Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,967,846	C→T	V197M (GTG→ATG)	ptsP ←	phosphoenolpyruvate‑protein phosphotransferase PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,967,846	0	C	T	100.0%	158.8 / NA	46	V197M (GTG→ATG)	ptsP	phosphoenolpyruvate‑protein phosphotransferase PtsP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/25);  total (21/25)

AAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGC  >  NC_000913/2967710‑2967981                                                                                                                                         |                                                                                                                                        aaaaTAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTg                                                                                                                                      >  2:360755/1‑140 (MQ=255)  aaaTAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGt                                                                                                                                     >  1:607190/1‑140 (MQ=255)   aaTAGCCGCCATTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGtt                                                                                                                                    <  2:796556/140‑1 (MQ=255)       gccgccGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCAt                                                                                                                                >  2:360230/1‑140 (MQ=255)       gccgccGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCAt                                                                                                                                >  1:530694/1‑140 (MQ=255)        ccgccgTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCAtt                                                                                                                               >  2:276955/1‑140 (MQ=255)              tttCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGt                                                                                                                         <  2:835514/140‑1 (MQ=255)                       gtgCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCa                                                                                                                <  1:536272/140‑1 (MQ=255)                        tgCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCAt                                                                                                               >  1:340322/1‑140 (MQ=255)                            ccGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTg                                                                                                           >  1:53741/1‑140 (MQ=255)                                  gcAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGcc                                                                                                     >  2:521956/1‑140 (MQ=255)                                  gcAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGcc                                                                                                     >  1:464740/1‑140 (MQ=255)                                      aGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCg                                                                                                 <  2:209390/140‑1 (MQ=255)                                         gTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCa                                                                                              <  1:360755/140‑1 (MQ=255)                                            tGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATTCATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATc                                                                                           >  2:184687/1‑140 (MQ=255)                                            tGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATc                                                                                           <  1:521956/140‑1 (MQ=255)                                                 tAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCcac                                                                                      >  2:407062/1‑140 (MQ=255)                                                  aGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCcaca                                                                                     <  2:676500/140‑1 (MQ=255)                                                        gAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGt                                                                               <  1:582872/140‑1 (MQ=255)                                                        gAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGt                                                                               >  2:545018/1‑140 (MQ=255)                                                          aaCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGtgc                                                                             <  2:340322/140‑1 (MQ=255)                                                          aaCTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGtgc                                                                             <  2:607190/140‑1 (MQ=255)                                                                     cGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATg                                                                  >  1:350643/1‑140 (MQ=255)                                                                       cttcttCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATgcg                                                                <  2:860429/140‑1 (MQ=255)                                                                                gcgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCTGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcgc                                                       >  2:852216/1‑140 (MQ=255)                                                                                gcgcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTcgc                                                       <  2:350643/140‑1 (MQ=255)                                                                                  gcCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGt                                                     >  1:371008/1‑140 (MQ=255)                                                                                          cAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGat                                             <  1:812265/140‑1 (MQ=255)                                                                                          cAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGat                                             <  1:779359/140‑1 (MQ=255)                                                                                           aGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGata                                            <  2:332993/140‑1 (MQ=255)                                                                                             tCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATAtt                                          >  2:830809/1‑140 (MQ=255)                                                                                               gTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGc                                        <  1:289248/140‑1 (MQ=255)                                                                                                  cgcgTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACATTTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCa                                     >  2:44531/1‑140 (MQ=255)                                                                                                      ttCCAGAGCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACa                                 <  1:276955/140‑1 (MQ=255)                                                                                                            agCCGGATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAg                           <  2:371008/140‑1 (MQ=255)                                                                                                                 gATCCAGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAAc                      >  1:783102/1‑140 (MQ=255)                                                                                                                      aGCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGTTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGa                 <  1:360230/140‑1 (MQ=255)                                                                                                                       gCGTTGATGCCTGATACATTTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGAc                <  1:44531/140‑1 (MQ=255)                                                                                                                       gCGTTGATGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGAc                <  2:464740/140‑1 (MQ=255)                                                                                                                             aTGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGTGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaa          >  1:124007/1‑140 (MQ=255)                                                                                                                             aTGCCTGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTGTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGagaa          >  2:860892/1‑140 (MQ=255)                                                                                                                                  tGATACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAAt     >  2:254869/1‑140 (MQ=255)                                                                                                                                    aTACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAg   <  1:741554/140‑1 (MQ=255)                                                                                                                                    aTACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAg   <  1:545018/140‑1 (MQ=255)                                                                                                                                    aTACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAg   <  1:54042/140‑1 (MQ=255)                                                                                                                                     tACATCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGc  <  2:783102/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AAAATAGCCGCCGTTTCTTTTTGTGCACCGGCGGCAAAGCGTTTGCTGTAGCGGCGAAACTCGTTTGCCGCTTCTTCCAGCGCCCCGGTCAGTCGTTCGCGTTCCAGAGCCGGATCCAGCGTTGATGCCTGATACACCTGTTCCATTAAAGGTAACGTGGCATCCTGCCAGCCTTCGGCAATCGCCACACCAGGTGCTGCCGGTAATGCGCGGATTCGCGTCTGGCGATATTGCCCAAACAAGGCAGTCAACTGCGACTGAGAAAGAATAGC  >  NC_000913/2967710‑2967981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑