	G	A	100.0%	106.9 / NA	31	L253L (CTC→CTT)	cysU	sulfate/thiosulfate ABC transporter inner membrane subunit CysU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/10);  total (21/10)

ACCATTTGCCCCAGTTAATCGGGCGCGCGTCATAACGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAG  >  NC_000913/2541619‑2541888                                                                                                                                        |                                                                                                                                        aCCATTTGCCCCAGTTAATCGGGCGCGCGTCATAACGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGa                                                                                                                                     >  2:491551/1‑140 (MQ=255)                    ctggCGCGCGTCATAACGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGcgct                                                                                                                  <  2:625056/138‑1 (MQ=255)                    cGGGCGCGCGTCATAACGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGcgct                                                                                                                  <  2:69328/140‑1 (MQ=255)                        cgcgcgTCATAACGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGcgctcgct                                                                                                              >  1:435547/1‑140 (MQ=255)                            cgTCATAACGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCg                                                                                                          <  1:559635/140‑1 (MQ=255)                                  aaCGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAAt                                                                                                    <  2:596005/140‑1 (MQ=255)                                     gCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCaa                                                                                                 <  2:435547/140‑1 (MQ=255)                                       ttCAATTGGGTAACTTCCGCCATTAATGACCTAGCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAAc                                                                                               >  2:749427/1‑140 (MQ=255)                                       ttCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAAc                                                                                               >  2:279278/1‑140 (MQ=255)                                         cAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTc                                                                                             >  1:257235/1‑140 (MQ=255)                                                gTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAg                                                                                      >  1:309665/1‑140 (MQ=255)                                                       ccGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑aa                                                                              >  1:368074/1‑140 (MQ=255)                                                          ccATTAATGACCTACCACATGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑aaaaa                                                                           >  1:802366/1‑140 (MQ=255)                                                                        ccACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTc                                                             >  1:441149/1‑140 (MQ=255)                                                                             cGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCa                                                            >  2:732358/1‑136 (MQ=255)                                                                             cGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCa                                                            <  1:732358/136‑1 (MQ=255)                                                                             cGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTc                                                        >  2:44332/1‑140 (MQ=255)                                                                             cGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTc                                                        >  2:113770/1‑140 (MQ=255)                                                                              gCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTcc                                                       >  1:492516/1‑140 (MQ=255)                                                                                  aCCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCt                                                   >  1:162271/1‑140 (MQ=255)                                                                                      aaGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCa                                               <  2:257235/140‑1 (MQ=255)                                                                                                        ttAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGAt                             >  1:743165/1‑140 (MQ=255)                                                                                                         tAATTGAGAACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATa                            >  2:625502/1‑140 (MQ=255)                                                                                                                gaACAGCAGCAGCAGAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCa                     >  1:883713/1‑140 (MQ=255)                                                                                                                      cagcagcagAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCgcgc               >  1:95889/1‑140 (MQ=255)                                                                                                                      cagcagcagAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCgcgc               >  1:50203/1‑140 (MQ=255)                                                                                                                           gcagAGATGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAAt          >  2:361392/1‑140 (MQ=255)                                                                                                                                gaTGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCAcc     <  1:749427/140‑1 (MQ=255)                                                                                                                                gaTGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCAcc     <  1:113770/140‑1 (MQ=255)                                                                                                                                 aTGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCa    <  1:491551/140‑1 (MQ=255)                                                                                                                                    ccGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAg  >  2:58357/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        ACCATTTGCCCCAGTTAATCGGGCGCGCGTCATAACGCTTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCAC‑AAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAG  >  NC_000913/2541619‑2541888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑