Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,892,308	G→A	G32E (GGG→GAG)	queD →	6‑carboxy‑5,6,7,8‑tetrahydropterin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,892,308	0	G	A	100.0%	198.9 / NA	56	G32E (GGG→GAG)	queD	6‑carboxy‑5,6,7,8‑tetrahydropterin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/31);  total (25/31)

TTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGAT  >  NC_000913/2892172‑2892441                                                                                                                                         |                                                                                                                                      ttCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCa                                                                                                                                    <  2:62063/140‑1 (MQ=255) ttCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCa                                                                                                                                    <  1:366029/140‑1 (MQ=255)  tCCGGTACTCTACGGCGGTTTTTTCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCAt                                                                                                                                   >  2:729476/1‑140 (MQ=255)        aCTCTACGGCGGTTTTTTTGTCCTTTAGAGAAAATATGATGTCCACCACGTTATTTAAAAATTTCACCTTCCAAACCCCTCACCGCTTTCCACACGTCCCCGGAGGGCATAAAATTGGGCGCCCGCACGAGCATTCCttt                                                                                                                             >  2:381990/1‑140 (MQ=255)              cggcggTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCCGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTg                                                                                                                       <  2:634852/140‑1 (MQ=255)               ggcggTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGc                                                                                                                      <  2:871118/140‑1 (MQ=255)                 cggTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGa                                                                                                                    <  1:486461/140‑1 (MQ=255)                        aTCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGaaa                                                                                                             >  1:82838/1‑140 (MQ=255)                               tGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCgg                                                                                                      <  1:564224/140‑1 (MQ=255)                                gTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCggg                                                                                                     >  2:531325/1‑140 (MQ=255)                                      aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  1:273901/1‑140 (MQ=255)                                      aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  1:514369/1‑140 (MQ=255)                                      aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  2:166549/1‑140 (MQ=255)                                      aaaTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTc                                                                                               >  2:494653/1‑140 (MQ=255)                                        aTTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGa                                                                                             >  1:2046/1‑140 (MQ=255)                                           atgatgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATcc                                                                                          >  1:515644/1‑140 (MQ=255)                                           atgatgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATcc                                                                                          >  1:542649/1‑140 (MQ=255)                                              atgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCa                                                                                       <  1:381990/140‑1 (MQ=255)                                               tgTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCAt                                                                                      <  2:554382/140‑1 (MQ=255)                                                gTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATc                                                                                           <  1:536262/134‑1 (MQ=255)                                                gTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATc                                                                                           >  2:536262/1‑134 (MQ=255)                                                 tCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATAc                                                                                    >  1:186338/1‑140 (MQ=255)                                                  ccaccaCGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACg                                                                                   <  1:919532/140‑1 (MQ=255)                                                   caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACgg                                                                                  >  2:487416/1‑140 (MQ=255)                                                   caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACgg                                                                                  >  1:542640/1‑140 (MQ=255)                                                   caccacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACgg                                                                                  >  2:303701/1‑140 (MQ=255)                                                      cacGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCt                                                                               <  2:514369/140‑1 (MQ=255)                                                         gTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGa                                                                            <  1:565962/140‑1 (MQ=255)                                                           tATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGAtt                                                                          >  1:864924/1‑140 (MQ=255)                                                             tttAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTAt                                                                        >  2:303225/1‑140 (MQ=255)                                                                     tttCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCg                                                                <  2:551577/140‑1 (MQ=255)                                                                      ttCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGc                                                               >  1:823485/1‑140 (MQ=255)                                                                       tCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCt                                                              <  1:648634/140‑1 (MQ=255)                                                                       tCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCt                                                              <  2:82838/140‑1 (MQ=255)                                                                           cTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAAc                                                          <  1:143033/140‑1 (MQ=255)                                                                                  gCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgc                                                   <  1:555775/140‑1 (MQ=255)                                                                                     gCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAgcggc                                                >  1:207103/1‑140 (MQ=255)                                                                                         aCCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGttt                                            <  1:318743/140‑1 (MQ=255)                                                                                           cGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATAATCGATTTCGCTGAACTAAAAGCGGCGTTTaa                                          <  2:355566/140‑1 (MQ=255)                                                                                              ttACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAaacc                                       <  1:367626/140‑1 (MQ=255)                                                                                                        tCCCGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAg                             >  2:569984/1‑140 (MQ=255)                                                                                                         cccGGAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAgc                            <  2:864924/140‑1 (MQ=255)                                                                                                            ggAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGcc                         <  2:823485/140‑1 (MQ=255)                                                                                                             gAAGGGCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCt                        <  2:186338/140‑1 (MQ=255)                                                                                                                gggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGa                     <  1:729476/140‑1 (MQ=255)                                                                                                                gggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGa                     <  1:569984/140‑1 (MQ=255)                                                                                                                gggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGa                     <  2:2046/140‑1 (MQ=255)                                                                                                                 ggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGAt                    <  2:515644/140‑1 (MQ=255)                                                                                                                 ggCATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGAt                    >  1:518668/1‑140 (MQ=255)                                                                                                                   cATAAATGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCa                  >  1:745713/1‑140 (MQ=255)                                                                                                                      aaaTGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCGCCa               <  1:494653/140‑1 (MQ=255)                                                                                                                        aTGTGGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCAtt             <  1:303701/140‑1 (MQ=255)                                                                                                                           tgGTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTCTAAACCAACCTACGAGCGCCTCGATCACCATTAtc          >  2:752356/1‑140 (MQ=255)                                                                                                                            ggTCGCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTAtct         <  2:542640/140‑1 (MQ=255)                                                                                                                                gCCTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAAt     >  2:100837/1‑140 (MQ=255)                                                                                                                                  cTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGa   <  1:487416/140‑1 (MQ=255)                                                                                                                                  cTGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGa   >  1:145423/1‑140 (MQ=255)                                                                                                                                   tGCACGAGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGat  >  1:677816/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TTCCGGTACTCTACGGCGGTTTTATCGTCCTGTAGAGAAATTATGATGTCCACCACGTTATTTAAAGATTTCACCTTCGAAGCCGCTCACCGCTTACCACACGTCCCGGAAGGGCATAAATGTGGTCGCCTGCACGGGCATTCCTTTATGGTGCGACTGGAAATTACCGGGGAAGTCGATCCGCATACGGGCTGGATTATCGATTTCGCTGAACTAAAAGCGGCGTTTAAACCAACCTACGAGCGCCTCGATCACCATTATCTCAATGAT  >  NC_000913/2892172‑2892441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑