Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,101,968	C→T	A56T (GCC→ACC)	yggL ←	protein YggL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,101,968	0	C	T	100.0%	138.6 / NA	41	A56T (GCC→ACC)	yggL	protein YggL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

TGCGTACCTCATCCAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGAATTCGTCGATG  >  NC_000913/3101839‑3102092                                                                                                                                  |                                                                                                                             tGCGTACCTCATCCAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTgttc                                                                                                                    <  1:69812/140‑1 (MQ=255)   cGTACCTCATCCAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTgttcgg                                                                                                                  <  1:324249/140‑1 (MQ=255)   cGTACCTCATCCAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTgttcgg                                                                                                                  <  2:618131/140‑1 (MQ=255)         tCATCCAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGAt                                                                                                            <  2:891774/140‑1 (MQ=255)           aTCCAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATaa                                                                                                          >  1:696198/1‑140 (MQ=255)             ccAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATa                                                                                                           <  2:280918/137‑1 (MQ=255)             ccAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATa                                                                                                           >  1:280918/1‑137 (MQ=255)             ccAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAAcc                                                                                                        >  1:635489/1‑140 (MQ=255)                gTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCg                                                                                                     <  2:679342/140‑1 (MQ=255)                gTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCg                                                                                                     >  2:560542/1‑140 (MQ=255)                gTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCACACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCg                                                                                                     <  2:824706/140‑1 (MQ=255)                  ttGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGtt                                                                                                   >  1:257519/1‑140 (MQ=255)                   tGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTta                                                                                                  <  2:503976/140‑1 (MQ=255)                         ctTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATaaaa                                                                                            >  1:547117/1‑140 (MQ=255)                         ctTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATaaaa                                                                                            >  1:339097/1‑140 (MQ=255)                              aGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAatcatc                                                                                       <  2:547117/140‑1 (MQ=255)                              aGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAatcatc                                                                                       <  2:339097/140‑1 (MQ=255)                                ccACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCaa                                                                                     <  2:578566/140‑1 (MQ=255)                                   cTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACgg                                                                                  <  1:109549/140‑1 (MQ=255)                                    ttACGCACAATCGTCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGt                                                                                 <  1:265771/140‑1 (MQ=255)                                    ttACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGt                                                                                 >  2:790299/1‑140 (MQ=255)                                    ttACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGt                                                                                 >  1:521632/1‑140 (MQ=255)                                    ttACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGt                                                                                 >  1:504464/1‑140 (MQ=255)                                             aTCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAAt                                                                        <  2:909744/140‑1 (MQ=255)                                              tCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATc                                                                       >  2:585356/1‑140 (MQ=255)                                                   tGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGttc                                                                  <  1:494973/140‑1 (MQ=255)                                                   tGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGttc                                                                  <  2:257519/140‑1 (MQ=255)                                                   tGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGttc                                                                  <  2:314896/140‑1 (MQ=255)                                                      tGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGttcttc                                                               >  1:298850/1‑140 (MQ=255)                                                        ttcttcGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCg                                                             >  2:192822/1‑140 (MQ=255)                                                               gTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTAcc                                                      >  2:589653/1‑140 (MQ=255)                                                                tGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCt                                                     >  2:304650/1‑140 (MQ=255)                                                                            tttCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGc                                         >  2:273367/1‑140 (MQ=255)                                                                             ttCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGcc                                        <  1:764927/140‑1 (MQ=255)                                                                                       caGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGa                              >  1:788092/1‑140 (MQ=255)                                                                                                ccTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTaa                     <  1:585356/140‑1 (MQ=255)                                                                                                    cccAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTcc                 >  2:587822/1‑140 (MQ=255)                                                                                                    cccAGGCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTcc                 >  2:203936/1‑140 (MQ=255)                                                                                                      cAGGCCAGATAACCGCTGCTGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTg               <  2:747614/140‑1 (MQ=255)                                                                                                         gCCAGATAACCGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGaa            >  1:436473/1‑140 (MQ=255)                                                                                                                   cGCTGCCGTCAAAGGTCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGAATTCGTCGAtg  >  2:742936/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                             TGCGTACCTCATCCAGTTTGCGCTCTTCCAGCCACTTACGCACAATCGCCTGATGTTCTTCGGTGCATTTGCCGATTTCCTGCATGCAGATCAGACCTTCCCAGGCCAGATAACCGCTGCCGTCAAAGGCCAGTTTGTTCGGTTCGATAACCTCGTTAATAAAATCATCAACGGTTTTATCAATCTGTTCTTCCGATGTACCTTCCGGGAATCGCCATGCCACCGAAAATCCTAATTCCTGGAATTCGTCGATG  >  NC_000913/3101839‑3102092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑