Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,017,609	A→G	D517G (GAC→GGC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,017,609	0	A	G	100.0%	130.8 / NA	36	D517G (GAC→GGC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/9);  total (27/9)

ATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGC  >  NC_000913/3017479‑3017741                                                                                                                                   |                                                                                                                                     aTCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGAGCGCt                                                                                                                             >  2:816044/1‑140 (MQ=255)        tGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATc                                                                                                                      >  1:478569/1‑140 (MQ=255)                  acacCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGa                                                                                                            >  1:168869/1‑140 (MQ=255)                       gCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGaaaaaa                                                                                                       >  2:687253/1‑140 (MQ=255)                                             cATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTggg                                                                                 >  2:775183/1‑140 (MQ=255)                                              aTCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGa                                                                                >  2:482320/1‑140 (MQ=255)                                                  aCCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGaa                                                                            >  1:677623/1‑140 (MQ=255)                                                   ccAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAat                                                                           >  2:108110/1‑140 (MQ=255)                                                    caacgcaacgCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAata                                                                          >  2:786157/1‑140 (MQ=255)                                                      acgcaacgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAAtata                                                                        >  1:496216/1‑140 (MQ=255)                                                        gcaacgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAg                                                                      >  1:45296/1‑140 (MQ=255)                                                        gcaacgcTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAg                                                                      >  2:222220/1‑140 (MQ=255)                                                                 tGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTgg                                                             >  1:707669/1‑140 (MQ=255)                                                                     cGCCATTCCCGGTAATATTTGCGACCCCCAGTCCCAATACACCTGTCCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGcaca                                                         <  2:316752/140‑1 (MQ=255)                                                                             ccGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGcc                                                 >  1:262514/1‑140 (MQ=255)                                                                                gTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGt                                              >  1:658682/1‑140 (MQ=255)                                                                                 taatttttGCGATCACCAGTGCAAATACAACTGTCCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGtt                                             <  1:52048/135‑1 (MQ=255)                                                                                 aattttttGCGACCACCAGTGCAAACACAACTGTCCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGtt                                             <  2:278134/135‑1 (MQ=255)                                                                                  cATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTc                                            >  2:331748/1‑140 (MQ=255)                                                                                  cATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTc                                            >  1:722854/1‑140 (MQ=255)                                                                                     atTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTgg                                         >  1:347466/1‑140 (MQ=255)                                                                                         gCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCa                                     <  1:331748/140‑1 (MQ=255)                                                                                           gATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACg                                   >  1:717492/1‑140 (MQ=255)                                                                                           gATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACg                                   >  2:461276/1‑140 (MQ=255)                                                                                               aCCAGTGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGcctg                               >  1:278134/1‑138 (MQ=255)                                                                                                    tGCCAATACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGt                          >  1:180053/1‑140 (MQ=255)                                                                                                        aaTACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGcc                      <  1:222220/140‑1 (MQ=255)                                                                                                         aTACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCg                     >  1:864701/1‑140 (MQ=255)                                                                                                          tACAACTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGt                    >  1:391843/1‑140 (MQ=255)                                                                                                             aaCTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAt                 <  1:60765/140‑1 (MQ=255)                                                                                                             aaCTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAt                 <  2:707669/140‑1 (MQ=255)                                                                                                              aCTGTACCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGAtt                <  2:347466/140‑1 (MQ=255)                                                                                                                   aCCCGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGc           <  2:478569/140‑1 (MQ=255)                                                                                                                      cGCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAgg        >  2:788407/1‑140 (MQ=255)                                                                                                                       gCCTGGATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGt       >  2:102732/1‑140 (MQ=255)                                                                                                                            gATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCcggc  <  1:566204/140‑1 (MQ=255)                                                                                                                            gATTACGGCAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCcggc  >  2:308998/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                     ATCCGTCTGCTTGGCGAACACCGCTATGCCGACGCGCTGGAACTCATCTACCAACGCAACGCTCTGCCCGCCATTACCGGTCATATTTGCGATCACCAGTGCCAATACAACTGTACCCGCCTGGATTACGACAGTGCGCTGAATATCCGCGAACTGAAAAAAGTCGCGCTGGAAAAAGGTTGGGATGAATATAAGCAACGCTGGCACAAACCAGCCGGTTCTGGTTCACGCCATCCGGTTGCCGTGATTGGTGCAGGTCCGGC  >  NC_000913/3017479‑3017741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑